scholarly journals A gas-liquid-chromatographic procedure for separating a wide range of metabolites occurring in urine or tissue extracts

1966 ◽  
Vol 101 (3) ◽  
pp. 792-810 ◽  
Author(s):  
CE Dalgliesh ◽  
EC Horning ◽  
MG Horning ◽  
KL Knox ◽  
K Yarger
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Shikimic Acid ◽  
Methyl Esters ◽  
Peak Position ◽  
Gas Chromatography Mass Spectrometry ◽  
Tissue Extracts ◽  
Wide Range ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

1. A gas-liquid-chromatographic procedure is described which permits separation and identification on the same chromatogram of a wide range of substances occurring in urine or tissue extracts. The method uses hydrogen flame ionization, which detects organic compounds whether free or conjugated with no requirement for specific reactive groups. 2. For chromatography, carboxyl groups are quantitatively converted into methyl esters or trimethylsilyl esters. Phenolic, alcoholic and potential enolic groups are converted into trimethylsilyl ethers. Separations are carried out on a 6ft. column of either 10% F-60 (a polysiloxane) or 1% F-60, temperature programming at 2 degrees /min. being used over such part of the temperature range 30 degrees -260 degrees as is required. Propionyl derivatives of hydroxy compounds can also be used, but only on a non-quantitative basis. Derivatives and columns have been selected for optimum range of usefulness when large numbers of samples are examined by using automated gas chromatography. 3. The method is applicable to: fatty acids above butyric acid; di- and tri-carboxylic acids; hydroxy acids and keto acids; polyhydroxy and alicyclic compounds such as glycerol, inositol, quinic acid, shikimic acid, ascorbic acid and sugar alcohols; aromatic hydroxy and acidic compounds, both benzenoid and indolic; sesquiterpenes; steroids; glycine conjugates; mercapturic acids; glucuronides. It is not satisfactory for sulphate conjugates, iminazoles or polypeptides. 4. Methylene units provide an accurate and reproducible parameter for characterizing peak position. Methylene unit values are reported for a large variety of substances occurring in, or related to those occurring in, urine and tissue extracts. 5. The nature of derivatives was confirmed by combining gas chromatography with mass spectrometry. Combined gas chromatography-mass spectrometry gives a diagnostic tool of great power in the evaluation of metabolic patterns, and various uses are discussed.

scholarly journals Upon the opportunity to apply ART2 Neural Network for clusterization of biodiesel fuels

2016 ◽  
Vol 3 (1) ◽  
pp. 19-25
Author(s):  
T. Petkov ◽  
Z. Mustafa ◽  
S. Sotirov ◽  
R. Milina ◽  
M. Moskovkina
Keyword(s):  
Neural Network ◽  
Mass Spectrometry ◽  
Artificial Neural Network ◽  
Gas Chromatography ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Acid Methyl ◽  
Chemometric Approach ◽  
Biodiesel Fuels

Abstract A chemometric approach using artificial neural network for clusterization of biodiesels was developed. It is based on artificial ART2 neural network. Gas chromatography (GC) and Gas Chromatography - mass spectrometry (GC-MS) were used for quantitative and qualitative analysis of biodiesels, produced from different feedstocks, and FAME (fatty acid methyl esters) profiles were determined. Totally 96 analytical results for 7 different classes of biofuel plants: sunflower, rapeseed, corn, soybean, palm, peanut, “unknown” were used as objects. The analysis of biodiesels showed the content of five major FAME (C16:0, C18:0, C18:1, C18:2, C18:3) and those components were used like inputs in the model. After training with 6 samples, for which the origin was known, ANN was verified and tested with ninety “unknown” samples. The present research demonstrated the successful application of neural network for recognition of biodiesels according to their feedstock which give information upon their properties and handling.

scholarly journals Methodology for Studying Oxidation of Organic Species in Solution

2000 ◽  
Vol 83 (3) ◽  
pp. 584-587 ◽  
Author(s):  
Susan M Grimes ◽  
Helen C Ngwang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Inorganic Carbon ◽  
Photochemical Oxidation ◽  
Photocatalytic Decomposition ◽  
Gas Chromatography Mass Spectrometry ◽  
Uv Spectrophotometry ◽  
Total Inorganic Carbon ◽  
Organic Species ◽  
Wide Range

Abstract A photoreactor was developed to study products of photochemical oxidation in a wide range of organic compounds. Analysis of the products from the reactor were used to determine the extent of mineralization of the organic material, to characterize any intermediate compounds formed, and to obtain information on the decomposition mechanism. Appropriate methods for separation and characterization include LC, UV spectrophotometry, gas chromatography/mass spectrometry, total organic carbon, and total inorganic carbon. The uses of the reactor are described for the photocatalytic decomposition of phenol and of its major decomposition intermediates 1,2- and 1,4-dihydroxybenzene.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

Sign in / Sign up

Export Citation Format

Share Document