scholarly journals The intermediary role of a steroid 8,14-dien-3β-ol system in ergosterol biosynthesis

1969 ◽  
Vol 115 (2) ◽  
pp. 135-137 ◽  
Author(s):  
M Akhtar ◽  
W. A. Brooks ◽  
I. A. Watkinson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ergosterol Biosynthesis ◽  
Methyl Group ◽  
Diene System ◽  
Intermediary Role

1. A mechanism for the removal of the 14α-methyl group in ergosterol biosynthesis that involves the intermediacy of an 8,14-diene system is outlined. 2. In accordance with the requirements of this scheme, it is shown that 5α-ergosta-8,14-dien-3β-ol is converted into ergosterol by Saccharomyces cerevisiae.

scholarly journals Eukaryotic Wobble Uridine Modifications Promote a Functionally Redundant Decoding System

2008 ◽  
Vol 28 (10) ◽  
pp. 3301-3312 ◽  
Author(s):  
Marcus J. O. Johansson ◽  
Anders Esberg ◽  
Bo Huang ◽  
Glenn R. Björk ◽  
Anders S. Byström
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Side Chain ◽  
Side Chains ◽  
Methyl Group ◽  
Wobble Position ◽  
Naturally Occurring

ABSTRACT The translational decoding properties of tRNAs are modulated by naturally occurring modifications of their nucleosides. Uridines located at the wobble position (nucleoside 34 [U34]) in eukaryotic cytoplasmic tRNAs often harbor a 5-methoxycarbonylmethyl (mcm5) or a 5-carbamoylmethyl (ncm5) side chain and sometimes an additional 2-thio (s2) or 2′-O-methyl group. Although a variety of models explaining the role of these modifications have been put forth, their in vivo functions have not been defined. In this study, we utilized recently characterized modification-deficient Saccharomyces cerevisiae cells to test the wobble rules in vivo. We show that mcm5 and ncm5 side chains promote decoding of G-ending codons and that concurrent mcm5 and s2 groups improve reading of both A- and G-ending codons. Moreover, the observation that the mcm5U34- and some ncm5U34-containing tRNAs efficiently read G-ending codons challenges the notion that eukaryotes do not use U-G wobbling.

Nuclear Demethylation and C-24 Alkylation During Ergosterol Biosynthesis in Saccharomyces cerevisiae

1975 ◽  
Vol 53 (8) ◽  
pp. 881-889 ◽  
Author(s):  
M. Fryberg ◽  
L. Avruch ◽  
A. C. Oehlschlager ◽  
A. M. Unrau
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ergosterol Biosynthesis ◽  
Aerobic Growth ◽  
Equal Importance ◽  
Major Route ◽  
Commercial Yeast

The role of 4,4-dimethylzymosterol (3), 4,4-dimethylfecosterol (4) and 31-norlanosterol (5) in the biosynthesis of ergosterol in Saccharomyces cerevisiae has been investigated. The synthesis of 4 and 5 coupled with the availability of 3 facilitated a search for these sterols in commercial yeast sterol concentrates, fresh laboratory grown yeast and fresh brewery grown yeast. Sterol 4 was not detected in any of these mixtures whereas 5 was found in the first and last and 3 was present in all three sources investigated. Investigation of incorporation of [2-3H]lanosterol into 3, 4 and 5 revealed significant incorporation into 3 but neither 4 nor 5. This observation suggests the principle pathway for ergosterol biosynthesis initially involved 1 → 3 → 7.Incubation of a mixture of [2,4-3H]zymosterol and [26,27-14C]lanosterol with S. cerevisiae revealed that during the initial phases of aerobic growth the major route from 7 to ergosterol involves zymosterol (11) but as 11 accumulates 4α-methyl-24-methylenezymosterol (8) assumes equal importance.

Protective role of l ‐threonine against cadmium toxicity in Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Linru Huang ◽  
Zhijia Fang ◽  
Jian Gao ◽  
Jingwen Wang ◽  
Yongbin Li ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cadmium Toxicity ◽  
Protective Role

scholarly journals Suppressor Analysis of the Saccharomyces cerevisiae Gene REC104 Reveals a Genetic Interaction With REC102

Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1261-1272 ◽  
Author(s):  
Laura Salem ◽  
Natalie Walter ◽  
Robert Malone
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Meiotic Recombination ◽  
Null Allele ◽  
Genetic Interaction ◽  
In Vitro Mutagenesis ◽  
Conditional Mutations ◽  
Suppressor Analysis

Abstract REC104 is a gene required for the initiation of meiotic recombination in Saccharomyces cerevisiae. To better understand the role of REC104 in meiosis, we used an in vitro mutagenesis technique to create a set of temperature-conditional mutations in REC104 and used one ts allele (rec104-8) in a screen for highcopy suppressors. An increased dosage of the early exchange gene REC102 was found to suppress the conditional recombinational reduction in rec104-8 as well as in several other conditional rec104 alleles. However, no suppression was observed for a null allele of REC104, indicating that the suppression by REC102 is not “bypass” suppression. Overexpression of the early meiotic genes REC114, RAD50, HOP1, and RED1 fails to suppress any of the rec104 conditional alleles, indicating that the suppression might be specific to REC102.

Research on the influence of relation embeddedness on innovation performance of manufacturing supply chain alliances using expert fuzzy rule –intermediary role of shared mental model

2021 ◽  
pp. 1-8
Author(s):  
Guo Lifang ◽  
Cui Yuwen ◽  
Wu Yamin ◽  
Ma Jiaqi
Keyword(s):  
Supply Chain ◽  
Mental Model ◽  
Fuzzy Rule ◽  
Innovation Performance ◽  
Market Demand ◽  
Shared Mental Model ◽  
Rule Based System ◽  
Manufacturing Supply Chain ◽  
Intermediary Role

The innovation and development of manufacturing supply chain alliance is an important way for enterprises to meet the increasing market demand and maintain the competitive advantage. From the perspective of embeddedness, the research model of relation embeddedness on innovation performance of manufacturing supply chain was constructed based on AMOS. Shared mental model was selected as intermediary variable to study the influence of relation embeddedness, shared mental model and innovation performance of manufacturing supply chain alliances. Expert fuzzy rule based system is utilized for measuring the performance of manufacturing supply chain alliances. The conclusion shows that relation embeddedness is significantly positive shared mental model and innovation performance. Shared mental model is positively affects alliance innovation performance and plays a part of intermediary role between relational embedding and alliance innovation performance. Practice implicates that enhance the level of relation embeddedness can promote the formation of shared mental model and improve the innovation performance of manufacturing supply chain alliance.

Role of Oxidative Phosphorylation in Histatin 5-Induced Cell Death in Saccharomyces cerevisiae

2004 ◽  
Vol 26 (23) ◽  
pp. 1781-1785 ◽  
Author(s):  
Kris De Smet ◽  
Rieka Reekmans ◽  
Roland Contreras
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Death ◽  
Oxidative Phosphorylation ◽  
Histatin 5
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