scholarly journals A method for the quantitative determination of N-terminal amino acids based on radioisotope dilution

1968 ◽  
Vol 107 (5) ◽  
pp. 728-729 ◽  
Author(s):  
G. L. Callewaert ◽  
C. A. Vernon
Keyword(s):  
Amino Acids ◽  
Quantitative Determination ◽  
Terminal Amino

scholarly journals Quantitative determination of N-terminal amino acids of peptides and proteins with cobalt(III) chelates

1976 ◽  
Vol 153 (1) ◽  
pp. 137-138 ◽  
Author(s):  
K W Bentley
Keyword(s):  
Amino Acids ◽  
Quantitative Determination ◽  
Peptide Bond ◽  
Small Peptides ◽  
Acidic Hydrolysis ◽  
Terminal Amino ◽  
A Chain ◽  
Glucagon And Insulin

Quantitative N-terminal peptide-bond hydrolysis with the cis-beta-hydroxyaquo(triethylenetetramine) cobal (III) ion, i.e. β-[Co(trien)(OH)(OH2)]2+, is reported. The method has been demonstrated with 20 small peptides, a hexapeptide, bradykinin, insulin A chain (oxidized), glucagon and insulin. The procedure involves no acidic hydrolysis step and thus no destruction of labile amino acids.

scholarly journals The Determination of N-terminal Amino Acids during the Conversion of Fibrinogen to Fibrin.

1957 ◽  
Vol 11 ◽  
pp. 194-195 ◽  
Author(s):  
Birger Blombäck ◽  
Ikuo Yamashina ◽  
S. Ross ◽  
J. Schliack ◽  
L. Reio
Keyword(s):  
Amino Acids ◽  
Terminal Amino

scholarly journals Determination of Caffeine and Taurine Contents in Energy Drinks by HPLC-UV

2016 ◽  
Vol 9 ◽  
pp. 66-73 ◽  
Author(s):  
Krishna Prasad Rai ◽  
Hasta Bahadur Rai ◽  
Santosh Dahal ◽  
Saroj Chaudhary ◽  
Suraj Shrestha
Keyword(s):  
Amino Acids ◽  
Pilot Study ◽  
Quantitative Determination ◽  
Physiological Responses ◽  
Alcoholic Beverage ◽  
Energy Drinks ◽  
Energy Drink ◽  
Young Generation ◽  
Caffeine Content

Energy drinks are non-alcoholic beverage intended to enhance the psycho-physiological responses in human, which is especially popular among young generation in Nepal. It is normally high caffeinated drink added with other ingredients such as carbohydrates, amino acids, B-group of vitamins etc. In this study, 10 brands of energy drink available in Nepalese markets were taken then analyzed for quantitative determination of Caffeine and Taurine by HPLC-UV method. From the result obtained, pH and TSS values of energy drinks were found in the range of 2.96-3.81 and 6.64-18.21 respectively. Likewise, the Caffeine and Taurine content in same samples were found in the range of not detected (ND) to 35.78 mg/100 ml and ND to 387.5 mg/100 ml respectively. Only the 6 samples out of 10 were confi rmed caffeine content as per claimed in label, while only 3 samples were confi rmed for Taurine content as per label claimed. Based on this pilot study, the majority of samples did not meet the label claims in term of Caffeine and Taurine, which apparently indicated the misbranding of such drinks. Since, there is no any regulation for such energy drinks in Nepal, it seems to be a great challenge for regulation of their safety and misbranding.

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