scholarly journals Collagen synthesis and carbohydrate metabolism of rachitic bone

1968 ◽  
Vol 109 (1) ◽  
pp. 101-106 ◽  
Author(s):  
C. R. Paterson ◽  
P. Fourman
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Collagen Synthesis ◽  
Direct Action ◽  
Bone Collagen ◽  
Salt Solutions ◽  
Normal Bone ◽  
Deficiency Rickets

1. This paper reports studies on the metabolism of bone from normal chicks and from chicks with vitamin D-deficiency rickets. Both in vitro and in vivo there was an increased incorporation of [14C]proline into collagen hydroxyproline by rachitic bone. The proportion of the collagen that was soluble in cold salt solutions was greater with the rachitic bone. These results show that in rickets there is an increased synthesis of bone collagen, but they do not provide any evidence of a defect in the maturation of collagen. 2. Rachitic bone incubated aerobically in vitro consumed more glucose and released more lactate than normal bone. Bone from rachitic chicks treated with vitamin D 48hr. previously had rates of glycolysis that were nearly normal. Though we were unable to show any direct action of vitamin D in vitro, we consider that vitamin D probably has a direct action on bone, possibly related to matrix biosynthesis.

scholarly journals A15 VITAMIN D DEFICIENCY PROMOTES INTESTINAL AUTOPHAGY DYSFUNCTION VIA EPIGENETIC REGULATION INVOLVING MIR142-3P IN VITRO AND IN VIVO

2018 ◽  
Vol 1 (suppl_2) ◽  
pp. 23-24
Author(s):  
D M Bronte-Tinkew ◽  
F Dang ◽  
A Hsieh ◽  
L H McGillis ◽  
I Verapalan ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Epigenetic Regulation

Diet-induced vitamin D deficiency reduces skeletal muscle mitochondrial respiration

2021 ◽  
Vol 249 (2) ◽  
pp. 113-124
Author(s):  
Stephen P Ashcroft ◽  
Gareth Fletcher ◽  
Ashleigh M Philp ◽  
Carl Jenkinson ◽  
Shatarupa Das ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Protein Content ◽  
Vitamin D Deficiency ◽  
Mitochondrial Function ◽  
Mitochondrial Respiration ◽  
Citrate Synthase ◽  
Current Evidence

Vitamin D deficiency is associated with symptoms of skeletal muscle myopathy including muscle weakness and fatigue. Recently, vitamin D-related metabolites have been linked to the maintenance of mitochondrial function within skeletal muscle. However, current evidence is limited to in vitro models and the effects of diet-induced vitamin D deficiency upon skeletal muscle mitochondrial function in vivo have received little attention. In order to examine the role of vitamin D in the maintenance of mitochondrial function in vivo, we utilised an established model of diet-induced vitamin D deficiency in C57BL/6J mice. Mice were either fed a control diet (2200 IU/kg i.e. vitamin D replete) or a vitamin D-deplete (0 IU/kg) diet for periods of 1, 2 and 3 months. Gastrocnemius muscle mitochondrial function and ADP sensitivity were assessed via high-resolution respirometry and mitochondrial protein content via immunoblotting. As a result of 3 months of diet-induced vitamin D deficiency, respiration supported via complex I + II (CI + IIP) and the electron transport chain (ETC) were 35 and 37% lower when compared to vitamin D-replete mice (P < 0.05). Despite functional alterations, citrate synthase activity, AMPK phosphorylation, mitofilin, OPA1 and ETC subunit protein content remained unchanged in response to dietary intervention (P > 0.05). In conclusion, we report that 3 months of diet-induced vitamin D deficiency reduced skeletal muscle mitochondrial respiration in C57BL/6J mice. Our data, when combined with previous in vitro observations, suggest that vitamin D-mediated regulation of mitochondrial function may underlie the exacerbated muscle fatigue and performance deficits observed during vitamin D deficiency.

Role of Vitamin D in Autism Spectrum Disorder

2020 ◽  
Vol 25 (41) ◽  
pp. 4357-4367 ◽  
Author(s):  
Loai Alzghoul
Keyword(s):  
Autism Spectrum Disorder ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Autism Spectrum ◽  
Vitamin D Supplementation ◽  
Spectrum Disorder ◽  
Vitamin D Levels

: Autism spectrum disorder (ASD) is a pervasive developmental disorder with heterogeneous etiology. Vitamin D can function as a fat-soluble vitamin as well as a hormone, and can exert its effect through both genomic and non-genomic mechanisms. In the last decades, several studies have examined the relationship between vitamin D levels and ASD. These studies demonstrated that low vitamin D status in early development has been hypothesized as an environmental risk factor for ASD. Both in vivo and in vitro studies have demonstrated that vitamin D deficiency in early life can alter brain development, dysregulates neurotransmitter balance in the brain, decreases body and brain antioxidant ability, and alters the immune system in ways that resemble pathological features commonly seen in ASD. In this review, we focused on the association between vitamin D and ASD. In addition, the above-mentioned mechanisms of action that link vitamin D deficiency with ASD were also discussed. Finally, clinical trials of vitamin D supplementation treatment of ASD have also been discussed.

