scholarly journals The active centre of triose phosphate isomerase

1966 ◽  
Vol 100 (3) ◽  
pp. 702-710 ◽  
Author(s):  
PM Burton ◽  
SG Waley
Keyword(s):  
Triose Phosphate Isomerase ◽  
Active Centre ◽  
Triose Phosphate

scholarly journals The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate

1971 ◽  
Vol 123 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Janet C. Miller ◽  
S. G. Waley
Keyword(s):  
Phosphoric Acid ◽  
Glutamic Acid ◽  
Inorganic Phosphate ◽  
Triose Phosphate Isomerase ◽  
Rabbit Muscle ◽  
Irreversible Inhibitor ◽  
Active Centre ◽  
Triose Phosphate ◽  
Glutamic Acid Residue

1. Glycidol (2,3-epoxypropanol) phosphate is a specific irreversible inhibitor of rabbit muscle triose phosphate isomerase (EC 5.3.1.1); the site of attachment has now been studied. 2. The labelled enzyme was digested with pepsin and a modified peptide isolated. The sequence of the peptide is: Ala-Tyr-Glu-Pro-Val-Trp. 3. It is the glutamic acid residue in this peptide that is labelled: the peptide is thus a γ-glutamyl ester derived from glycerol phosphoric acid. The same site is labelled by a mixture of glycidol and inorganic phosphate. 4. Kinetic and stereochemical features of these reactions are discussed.

scholarly journals A High-Content Screening Assay for Small Molecules That Stabilize Mutant Triose Phosphate Isomerase (TPI) as Treatments for TPI Deficiency

2021 ◽  
pp. 247255522110181
Author(s):  
Andreas Vogt ◽  
Samantha L. Eicher ◽  
Tracey D. Myers ◽  
Stacy L. Hrizo ◽  
Laura L. Vollmer ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Large Scale ◽  
Protein Quality ◽  
Fluorescent Protein ◽  
Triose Phosphate Isomerase ◽  
Pharmacological Intervention ◽  
Screening Assay ◽  
Triose Phosphate ◽  
The Common ◽  
Green Fluorescent

Triose phosphate isomerase deficiency (TPI Df) is an untreatable, childhood-onset glycolytic enzymopathy. Patients typically present with frequent infections, anemia, and muscle weakness that quickly progresses with severe neuromusclar dysfunction requiring aided mobility and often respiratory support. Life expectancy after diagnosis is typically ~5 years. There are several described pathogenic mutations that encode functional proteins; however, these proteins, which include the protein resulting from the “common” TPIE105D mutation, are unstable due to active degradation by protein quality control (PQC) pathways. Previous work has shown that elevating mutant TPI levels by genetic or pharmacological intervention can ameliorate symptoms of TPI Df in fruit flies. To identify compounds that increase levels of mutant TPI, we have developed a human embryonic kidney (HEK) stable knock-in model expressing the common TPI Df protein fused with green fluorescent protein (HEK TPIE105D-GFP). To directly address the need for lead TPI Df therapeutics, these cells were developed into an optical drug discovery platform that was implemented for high-throughput screening (HTS) and validated in 3-day variability tests, meeting HTS standards. We initially used this assay to screen the 446-member National Institutes of Health (NIH) Clinical Collection and validated two of the hits in dose–response, by limited structure–activity relationship studies with a small number of analogs, and in an orthogonal, non-optical assay in patient fibroblasts. The data form the basis for a large-scale phenotypic screening effort to discover compounds that stabilize TPI as treatments for this devastating childhood disease.

scholarly journals Triose Phosphate Isomerase from Bakers' Yeast

1972 ◽  
Vol 247 (10) ◽  
pp. 3361-3362
Author(s):  
Stuart W. Hawkinson ◽  
Chin Hsuan Wei ◽  
Fred C. Hartman ◽  
I. Lucille Norton ◽  
J. Ralph Einstein
Keyword(s):  
Triose Phosphate Isomerase ◽  
Triose Phosphate

PRENATAL DIAGNOSIS OF TRIOSE PHOSPHATE ISOMERASE DEFICIENCY

The Lancet ◽  
1989 ◽  
Vol 334 (8667) ◽  
pp. 871 ◽  
Author(s):  
B. Dallapiccola ◽  
G. Novelli
Keyword(s):  
Prenatal Diagnosis ◽  
Triose Phosphate Isomerase ◽  
Triose Phosphate

On the three-dimensional structure and catalytic mechanism of triose phosphate isomerase

1981 ◽  
Vol 293 (1063) ◽  
pp. 159-171 ◽  
Keyword(s):  
Catalytic Mechanism ◽  
Polypeptide Chain ◽  
Three Dimensional ◽  
Triose Phosphate Isomerase ◽  
Dimensional Structure ◽  
Dihydroxyacetone Phosphate ◽  
Triose Phosphate ◽  
Independent Determination ◽  
Chicken Muscle

Triose phosphate isomerase is a dimeric enzyme of molecular mass 56000 which catalyses the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate. The crystal structure of the enzyme from chicken muscle has been determined at a resolution of 2.5 A, and an independent determination of the structure of the yeast enzyme has just been completed at 3 A resolution. The conformation of the polypeptide chain is essentially identical in the two structures, and consists of an inner cylinder of eight strands of parallel |3-pleated sheet, with mostly helical segments connecting each strand. The active site is a pocket containing glutamic acid 165, which is believed to act as a base in the reaction. Crystallographic studies of the binding of DHAP to both the chicken and the yeast enzymes reveal a common mode of binding and suggest a mechanism for catalysis involving polarization of the substrate carbonyl group.

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