scholarly journals Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria

1967 ◽  
Vol 104 (1) ◽  
pp. 258-262 ◽  
Author(s):  
KS Kirby ◽  
E Fox-Carter ◽  
M Guest
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Ribosomal Ribonucleic Acid

Ribosomal ribonucleic acid cistron homologies among Hyphomicrobium and various other bacteria

1977 ◽  
Vol 23 (4) ◽  
pp. 478-481 ◽  
Author(s):  
Richard L. Moore
Keyword(s):  
Cell Wall ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Gram Positive ◽  
Gram Negative ◽  
Group Iii ◽  
Hybrid Formation ◽  
Group I ◽  
Ribosomal Ribonucleic Acid ◽  
Taxonomic Implications

The extent of hybrid formation between the ribosomal ribonucleic acid (r-RNA) of Hyphomicrobium strain B-522 and deoxyribonucleic acid (DNA) from bacteria of 21 different genera was examined. Three generalized groupings were formed. Group I (72–100%) consisted entirely of other strains of Hyphomicrobium. Representatives of the genera Rhodopseudomonas, Chromatium, Caulobacter, Prosthecomicrobium, Rhodomicrobium, Hyphomonas, and Hyphomicrobium made up group II (49–69%). The remaining Gram-negative, Gram-positive, and cell wall – less bacteria fell into group III (12–40%). The taxonomic implications of these results are discussed.

scholarly journals Deoxyribonucleic acid–ribonucleic acid hybridization. Annealing and quantitative recovery of intact ribosomal ribonucleic acid molecules from hybrids

1969 ◽  
Vol 115 (2) ◽  
pp. 287-294 ◽  
Author(s):  
Michael Fry ◽  
Michael Artman
Keyword(s):  
Ribosomal Rna ◽  
Ribonucleic Acid ◽  
Messenger Rna ◽  
Deoxyribonucleic Acid ◽  
Cellulose Nitrate ◽  
Absolute Size ◽  
Rna Molecules ◽  
Membrane Filters ◽  
Ribosomal Ribonucleic Acid

A simple and efficient method for hybridization and subsequent recovery of non-fragmented ribosomal RNA from the hybrid is described. The procedure involves annealing of immobilized denatured DNA bound on cellulose nitrate membrane filters to complementary RNA in 50% (v/v) formamide–0·33m-potassium chloride–10mm-tris–hydrochloric acid buffer, pH7·4, at 33° for 3hr. Under these conditions no detectable changes in the sedimentation coefficients of the input RNA were detected. The RNA can subsequently be recovered quantitatively from the hybrid in intact form by incubating the filters in formamide or in 85% (v/v) dimethyl sulphoxide. The applicability of the method for the evaluation of the absolute size of ribosomal RNA cistrons in Escherichia coli DNA and for the determination of the size of messenger RNA molecules is discussed.

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