scholarly journals Separation of antigens by immunological specificity. Use of cellulose-linked antibodies as immunosorbents

1966 ◽  
Vol 101 (3) ◽  
pp. 711-716 ◽  
Author(s):  
RGC Gallop ◽  
BT Tozer ◽  
J Stephen ◽  
H Smith
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Gamma Globulin ◽  
High Specificity ◽  
Immunological Specificity

1. Immunosorbents were prepared by coupling activated aminocellulose with the gamma-globulin concentrates of antisera prepared against ovalbumin and human serum albumin. 2. The immunosorbents were low in solubility, but high in capacity for homologous antigens. 3. The high specificity of these immunosorbents was demonstrated by their use in fractionating various mixtures of fluorescent ovalbumin, (131)I-labelled human serum albumin, lysozyme and ribonuclease.

scholarly journals Separation of antigens by immunological specificity. Use of disulphide-linked antibodies as immunosorbents

1966 ◽  
Vol 101 (3) ◽  
pp. 717-720 ◽  
Author(s):  
J Stephen ◽  
RGC Gallop ◽  
H Smith
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Gamma Globulin ◽  
High Capacity ◽  
High Specificity ◽  
Homologous Antigen ◽  
Immunological Specificity ◽  
Insoluble Polymers ◽  
Low Solubility

1. gamma-Globulin concentrates of antisera prepared against ovalbumin and human serum albumin were thiolated and cross-linked to form insoluble polymers. 2. These immunosorbents were of low solubility and of high capacity for homologous antigen. 3. The high specificity of these immunosorbents was demonstrated by fractionation of various binary mixtures of fluorescent ovalbumin, (131)I-labelled human serum albumin, lysozyme and ribonuclease.

scholarly journals Separation of antigens by immunological specificity. Studies on the desorption of homologous antigen from disulphide-linked antibody immunosorbents

1970 ◽  
Vol 117 (1) ◽  
pp. 49-55 ◽  
Author(s):  
J. W. Chidlow ◽  
J. Stephen ◽  
H. Smith
Keyword(s):  
Hydrochloric Acid ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Acid Buffer ◽  
Homologous Antigen ◽  
Immunological Specificity

1. Glycine–hydrochloric acid buffer, pH2.2, desorbed 131I-labelled human serum albumin (100%), lysozyme (100%), ovalbumin (90%), fluorescent ovalbumin (50–60%) and fluorescent human γ-globulin (20%) from their respective homologous disulphide-linked antibody immunosorbents; reasons are suggested for the low recoveries of the fluorescently labelled proteins. 2. Approx. 40% of the recovered 131I-labelled human serum albumin and fluorescent ovalbumin was desorbed above pH6.0, but lysozyme was not eluted until the pH was 3.0 or below. 3. In all cases where high recoveries of antigen were obtained, the immunosorbents could be regenerated and recycled at least four times with full retention of specificity and minimal diminution of capacity. 4. The desorbed antigens were unchanged when compared with the original antigens by quantitative precipitin, specificradioactivity, fluorescent and enzymic analyses and by cellulose acetate electrophoresis. 5. Desorption of antigen with a variety of reagents was investigated. These reagents were less satisfactory than glycine–hydrochloric acid.

High Efficiency Photolabeling of Human Serum Albumin and Human .gamma.-Globulin with [14C]Methyl 4-Azido-2,3,5,6-tetrafluorobenzoate

1995 ◽  
Vol 6 (5) ◽  
pp. 630-634 ◽  
Author(s):  
Raghoottama S. Pandurangi ◽  
Srinivasa R. Karra ◽  
Robert R. Kuntz ◽  
Wynn A. Volkert
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
High Efficiency ◽  
Gamma Globulin ◽  
Human Gamma Globulin

Changes in Protein Adsorption on Self-Assembled Monolayers with Monolayer Order:  Comparison of Human Serum Albumin and Human Gamma Globulin

Langmuir ◽  
2001 ◽  
Vol 17 (24) ◽  
pp. 7645-7651 ◽  
Author(s):  
Stanislaw Petrash ◽  
Tricia Cregger ◽  
Bin Zhao ◽  
Elena Pokidysheva ◽  
Mark D. Foster ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Adsorption ◽  
Gamma Globulin ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Human Gamma Globulin ◽  
Order Comparison

Synthesis of conjugates of 5'-O-carboxymethyl-5-halogeno-2'-deoxyuridines and 5'-O-carboxymethyl-5-halogenouridines with proteins

1980 ◽  
Vol 45 (2) ◽  
pp. 606-610 ◽  
Author(s):  
Helmut Pischel ◽  
Antonín Holý ◽  
Günther Wagner
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Gamma Globulin ◽  
Isobutyl Chloroformate ◽  
Mixed Anhydrides ◽  
Covalently Bound

5'-O-Carboxymethyl-2'-deoxyuridine (Ia), its 5-fluoro, 5-bromo and 5-iodo derivatives Ib-Id, and 5'-O-carboxymethyluridine (IIa) and its 5-halogeno derivatives IIb-IId, on reaction with isobutyl chloroformate and tri-n-butylamine afforded mixed anhydrides IIIa-IIId and IVa-IVd. Condensation of III and IV with human serum albumin or bovine gamma-globulin at pH 8.0 gave conjugates Va-Vd to VIIIa-VIIId, the yield of the covalently bound haptene being 28 to 43%.

II. Lokalisation der Placenta mit 113Inm- Human-Serum-Albumin

1969 ◽  
Vol 08 (01) ◽  
pp. 15-21 ◽  
Author(s):  
K. E. Scheer ◽  
J. Heep ◽  
W. Maier-Borst ◽  
W. J. Lorenz ◽  
H. Sinn ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Vena Cava ◽  
Placenta Praevia ◽  
Wird Eine

ZusammenfassungNach tierexperimentellen Voruntersuchungen wurde die Placentographie mit trägerfreiem 113Inm -HSA als klinische Methode eingeführt. Vor Amniocentesen und bei Verdacht auf Placenta praevia werden Placentographien geschrieben. Den Schwangeren wird eine Aktivität von 500 μCi in die Cubitalvene injiziert. Die der Aktivität entsprechende Indiummenge ist kleiner als 0,1 ng. Die fetale Strahlenbelastung liegt unter lOmrad. Bei Anwendung von 113Inm-HSA entfällt eine Blockade der mütterlichen und fetalen Schilddrüsen. Die genaue Abgrenzung einer Placenta praevia wird nicht durch eine Blasenaktivität beeinträchtigt.Es wurden bisher 19 Placentalokalisationen durchgeführt. In allen Fällen konnte der Placentasitz eindeutig festgestellt werden. Bedingt durch die lange Liegezeit beim Aufnehmen eines Szintigramms kam es in zwei Fällen zu einem Vena-Cava-Kompressions-Syndrom. Zur Verhinderung dieser klinischen Zwischenfälle werden inzwischen Placentographien mit der Anger-Kamera aufgenommen. Mit Hilfe des divergierenden Kollimators konnte der gesamte Abdominalbereich erfaßt werden. Die Aufnahmezeit konnte auf 7 — 10 Minuten verkürzt werden. Die intravenöse injizierte Aktivität betrug bei dieser Methode ebenfalls 500 μCi. Der diagnostische Aussagewert der Kamerabilder ist szintigraphischen Aufnahmen gleichwertig.

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