scholarly journals Microsomal components in relation to amino acid incorporation by preparations from the developing rat brain

1967 ◽  
Vol 104 (1) ◽  
pp. 229-238 ◽  
Author(s):  
L Lim ◽  
DH Adams
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Developing Rat

Kainic acid inhibits protein amino acid incorporation in select rat brain regions

Neuroreport ◽  
1994 ◽  
Vol 5 (17) ◽  
pp. 2333-2336 ◽  
Author(s):  
Anna M. Planas ◽  
Marc A. Soriano ◽  
Isidre Ferrer ◽  
Eduard Rodríguez-Farré
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Kainic Acid ◽  
Brain Regions ◽  
Amino Acid Incorporation ◽  
Protein Amino Acid ◽  
Rat Brain Regions ◽  
Acid Incorporation

Amino Acid Incorporation into Myelin in Cell-Free Rat Brain Preparations

Science ◽  
1964 ◽  
Vol 144 (3618) ◽  
pp. 564-564
Author(s):  
C. B. Klee ◽  
L. Sokoloff
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

scholarly journals Messenger ribonucleic acid of cerebral nuclei

1967 ◽  
Vol 105 (3) ◽  
pp. 1111-1118 ◽  
Author(s):  
S. C. Bondy ◽  
Sidney Roberts
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Ribosomal Rna ◽  
Sodium Dodecyl ◽  
Significant Proportion ◽  
Amino Acid Incorporation ◽  
Messenger Ribonucleic Acid ◽  
Acid Incorporation ◽  
Rat Brain And Liver ◽  
Nuclear Rna

1. RNA was isolated from crude nuclear preparations and from ribosomes derived from rat brain and liver. Nuclear RNA was obtained by lysis of the nuclei with sodium dodecyl sulphate, followed by denaturation and removal of DNA and protein with hot phenol. 2. Base composition analyses indicated that the cerebral nuclear RNA preparation contained a higher proportion of non-ribosomal RNA than the analogous hepatic preparation. 3. Sucrose-density-gradient analyses revealed a heterogeneous profile for each nuclear RNA preparation, with two major peaks possessing the sedimentation properties of ribosomal RNA (18s and 28s). 4. Template activities of both preparations were widely distributed through the sucrose density gradients. 5. The cerebral nuclear RNA preparation was more active than the hepatic nuclear RNA preparation in promoting amino acid incorporation in cell-free systems from Escherichia coli and rat brain. 6. Cerebral nuclear RNA stimulated amino acid incorporation in a cerebral ribosomal system even in the presence of an excess of purified E. coli transfer RNA. 7. It is concluded that a significant proportion of cerebral nuclear RNA has the characteristics of messenger RNA.

Post-microsomal fractions from rat brain which stimulate ribosomal amino acid incorporation

1969 ◽  
Vol 47 (2) ◽  
pp. 75-78 ◽  
Author(s):  
M. R. V. Murthy
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Bond Formation ◽  
Peptide Bond ◽  
Sucrose Density Gradient ◽  
Peptide Bond Formation ◽  
Amino Acid Incorporation ◽  
Transfer Rnas ◽  
Complete Inactivation ◽  
Acid Incorporation

Post-microsomal fractions from rat brain were prepared which stimulated the synthesis of protein when endogenous mRNA was available and enhanced the synthesis of polyphenylalanine in the presence of poly-U and monomeric ribosomes. The amino-acid-activating (or transfer) enzymes did not appear to be responsible for the observed effect since the reaction mixture contained sufficient amounts of these enzymes to permit an optimum rate of amino acid incorporation. The effect was not solely due to the presence of messenger or amino acid transfer RNAs since the activity of the fractions was only partly reduced by prior treatment with ribonuclease. Heating led to complete inactivation of the fractions. Results from amino-acid-incorporation experiments and from sucrose density-gradient centrifugations indicate that the effect might be produced at two levels, one at the level of formation or preservation of polyribosomal structure and the other possibly at the level of peptide bond formation.

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