scholarly journals The effect of vitamin A deficiency and dietary α-tocopherol on the stability of rat-liver lysosomes

1965 ◽  
Vol 97 (2) ◽  
pp. 353-359 ◽  
Author(s):  
OA Roels ◽  
M Trout ◽  
A Guha
Keyword(s):  
Vitamin A ◽  
Vitamin A Deficiency ◽  
Enzymic Activity ◽  
Alpha Tocopherol ◽  
Enzyme Release ◽  
Normal Amount ◽  
Prolonged Incubation ◽  
Liver Lysosomes ◽  
The Stability ◽  
Rat Liver Lysosomes

1. Vitamin A-deficient rats and pair-fed controls were maintained on either normal or raised amounts of dietary alpha-tocopherol. 2. Their livers were fractionated and; free' and; total' lysosomal phosphatase were determined in the various fractions. The rate of release of this enzyme was determined in the mitochondria-lysosome-rich fraction during incubation at pH5 and 37 degrees . 3. The deficient livers showed increased enzymic activity. 4. Prolonged incubation caused more rapid enzyme release from the mitochondria-lysosome-rich fraction of the vitamin A-deficient rats receiving the normal amount of dietary alpha-tocopherol than from the equivalent fraction of their pair-fed controls receiving vitamin A. Raised dietary alpha-tocopherol reversed this phenomenon.

scholarly journals Aflatoxin B1 albumin adducts in plasma and aflatoxin M1 in urine are associated with plasma concentrations of vitamins A and E

2010 ◽  
Vol 80 (6) ◽  
pp. 355-368 ◽  
Author(s):  
Francis A. Obuseh ◽  
Pauline E. Jolly ◽  
Yi Jiang ◽  
Faisal M.B. Shuaib ◽  
John Waterbor ◽  
...  
Keyword(s):  
Vitamin E ◽  
Vitamin A ◽  
Vitamin A Deficiency ◽  
Plasma Concentrations ◽  
Multivariable Analysis ◽  
Alpha Tocopherol ◽  
Aflatoxin M1 ◽  
Vitamin E Deficiency ◽  
Cross Sectional ◽  
Virus Surface

Background: Although aflatoxin exposure has been associated with micronutrient deficiency in animals, there are few investigations on the effects of aflatoxin exposure on micronutrient metabolism in humans. Objective: To examine the relationship between aflatoxin B1 (AFB1) albumin adducts (AF-ALB) in plasma and the aflatoxin M1 (AFM1) metabolite in urine and plasma concentrations of retinol (vitamin A) and alpha-tocopherol (vitamin E) in Ghanaians. Methods: A cross-sectional study of 147 adult participants was conducted. Blood and urine samples were tested for aflatoxin and vitamins A and E levels. Results: Multivariable analysis showed that participants with high AF-ALB (>= 0.80 pmol/mg albumin) had increased odds of having vitamin A deficiency compared to those with lower AF-ALB [Odds Ratio (OR) = 2.61; CI = 1.03 - 6.58; p = 0.04]. Participants with high AF-ALB also showed increased odds of having vitamin E deficiency but this was not statistically significant (OR = 2.4; CI = 0.96 - 6.05; p = 0.06). Conversely, those with higher AFM1 values had a statistically nonsignificant reduced odds of having vitamin A deficiency (OR = 0.31; CI = 0.09 - 1.02; p = 0.05) and a statistically significant reduced odds of having vitamin E deficiency (OR = 0.31; CI = 0.10 - 0.97; p = 0.04). Participants with high AF-ALB or high AFM1 (>= 437.95 pg/dL creatinine) were almost 6 times more likely to be hepatitis B virus surface antigen (HBsAg)-positive (OR = 5.88; CI = 1.71 - 20.14; p = 0.005) and (OR = 5.84; CI = 1.15 - 29.54; p = 0.03) respectively. Conclusions: These data indicate that aflatoxin may modify plasma micronutrient status. Thus, preventing aflatoxin exposure may reduce vitamin A and E deficiencies.

scholarly journals Effect of polar radiation degradation products of .BETA.-carotene and vitamin A on rat liver lysosomes.

1977 ◽  
Vol 41 (1) ◽  
pp. 125-130 ◽  
Author(s):  
Brit BHUSHAN ◽  
P. HARIKUMAR ◽  
S. B. K. WARRIER ◽  
V. NINJOOR ◽  
U. S. KUMTA
Keyword(s):  
Vitamin A ◽  
Rat Liver ◽  
Degradation Products ◽  
Beta Carotene ◽  
Radiation Degradation ◽  
Liver Lysosomes ◽  
Rat Liver Lysosomes

scholarly journals Effect of Polar Radiation Degradation Products ofβ-Carotene and Vitamin A on Rat Liver Lysosomes

1977 ◽  
Vol 41 (1) ◽  
pp. 125-130
Author(s):  
Brij Bhushan ◽  
P. Harikumar ◽  
S. B. K. Warrier ◽  
V. Ninjoor ◽  
U. S. Kumta
Keyword(s):  
Vitamin A ◽  
Rat Liver ◽  
Degradation Products ◽  
Radiation Degradation ◽  
Liver Lysosomes ◽  
Rat Liver Lysosomes

Quantification of Vitamin A in Fortified Rapeseed, Groundnut and Soya Oils Using a Simple Portable Device: Comparison to High Performance Liquid Chromatography

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Developed Countries ◽  
Portable Device ◽  
Least Developed Countries ◽  
Control Food ◽  
Assay Precision

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.

Research Recommendations for Applying Vitamin A-Labelled Isotope Dilution Techniques to Improve Human Vitamin A Nutrition

2014 ◽  
Vol 84 (Supplement 1) ◽  
pp. 52-59 ◽  
Author(s):  
Sherry A. Tanumihardjo ◽  
Anura V. Kurpad ◽  
Janet R. Hunt
Keyword(s):  
Public Health ◽  
Vitamin A ◽  
Vitamin A Deficiency ◽  
Human Subjects ◽  
The Body ◽  
Pool Size ◽  
Serum Retinol ◽  
Vitamin A Status ◽  
Status Assessment ◽  
Total Body

The current use of serum retinol concentrations as a measurement of subclinical vitamin A deficiency is unsatisfactory for many reasons. The best technique available for vitamin A status assessment in humans is the measurement of total body pool size. Pool size is measured by the administration of retinol labelled with stable isotopes of carbon or hydrogen that are safe for human subjects, with subsequent measurement of the dilution of the labelled retinol within the body pool. However, the isotope techniques are time-consuming, technically challenging, and relatively expensive. There is also a need to assess different types of tracers and doses, and to establish clear guidelines for the use and interpretation of this method in different populations. Field-friendly improvements are desirable to encourage the application of this technique in developing countries where the need is greatest for monitoring the risk of vitamin A deficiency, the effectiveness of public health interventions, and the potential of hypervitaminosis due to combined supplement and fortification programs. These techniques should be applied to validate other less technical methods of assessing vitamin A deficiency. Another area of public health relevance for this technique is to understand the bioconversion of β-carotene to vitamin A, and its relation to existing vitamin A status, for future dietary diversification programs.

Vitamin A deficiency is associated with disease severity in patients with liver cirrhosis

2020 ◽  
Author(s):  
M Nagel ◽  
C Labenz ◽  
M Nguyen-Tat ◽  
N Cabezas Wallscheid ◽  
C Czauderna ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Vitamin A ◽  
Disease Severity ◽  
Vitamin A Deficiency
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