scholarly journals THE BIOSYNTHESIS OF GRAMICIDIN S IN A CELL-FREE SYSTEM

1965 ◽  
Vol 96 (1) ◽  
pp. 43-52 ◽  
Author(s):  
TL BERG ◽  
LO FROHOLM ◽  
SG LALAND
Keyword(s):  
Free System ◽  
Cell Free System ◽  
Gramicidin S ◽  
A Cell

Biosynthesis of gramicidin S by a cell-free system of bacillus brevis

1965 ◽  
Vol 19 (2) ◽  
pp. 204-208 ◽  
Author(s):  
Munehiko Yukioka ◽  
Yuso Tsukamoto ◽  
Yoshitaka Saito ◽  
Takitaro Tsuji ◽  
Shuzo Otani ◽  
...  
Keyword(s):  
Bacillus Brevis ◽  
Free System ◽  
Cell Free System ◽  
Gramicidin S ◽  
A Cell

scholarly journals Studies on the biosynthesis of gramicidin S in a cell-free system from Bacillus brevis. Further attempts to elucidate its mechanism of synthesis

1967 ◽  
Vol 105 (2) ◽  
pp. 451-453 ◽  
Author(s):  
K. J. Figenschou ◽  
L. O. Frøholm ◽  
S. G. Laland
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Cell Free Extract ◽  
Bacillus Brevis ◽  
Free System ◽  
Cell Free System ◽  
Gramicidin S ◽  
A Cell

1. The pH optima for the incorporation of 14C-labelled amino acids into gramicidin S by an 11000g cell-free extract from Bacillus brevis have been determined. The pH optima for leucine, proline, phenylalanine, ornithine and valine were 7·5–7·7, 7·5–7·7, 7·7–7·9, 7·7–7·9 and 8·0–8·2 respectively. Hence the greatest difference in pH optima existed between leucine and valine, where it was 0·5pH unit. 2. The 11000g cell-free extract incorporated into gramicidin S only the l-isomers of valine, proline and ornithine. However, both isomers of leucine are utilized and the experiments indicate that a leucine racemase exists in the 11000g cell-free extract. With phenylalanine the l-isomer is utilized much more effectively than the d-isomer. This is noteworthy since it is the d-isomer that occurs in gramicidin S. The experiments indicate that conversion of the l-isomer into the d-form takes place at a stage beyond that of the free amino acid.

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell
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