The influence of lipid droplet size on the oral bioavailability of vitamin D2encapsulated in emulsions: an in vitro and in vivo study

2017 ◽  
Vol 8 (2) ◽  
pp. 767-777 ◽  
Author(s):  
L. Salvia-Trujillo ◽  
B. Fumiaki ◽  
Y. Park ◽  
D. J. McClements
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Health Effects ◽  
Droplet Size ◽  
Oral Bioavailability ◽  
Functional Foods ◽  
Adverse Health Effects ◽  
Lipid Droplet Size

Vitamin D deficiency is prevalent in some populations leading to adverse health effects, and therefore there is a need to supplement functional foods and beverages with this important micronutrient.

scholarly journals Vitamin D Regulation of OX40 Ligand in Immune Responses to Aspergillus fumigatus

2013 ◽  
Vol 81 (5) ◽  
pp. 1510-1519 ◽  
Author(s):  
Nikki Lynn Hue Nguyen ◽  
Kong Chen ◽  
Jeremy Mcaleer ◽  
Jay K. Kolls
Keyword(s):  
Vitamin D ◽  
Aspergillus Fumigatus ◽  
Vitamin D Deficiency ◽  
Costimulatory Molecule ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Content Type ◽  
Ox40 Ligand ◽  
Th2 Responses

ABSTRACTOX40 ligand (OX40L) is a costimulatory molecule involved in Th2 allergic responses. It has been shown that vitamin D deficiency is associated with increased OX40L expression in peripheral CD11c+cells and controls Th2 responses toAspergillus fumigatusin vitroin cystic fibrosis (CF) patients with allergic bronchopulmonary aspergillosis (ABPA). To investigate if vitamin D deficiency regulated OX40L and Th2 responsesin vivo, we examined the effect of nutritional vitamin D deficiency on costimulatory molecules in CD11c+cells andA. fumigatus-induced Th2 responses. Vitamin D-deficient mice showed increased expression of OX40L on lung CD11c+cells, and OX40L was critical for enhanced Th2 responses toA. fumigatusin vivo. Inin vitroassays, vitamin D treatment led to vitamin D receptor (VDR) binding in the promoter region of OX40L and significantly decreased the promoter activity of the OX40L promoter. In addition, vitamin D altered NF-κB p50 binding in the OX40L promoter that may be responsible for repression of OX40L expression. These data show that vitamin D can act directly on OX40L, which impacts Th2 responses and supports the therapeutic use of vitamin D in diseases regulated by OX40L.

scholarly journals Diet-Induced Vitamin D Deficiency Results in Reduced Skeletal Muscle Mitochondrial Respiration in C57BL/6J Mice

2020 ◽  
Author(s):  
Stephen P. Ashcroft ◽  
Gareth Fletcher ◽  
Ashleigh M. Philp ◽  
Philip J. Atherton ◽  
Andrew Philp
Keyword(s):  
Skeletal Muscle ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Mitochondrial Function ◽  
Dietary Intervention ◽  
Mitochondrial Protein ◽  
Current Evidence ◽  
Performance Deficits

AbstractVitamin D deficiency is known to be associated with symptoms of skeletal muscle myopathy including muscle weakness and fatigue. Recently, vitamin D related metabolites have been linked to the maintenance of mitochondrial function within skeletal muscle. However, current evidence is limited to in vitro models and the effects of diet-induced vitamin D deficiency upon skeletal muscle mitochondrial function in vivo have received little attention. In order to examine the role of vitamin D in the maintenance of mitochondrial function in vivo, we utilised an established model of diet-induced vitamin D deficiency in C57BL/6J mice. Mice were fed either a control (2,200 IU/kg) or a vitamin D deplete (0 IU/kg) diet for periods of 1-, 2- and 3-months. Skeletal muscle mitochondrial function and ADP sensitivity were assessed via high-resolution respirometry and mitochondrial protein content via immunoblotting. As a result of 3-month of diet-induced vitamin D deficiency, respiration supported via CI+IIP and ETC were 35% and 37% lower when compared to vitamin D replete mice (P < 0.05). Despite functional alterations, the protein expression of electron transfer chain subunits remained unchanged in response to dietary intervention (P > 0.05). In conclusion, we report that 3-months of diet-induced vitamin D deficiency reduced skeletal muscle mitochondrial function in C57BL/6J mice. Our data, when combined with previous in vitro observations, suggests that vitamin D mediated regulation of mitochondrial function may underlie the exacerbated muscle fatigue and performance deficits observed during vitamin D deficiency.

Biological and biomedical applications of collagenous biomaterials

1990 ◽  
Vol 48 (3) ◽  
pp. 856-857
Author(s):  
Yasushi P. Kato ◽  
Michael G. Dunn ◽  
Frederick H. Silver ◽  
Arthur J. Wasserman
Keyword(s):  
Biomedical Applications ◽  
Soft Tissues ◽  
Collagen Fibers ◽  
Bone Collagen ◽  
Cover Slip ◽  
Fibroblast Migration ◽  
Cellular Expression ◽  
Defect Repair

Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support fibroblast growth in vitro, and second as a demineralized bone/collagen sponge for radial bone defect repair in vivo.For the in vitro study, collagen fibers were prepared as described previously. Primary rat tendon fibroblasts (1° RTF) were isolated and cultured for 5 days on 1 X 15 mm sterile cover slips. Six to seven collagen fibers, were glued parallel to each other onto a circular cover slip (D=18mm) and the 1 X 15mm cover slip populated with 1° RTF was placed at the center perpendicular to the collagen fibers. Fibroblast migration from the 1 x 15mm cover slip onto and along the collagen fibers was measured daily using a phase contrast microscope (Olympus CK-2) with a calibrated eyepiece. Migratory rates for fibroblasts were determined from 36 fibers over 4 days.

Akalzämische Vitamin-D-Analoga zeigen antifibrotische Effekte in vitro in hepatischen Sternzellen und in vivo im CCl4-Mausmodell

2019 ◽  
Author(s):  
FP Reiter ◽  
L Ye ◽  
F Bösch ◽  
R Wimmer ◽  
R Artmann ◽  
...  
Keyword(s):  
Vitamin D
Sign in / Sign up

Export Citation Format

Share Document