scholarly journals Post-mortem glycolysis in ox skeletal muscle. Effect of temperature on the concentrations of glycolytic intermediates and cofactors

1967 ◽  
Vol 105 (1) ◽  
pp. 127-136 ◽  
Author(s):  
R. P. Newbold ◽  
R. K. Scopes
Keyword(s):  
Skeletal Muscle ◽  
Creatine Phosphate ◽  
Effect Of Temperature ◽  
Soluble Phosphate ◽  
Hexose Monophosphate ◽  
Post Mortem ◽  
Total Acid ◽  
Atp Turnover ◽  
Glycolytic Intermediates ◽  
Phosphofructokinase Activity

1. Post-mortem changes in the concentrations of the following compounds in ox sternomandibularis muscles stored in nitrogen at 1°, 5° and 15° are reported: Pi creatine phosphate, hexose monophosphates, fructose diphosphate, triose phosphates, α-glycerophosphate, phosphoglycerates, lactate, ATP, ADP, AMP, NAD+ and total nucleotides. Some results obtained with muscles stored at 37° are included. 2. At the time the muscles were placed at controlled temperatures (about 1·5hr. post mortem) the phosphorus in the compounds measured accounted for 91±6% (s.d.) of the total acid-soluble phosphate. 3. The results indicated that at all temperatures the activities of the phosphorylase and phosphofructokinase steps limited the rate and the extent of post-mortem glycolysis. 4. The large variations in hexose monophosphate concentrations during storage indicated that the ratio of phosphorylase to phosphofructokinase activity varied considerably with time and temperature. 5. Between 3·5 and 7hr. post mortem the rates of glycolysis and of ATP turnover were not slower at 5° that at 15°, and were probably faster at 1°. The significance of this finding is discussed.

Comparison in control and severely diabetic, alloxanized rats of phosphate fractions, glycogen and fat

1959 ◽  
Vol 196 (6) ◽  
pp. 1246-1249 ◽  
Author(s):  
Clarissa H. Beatty ◽  
Ruth D. Peterson ◽  
Rose Mary Bocek ◽  
Edward S. West
Keyword(s):  
Body Weight ◽  
Inorganic Phosphate ◽  
Trichloroacetic Acid ◽  
Adenosine Diphosphate ◽  
Creatine Phosphate ◽  
Soluble Phosphate ◽  
Total Acid ◽  
Control Series ◽  
Noncollagenous Protein ◽  
Total Muscle

Homogeneous aliquots of powdered muscle were analyzed and levels of constituents calculated on a fat-free basis. When compared to muscle from control rats, muscle from severely diabetic, alloxanized rats showed a decrease in creatine phosphate, total acid soluble phosphate and total phosphate, with no change in inorganic phosphate, adenosine diphosphate plus adenosine triphosphate, or total acid soluble phosphate minus 7-minute hydrolyzable phosphate, creatine, noncollagenous protein or total nitrogen. There was an increase in the trichloroacetic acid extractable glycogen value in the muscle of the alloxan diabetic as compared to the control series. The concentration of fat in the muscle itself was higher in the diabetic than in the control series, although the total muscle fat had decreased in the diabetic series due to the decrease in percentage of body weight represented by muscle.

scholarly journals Post-mortem glycolysis in ox skeletal muscle. Effect of pre-rigor freezing and thawing on the intermediary metabolism

1968 ◽  
Vol 109 (2) ◽  
pp. 197-202 ◽  
Author(s):  
R. K. Scopes ◽  
R. P. Newbold
Keyword(s):  
Carbon Dioxide ◽  
Creatine Phosphate ◽  
Slow Freezing ◽  
Hexose Monophosphate ◽  
Post Mortem ◽  
Solid Carbon ◽  
Freezing And Thawing ◽  
Solid Carbon Dioxide ◽  
Triose Phosphate ◽  
Fast Freezing

