scholarly journals Magnesium ions and the structure of Escherichia coli ribosomal ribonucleic acid

1966 ◽  
Vol 100 (1) ◽  
pp. 102-109 ◽  
Author(s):  
A Rodgers
Keyword(s):  
Escherichia Coli ◽  
Ribonucleic Acid ◽  
Magnesium Ions ◽  
Ribosomal Ribonucleic Acid

scholarly journals A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes

1976 ◽  
Vol 160 (3) ◽  
pp. 505-519 ◽  
Author(s):  
R A Cox ◽  
W Hirst
Keyword(s):  
Escherichia Coli ◽  
Secondary Structure ◽  
Ribonucleic Acid ◽  
Magnesium Ions ◽  
Base Pairs ◽  
E Coli ◽  
Ribosomal Ribonucleic Acid ◽  
The Stability ◽  
Rabbit Reticulocytes

Mg2+ was shown to affect the conformation of rRNA over the range of 0.03-1.2M-KCl. The species studies were Escherichia coli S-rRNA and L-rRNA (the RNA moieties of the smaller and larger subribosomal particles respectively) and rabbits S-rRNA and L-rRNA. 2. The addition of Mg2+ to rRNA in reconstitution buffer (0.35M-KCl0.01M-Tris/HCl, pH7.2) at 20° C let to an increase in bihelical secondary structure through the formation of additional (mainly A-U) base-pairs (e.g. an additional approx. 58 A-U base-pairs per molecule of E. coli S-rRNA as judged by u.v. difference spectrophotometry…

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