scholarly journals The glycerol teichoic acid from the cell wall of Bacillus stearothermophilus B65

1966 ◽  
Vol 99 (1) ◽  
pp. 108-116 ◽  
Author(s):  
AJ Wicken
Keyword(s):  
Cell Wall ◽  
Bacillus Stearothermophilus ◽  
Teichoic Acid

scholarly journals A Staphylococcus aureus clpX Mutant Used as a Unique Screening Tool to Identify Cell Wall Synthesis Inhibitors that Reverse β-Lactam Resistance in MRSA

2021 ◽  
Vol 8 ◽  
Author(s):  
Kristoffer T. Bæk ◽  
Camilla Jensen ◽  
Maya A. Farha ◽  
Tobias K. Nielsen ◽  
Ervin Paknejadi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
High Throughput Screening ◽  
Bacterial Infections ◽  
Screening Tool ◽  
Acquired Resistance ◽  
Teichoic Acid ◽  
Acid Synthesis ◽  
Biologically Active ◽  
Cell Wall Synthesis

Staphylococcus aureus is a leading cause of bacterial infections world-wide. Staphylococcal infections are preferentially treated with β-lactam antibiotics, however, methicillin-resistant S. aureus (MRSA) strains have acquired resistance to this superior class of antibiotics. We have developed a growth-based, high-throughput screening approach that directly identifies cell wall synthesis inhibitors capable of reversing β-lactam resistance in MRSA. The screen is based on the finding that S. aureus mutants lacking the ClpX chaperone grow very poorly at 30°C unless specific steps in teichoic acid synthesis or penicillin binding protein (PBP) activity are inhibited. This property allowed us to exploit the S. aureus clpX mutant as a unique screening tool to rapidly identify biologically active compounds that target cell wall synthesis. We tested a library of ∼50,000 small chemical compounds and searched for compounds that inhibited growth of the wild type while stimulating growth of the clpX mutant. Fifty-eight compounds met these screening criteria, and preliminary tests of 10 compounds identified seven compounds that reverse β-lactam resistance of MRSA as expected for inhibitors of teichoic acid synthesis. The hit compounds are therefore promising candidates for further development as novel combination agents to restore β-lactam efficacy against MRSA.

scholarly journals Characterization of a Bacillus subtilis Thermosensitive Teichoic Acid-Deficient Mutant: Gene mnaA (yvyH) Encodes the UDP-N-Acetylglucosamine 2-Epimerase

2002 ◽  
Vol 184 (15) ◽  
pp. 4316-4320 ◽  
Author(s):  
Blazenka Soldo ◽  
Vladimir Lazarevic ◽  
Harold M. Pooley ◽  
Dimitri Karamata
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Cell Morphology ◽  
Teichoic Acid ◽  
Mutant Gene ◽  
Deficient Mutant ◽  
Phosphate Content ◽  
Nonpermissive Temperature ◽  
Coccoid Cell

ABSTRACT The Bacillus subtilis thermosensitive mutant ts-21 bears two C-G→T-A transitions in the mnaA gene. At the nonpermissive temperature it is characterized by coccoid cell morphology and reduced cell wall phosphate content. MnaA converts UDP-N-acetylglucosamine into UDP-N-acetylmannosamine, a precursor of the teichoic acid linkage unit.

scholarly journals Glycosylation of Staphylococcus aureus cell wall teichoic acid is influenced by environmental conditions

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Noëlle Mistretta ◽  
Marina Brossaud ◽  
Fabienne Telles ◽  
Violette Sanchez ◽  
Philippe Talaga ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Environmental Conditions ◽  
Teichoic Acid ◽  
Wall Teichoic Acid

A novel type of teichoic acid from the cell wall of Bacillus subtilis VKM B-762

2011 ◽  
Vol 346 (9) ◽  
pp. 1173-1177 ◽  
Author(s):  
Alexander S. Shashkov ◽  
Galina M. Streshinskaya ◽  
Yuliya I. Kozlova ◽  
Sof’ya N. Senchenkova ◽  
Nikolay P. Arbatsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacillus Subtilis ◽  
Teichoic Acid

Structure of the cell wall of Staphylococcus aureus. IX. Teichoic acid and phage adsorption

Biochemistry ◽  
1968 ◽  
Vol 7 (6) ◽  
pp. 2385-2389 ◽  
Author(s):  
Jacques. Coyette ◽  
Jean Marie. Ghuysen
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Teichoic Acid ◽  
Phage Adsorption
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