1. Ox sternomandibularis muscle was ‘slow-frozen’ by placing it in air at −22° or ‘fast-frozen’ by immersion in liquid air or acetone–solid carbon dioxide. In all cases muscles were frozen pre-rigor. Changes in length, pH and the concentrations of Pi, creatine phosphate, hexose monophosphate (glucose 1-phosphate+glucose 6-phosphate+fructose 6-phosphate), fructose diphosphate (fructose 1,6-diphosphate+½ triose phosphate), lactate, ATP, ADP, AMP and NAD+ during freezing and during subsequent thawing were determined. In addition some measurements were made of the changes in α-glycerophosphate, 3-phosphoglycerate, 2-phosphoglycerate, phosphoenolpyruvate and pyruvate concentrations during slow freezing. 2. Appreciable shortening and marked changes in chemical composition took place during slow freezing but not during fast freezing. 3. During slow freezing the hexose monophosphate concentration fell and fructose 1,6-diphosphate and triose phosphate increased substantially. Increases also took place in 3-phosphoglycerate, 2-phosphoglycerate and phosphoenolpyruvate, but not in pyruvate. 4. On thawing, most of the chemical changes were similar to those in unfrozen muscle post mortem, but took place much more rapidly; loss of NAD+ was particularly rapid. Fast-frozen muscle metabolized at a faster rate on thawing than did slow-frozen muscle. 5. The overall changes in length during freezing and thawing were about the same in slow-frozen as in fast-frozen muscle.

Differences of post-mortem ATP turnover in skeletal muscle of normal and heterozygote malignant-hyperthermia pigs: Comparison of 31P-NMR and analytical biochemical measurements

Meat Science ◽  
1995 ◽  
Vol 39 (1) ◽  
pp. 43-57 ◽  
Author(s):  
Birthe Moesgaard ◽  
Bjørn Quistorff ◽  
Vibeke Grøsfjeld Christensen ◽  
Ib Therkelsen ◽  
Preben Fogd Jørgensen
Keyword(s):  
Skeletal Muscle ◽  
Malignant Hyperthermia ◽  
31P Nmr ◽  
Post Mortem ◽  
Atp Turnover ◽  
Biochemical Measurements

scholarly journals Skeletal muscle ATP turnover and muscle fiber conduction velocity are elevated at higher muscle temperatures during maximal power output development in humans

2006 ◽  
Vol 290 (2) ◽  
pp. R376-R382 ◽  
Author(s):  
Stuart R. Gray ◽  
Giuseppe De Vito ◽  
Myra A. Nimmo ◽  
Dario Farina ◽  
Richard A. Ferguson
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Power Output ◽  
Effect Of Temperature ◽  
Muscle Temperature ◽  
Maximal Power ◽  
Maximal Power Output ◽  
Muscle Fiber Conduction Velocity ◽  
Atp Turnover

The effect of temperature on skeletal muscle ATP turnover and muscle fiber conduction velocity (MFCV) was studied during maximal power output development in humans. Eight male subjects performed a 6-s maximal sprint on a mechanically braked cycle ergometer under conditions of normal (N) and elevated muscle temperature (ET). Muscle temperature was passively elevated through the combination of hot water immersion and electric blankets. Anaerobic ATP turnover was calculated from analysis of muscle biopsies obtained before and immediately after exercise. MFCV was measured during exercise using surface electromyography. Preexercise muscle temperature was 34.2°C (SD 0.6) in N and 37.5°C (SD 0.6) in ET. During ET, the rate of ATP turnover for phosphocreatine utilization [temperature coefficient (Q10) = 3.8], glycolysis (Q10 = 1.7), and total anaerobic ATP turnover [Q10 = 2.7; 10.8 (SD 1.9) vs. 14.6 mmol·kg−1 (dry mass)·s−1 (SD 2.3)] were greater than during N ( P < 0.05). MFCV was also greater in ET than in N [3.79 (SD 0.47) to 5.55 m/s (SD 0.72)]. Maximal power output (Q10 = 2.2) and pedal rate (Q10 = 1.6) were greater in ET compared with N ( P < 0.05). The Q10 of maximal and mean power were correlated ( P < 0.05; R = 0.82 and 0.85, respectively) with the percentage of myosin heavy chain type IIA. The greater power output obtained with passive heating was achieved through an elevated rate of anaerobic ATP turnover and MFCV, possibly due to a greater effect of temperature on power production of fibers, with a predominance of myosin heavy chain IIA at the contraction frequencies reached.

