scholarly journals The distribution of free amino acids in subcellular fractions of guinea-pig brain

1966 ◽  
Vol 98 (1) ◽  
pp. 128-137 ◽  
Author(s):  
JL Mangan ◽  
VP Whittaker
Keyword(s):  
Amino Acids ◽  
Guinea Pig ◽  
Free Amino Acids ◽  
Free Amino ◽  
Subcellular Fractions ◽  
Pig Brain ◽  
Guinea Pig Brain

Free amino acids of newborn and adult guinea pig brain

1968 ◽  
Vol 1 (1) ◽  
pp. 24-29 ◽  
Author(s):  
Jimmie M. Davis ◽  
Williamina A. Himwich ◽  
Harish C. Agrawal
Keyword(s):  
Amino Acids ◽  
Guinea Pig ◽  
Free Amino Acids ◽  
Free Amino ◽  
Pig Brain ◽  
Guinea Pig Brain

scholarly journals The subcellular distribution of triphosphoinositide phosphomonoesterase in guinea-pig brain

1972 ◽  
Vol 128 (3) ◽  
pp. 579-586 ◽  
Author(s):  
A. Sheltawy ◽  
M. Brammer ◽  
D. Borrill
Keyword(s):  
Guinea Pig ◽  
Subcellular Fractions ◽  
Assay System ◽  
Acid Phosphatases ◽  
Alkaline Phosphatases ◽  
Pig Brain ◽  
Optimum Ph ◽  
Ph Range ◽  
Guinea Pig Brain ◽  
Optimum Ph Range

1. Some properties of the triphosphoinositide phosphomonoesterase from the homogenates of guinea-pig brain were studied. The enzyme has an optimum pH range 6.7–7.3, is stimulated with KCl at a concentration of 0.1m, and under these conditions has Km1.43×10-4m. 2. A factor from the ‘pH5 supernatant’ of guinea-pig brain stimulates the enzyme activity over and above the stimulation produced by KCl. Subcellular fractions of guinea-pig brain varied in their response to the ‘pH5 supernatant’. Maximum stimulation was observed with the P1 fraction, containing myelin and nuclei. 3. An assay system for the enzyme was developed that contained optimum concentrations of both KCl and the ‘pH5 supernatant’. Acid phosphatases were inhibited by NaF, but, in contrast with previous work, no EDTA was added to the assay system to inhibit the alkaline phosphatases. This reagent inhibited the triphosphoinositide phosphomonoesterase. It was estimated that the remaining fraction of non-specific phosphatases can account for only 14% of the observed triphosphoinositide phosphomonoesterase activity. 4. Subcellular fractions of guinea-pig brain were characterized by electron microscopy and subcellular markers. The triphosphoinositide phosphomonoesterase exhibited a distribution between the fractions similar to that of 5′-nucleotidase, but different from that of alkaline phosphatase.

scholarly journals Free amino acids of testes. Concentrations of free amino acids in the testes of several species and the precursors of glutamate and glutamine in rat testes in vivo

1973 ◽  
Vol 132 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Isa K. Mushahwar ◽  
Roger E. Koeppe
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Guinea Pig ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ammonium Acetate ◽  
Testicular Tissue ◽  
Species Variation

Determination of the free amino acid and lactate content of testicular tissue in rat, guinea pig, rabbit, cat, gerbil, hamster, chicken and bullfrog indicates a substantial species variation. Insulin hypoglycaemia and ammonium acetate toxicity changes the concentration of several free amino acids of rat testes. 14C radioactivity from labelled acetate and ethanol is rapidly incorporated into some of the free amino acids of rat testes in vivo, whereas incorporation from [14C]glucose is relatively slow. These results have been compared with those obtained from similar studies with rat brain. In contrast to brain, there is no evidence for glutamate compartmentation in testes.

ENZYME ACTIVITIES OF THE SUBCELLULAR FRACTIONS OF MYELIN IN THE DEVELOPING GUINEA-PIG BRAIN

2009 ◽  
Vol 46 (S43) ◽  
pp. 231-231
Author(s):  
H. Frey ◽  
P. Riekkinen ◽  
A. Arstila ◽  
U. K. Rinne
Keyword(s):  
Guinea Pig ◽  
Enzyme Activities ◽  
Subcellular Fractions ◽  
Pig Brain ◽  
Guinea Pig Brain
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