Avenaciolide: a specific inhibitor of glutamate transport in rat liver mitochondria

1970 ◽  
Vol 116 (4) ◽  
pp. 37P-38P ◽  
Author(s):  
J D McGivan ◽  
J B Chappell
Keyword(s):  
Rat Liver ◽  
Specific Inhibitor ◽  
Liver Mitochondria ◽  
Glutamate Transport ◽  
Rat Liver Mitochondria

scholarly journals Quantitative characteristics of glutamate transport in rat liver mitochondria

1973 ◽  
Vol 134 (4) ◽  
pp. 1023-1029 ◽  
Author(s):  
Norah M. Bradford ◽  
J. D. McGivan
Keyword(s):  
Rat Liver ◽  
Transport Process ◽  
Liver Mitochondria ◽  
Glutamate Transport ◽  
Rat Liver Mitochondria ◽  
Bromocresol Purple ◽  
Quantitative Characteristics ◽  
Kinetics Of

1. The kinetics of glutamate transport into mitochondria were determined by using Bromocresol Purple to terminate the transport process. 2. Glutamate transport was found to have a Vmax. of 9.1nmol/min per mg of protein at pH6.9 and 20°C; the Km for glutamate was 4mm. 3. The rate of glutamate deamination in intact mitochondria was tenfold slower than in disrupted mitochondria. 4. These results suggest that glutamate deamination may be controlled by the rate of glutamate transport. Possible consequences of these findings are discussed.

Effects of grisorixin on glutamate transport and oxidation in rat liver mitochondria

Biochimie ◽  
1977 ◽  
Vol 59 (5-6) ◽  
pp. 497-508 ◽  
Author(s):  
Roger Debise ◽  
Yves Briand ◽  
Roger Durand ◽  
Pierre Gachon ◽  
Georges Jeminet
Keyword(s):  
Rat Liver ◽  
Liver Mitochondria ◽  
Glutamate Transport ◽  
Rat Liver Mitochondria

scholarly journals Citreoviridin, a specific inhibitor of the mitochondiral adenosine triphosphatase

1978 ◽  
Vol 170 (3) ◽  
pp. 503-510 ◽  
Author(s):  
P E Linnett ◽  
A D Mitchell ◽  
M D Osselton ◽  
L J Mulheirn ◽  
R B Beechey
Keyword(s):  
Rat Liver ◽  
Adenosine Triphosphatase ◽  
Specific Inhibitor ◽  
Liver Mitochondria ◽  
Mass Action ◽  
Rat Liver Mitochondria ◽  
Mitochondrial Atpase ◽  
Submitochondrial Particles ◽  
Inhibitory Potency ◽  
Dissociation Constant Kd

1. Citreoviridin was a potent inhibitor of the soluble mitochondrial ATPase (adenosine triphosphatase) similar to the closely related aurovertins B and D. 2. Citreoviridin inhibited the following mitochondrial energy-linked reactions also: ADP-stimulated respiration in whole mitochondria from ox heart and rat liver; ATP-driven reduction of NAD+ by succinate; ATP-driven NAD transhydrogenase and ATPase from ox heart submitochondrial particles. 3. The dissociation constant (KD) calculated by a simple law-of-mass-action treatment for the citreoviridin–ATPase complex was 0.5–4.2micron for ox-heart mitochondrial preparations and 0.15micron for rat liver mitochondria. 4. Monoacetylation of citreoviridin decreased its inhibitory potency (KD=2–25micron, ox heart; KD=0.7micron, rat liver). Diacetylation greatly decreased the inhibitory potency (KD=60–215micron, ox heart). 5. Hydrogenation of citreoviridin monoacetate diminished its inhibitory potency considerably. 6. No significant enhancement of fluorescence was observed when citreoviridin interacted with the mitochondrial ATPase.

Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

1971 ◽  
Vol 29 ◽  
pp. 570-571
Author(s):  
E. A. Elfont ◽  
R. B. Tobin ◽  
D. G. Colton ◽  
M. A. Mehlman
Keyword(s):  
Chemical Composition ◽  
Rat Liver ◽  
Future Study ◽  
Mode Of Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Effective Inhibitor ◽  
Biochemical Effects ◽  
Glycerophosphate Dehydrogenase ◽  
Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

Energy and Antioxidant Status of Rat Liver Mitochondria during Hypoxia- Reoxygenation of Different Duration

2016 ◽  
Vol 7 (4) ◽  
pp. 349-361
Author(s):  
Olga A. Gonchar ◽  
Valentina I. Nosar ◽  
Larisa. V. Bratus ◽  
I. N. Tymchenko ◽  
N. N. Steshenko ◽  
...  
Keyword(s):  
Rat Liver ◽  
Antioxidant Status ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Hypoxia Reoxygenation
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