Regulation of anaerobic ATP-generating pathways in trout fast-twitch skeletal muscle

1987 ◽  
Vol 253 (1) ◽  
pp. R186-R194 ◽  
Author(s):  
G. P. Dobson ◽  
W. S. Parkhouse ◽  
P. W. Hochachka
Keyword(s):  
Skeletal Muscle ◽  
Phosphoglycerate Kinase ◽  
Fold Increase ◽  
Glycolytic Pathway ◽  
Swimming Velocity ◽  
Glycogen Depletion ◽  
Atp Turnover ◽  
Relative Contribution ◽  
Glycolytic Intermediates ◽  
Fast Twitch

In the process of defining the recruitment of fuel and pathway selection in rainbow trout fast-twitch white skeletal muscle, it was clear that the near-maximal myosin adenosinetriphosphatase activity during a 10-s sprint was supported solely by phosphocreatine hydrolysis. A conservative estimate of the ATP turnover was 188 mumol X g wet wt-1 X min-1. It was not until the rate and force of contraction decreased that the relative contribution of anaerobic glycogenolysis became increasingly important. Over a 10-min period of burst swimming at approximately 120% of maximum aerobic steady-state swimming velocity of trout determined in a Brett-type swim tunnel, fatigue was associated with the near-depletion of glycogen in white muscle. The ATP turnover supported by anaerobic glycogenolysis was 78 mumol X g wet wt-1 X min-1. The glycolytic pathway appeared functional at this time with control sites being identified at hexokinase and phosphofructokinase (PFK-1). PFK-1 did not appear to be inhibited by low muscle pH (pH 6.66). In another exercise protocol lasting 30 min, complete exhaustion was related to glycogen depletion. The sum of all glycolytic intermediates from glucose 6-phosphate to pyruvate at exhaustion decreased by a dramatic 80% compared with the 25% decrease for the 10-min fatigue swimming protocol. This large depletion of glycolytic intermediates was accompanied by an 80% fall in ATP, a 70-80% reduction in the ATP/ADP and phosphorylation potential, and a 2.5-fold increase in the NAD/NADH. Associated with these changes was a marked displacement of the phosphoglycerate kinase (PGK), and the combined glyceraldehyde-3-phosphate dehydrogenase-PGK reactions from thermodynamic equilibrium. As a general conclusion, fatigue and exhaustion should be viewed as a multicomponent biochemical process in response to low glycogen and not leveled at one particular step of the glycolytic pathway.

THE EFFECT OF ETHER, PENTOBARBITAL, AND DECAPITATION ON VARIOUS METABOLITES OF RAT SKELETAL MUSCLE

1967 ◽  
Vol 45 (9) ◽  
pp. 1323-1327 ◽  
Author(s):  
Ana Marquez-Julio ◽  
I. W. French
Keyword(s):  
Skeletal Muscle ◽  
Creatine Phosphate ◽  
Hexose Monophosphate ◽  
Rat Skeletal Muscle ◽  
Pentobarbital Anesthesia ◽  
Anesthetized Rats ◽  
Lactate And Pyruvate

The concentrations of creatine phosphate, ATP, ADP, AMP, lactate, pyruvate, hexose monophosphate, and glycogen were examined in skeletal muscle obtained from decapitated rats, or animals anesthetized with ether or pentobarbital.The concentrations of ATP, creatine phosphate, and glycogen were highest in muscles from pentobarbital-treated rats, whereas those of AMP, ADP, lactate, and pyruvate were the lowest in these animals. The data suggest that changes in the concentration of metabolites in skeletal muscle were less when the tissue was isolated under pentobarbital anesthesia than when it was isolated from decapitated or ether-anesthetized rats.

scholarly journals Effect of Dietary Magnesium on Post Mortem Phosphocreatine Utilization in Skeletal Muscle of Swine: A Non-Invasive Study Using 31P-NMR Spectroscopy

1993 ◽  
Vol 34 (4) ◽  
pp. 397-404
Author(s):  
B. Moesgaard ◽  
I. Errebo Larsen ◽  
B. Quistorff ◽  
I. Therkelsen ◽  
V. Grøsfjeld Christensen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Nmr Spectroscopy ◽  
31P Nmr ◽  
31P Nmr Spectroscopy ◽  
Post Mortem ◽  
Non Invasive ◽  
Dietary Magnesium

Activities of selected antioxidative, hydrolytic and hexose monophosphate shunt enzymes of skeletal muscle in neuromuscular diseases

2009 ◽  
Vol 69 (S98) ◽  
pp. 176-177
Author(s):  
V. V. Myllylä ◽  
T. E. S. Takala ◽  
U. Tolonen ◽  
A. Salminen ◽  
M. Kihlström ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Neuromuscular Diseases ◽  
Hexose Monophosphate ◽  
Hexose Monophosphate Shunt
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