scholarly journals Mass-spectrometric evidence for methyl and ethyl esters of long-chain fatty acids in ox pancreas

1968 ◽  
Vol 107 (2) ◽  
pp. 318-319 ◽  
Author(s):  
J Skorepa ◽  
P Hrabák ◽  
P Mares ◽  
A Linnarson
Keyword(s):  
Fatty Acids ◽  
Mass Spectrometric ◽  
Ethyl Esters ◽  
Long Chain Fatty Acids ◽  
Mass Spectrometric Evidence ◽  
Long Chain ◽  
Chain Fatty Acids

HPLC with spectrophotometric or mass spectrometric detection for quantifying very-long chain fatty acids in human plasma and its association with cardiac risk factors

2021 ◽  
pp. 000456322110071
Author(s):  
Rojeet Shrestha ◽  
Zhen Chen ◽  
Zijun Gao ◽  
Yifan Chen ◽  
Emiko Okada ◽  
...  
Keyword(s):  
Risk Factors ◽  
Fatty Acids ◽  
Human Plasma ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Selected Reaction Monitoring ◽  
Elevated Concentration ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

Background We developed and compared two liquid chromatography methods, one with UV/Visible spectrophotometric detection (HPLC) and the other with mass spectrometric detection (LC-MS), for quantifying very-long chain fatty acids (VLCFA) in human plasma. Association of VLCFA with various cardiovascular risk factors were evaluated. Method Fasting blood samples were collected from 541 human volunteers (242 men and 299 women; mean age ±SD, 58.9 ± 12.4 years), including 429 and 112 individuals with and without hypertriglyceridemia, respectively. Esterified VLCFA were saponified and derivatized with 2-nitrophenylhydrazine. Separation of VLCFA species was achieved with C4 Mightysil column (HPLC) and Ascentis Express Phenyl-Hexyl column (LC-MS) followed by spectrophotometric and selected-reaction monitoring mode of mass spectrometric detection, respectively. Results The HPLC assay of VLCFA was precise with intra-assay imprecision of 2.5% to 6.9% and inter-assay imprecision of 3.2% to 9.5%. Moreover, there was an excellent correlation (r > 0.96) between HPLC and LC-MS methods. The 95 percentile reference intervals (RI; upper limit) of VLCFA were determined to be 41.3 µmol/L in healthy volunteers. Plasma VLCFA were significantly correlated with triglycerides (Spearman’s ρ = 0.306, P <  0.001) and total cholesterol (Spearman’s ρ = 0.251, P <  0.001). All species of VLCFA were significantly elevated in hypertriglyceridaemic individuals compared with control. Conclusion We established LC-based assays of VLCFA with either spectrophotometry or mass spectrometry as a detection system. Hypertriglyceridaemia is significantly associated with elevated concentration of each species of VLCFA.

scholarly journals Characterization of enzymes involved in formation of ethyl esters of long-chain fatty acids in humans

2001 ◽  
Vol 42 (7) ◽  
pp. 1025-1032 ◽  
Author(s):  
Margareta A. Diczfalusy ◽  
Ingemar Björkhem ◽  
Curt Einarsson ◽  
Carl-Gustaf Hillebrant ◽  
Stefan E.H. Alexson
Keyword(s):  
Fatty Acids ◽  
Ethyl Esters ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids
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