scholarly journals The Cytochemical Localization of Alkaline Phosphatase in Escherichia coli at the Electron-Microscope Level

1965 ◽  
Vol 96 (3) ◽  
pp. 27C-28C ◽  
Author(s):  
J. Done ◽  
C. D. Shorey, ◽  
Joan P. Loke ◽  
J. K. Pollak
Keyword(s):  
Escherichia Coli ◽  
Alkaline Phosphatase ◽  
Electron Microscope ◽  
Microscope Level ◽  
Electron Microscope Level ◽  
Cytochemical Localization

Sequential Changes Associated with Cell Wall Formation and Fusion in the Vascular Cambium

IAWA Journal ◽  
1981 ◽  
Vol 2 (4) ◽  
pp. 151-162 ◽  
Author(s):  
A.M. Catesson ◽  
J.C. Roland
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Cell Walls ◽  
Microscope Level ◽  
Cell Wall Formation ◽  
Characteristic Sequence ◽  
Electron Microscope Level ◽  
Starting Point ◽  
Cell Wall Development ◽  
Cambial Zone

Cytochemical techniques and mild extractions were used at the electron microscope level for the study of the cambial zone of several hardwoods and one softwood. The maturation processes of the primary radial and tangential cell walls involve a progressive disappearance of their initial heterogeneity. The buttress-like zone joining these walls appears to be the starting point for a characteristic sequence of changes and intra-wall rearrangement. Topochemical results have suggested an alternative to the 'emboxing concept' of cell wall development.

scholarly journals CYTOCHEMISTRY OF 5-HYDROXYTRYPTAMINE AT THE ELECTRON MICROSCOPE LEVEL I. STUDY OF THE SPECIFICITY OF THE REACTION IN ISOLATED BLOOD PLATELETS

1968 ◽  
Vol 16 (3) ◽  
pp. 162-171 ◽  
Author(s):  
GUILLERMO JAIM ETCHEVERRY ◽  
LUIS MARIA ZIEHER
Keyword(s):  
Electron Microscope ◽  
Blood Platelets ◽  
Microscope Level ◽  
Storage Site ◽  
Electron Microscope Level ◽  
Dense Granules ◽  
Amine Content ◽  
Level I ◽  
Previous Assumption

Blood platelets obtained from normal rabbits and those isolated from reserpine-treated animals and subsequently incubated in vitro with 5-hydroxytryptamine, norepinephrine and histamine were assayed for amine content or processed for examination under the electron microscope. With the glutaraldehyde-dichromate reaction for unsubstituted catechol- and indoleamines, reactive granules were observed in normal platelets. Formaldehyde fixation prior to the glutaraldehyde-dichromate reaction resulted in a similar image under the electron microscope. In platelets obtained from animals treated with reserpine a decrease of the amine content with a corresponding reduction in the number of dense granules was observed. Following incubation with 5-hydroxytryptamine the concentration of the amine increased markedly and the number of dense granules that reacted with both techniques became practically normal. In norepinephrine-incubated platelets dense granules were demonstrated with the glutaraldehyde-dichromate reaction, but no reactive products were observed using prefixation with formaldehyde. Histamine was also incorporated into depleted platelets but gave no reaction. It is concluded that prefixation with formaldehyde renders negative the reaction with catecholamines, leaving unaffected indoleamine-reactive sites. The previous assumption that the dense granules contain 5-hydroxytryptamine has been confirmed by such a cytochemical approach. The possibility that these organelles constitute a common storage site for different amines is discussed.

Infundibulomyces: a new genus of coelomycetes from Thailand

2003 ◽  
Vol 81 (7) ◽  
pp. 732-737 ◽  
Author(s):  
Narumol Plaingam ◽  
Sayanh Somrithipol ◽  
E B. Gareth Jones
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Type Species ◽  
New Genus ◽  
Anamorphic Fungi ◽  
Microscope Level ◽  
Electron Microscope Level ◽  
Fallen Leaves ◽  
Scanning Electron

The genus Infundibulomyces is proposed for coelomycetous fungi with nidulariaceous-like conidiomata and bipolar appendaged conidia. Infundibulomyces cupulata sp. nov., collected from fallen leaves of Lagerstroemia sp. from Thailand, is described as the type species of the genus. This fungus is illustrated at the light microscope and scanning electron microscope level and compared with other morphologically related taxa. Cupulate conidiomata are uncommon and Infundibulomyces is compared with these.Key words: anamorphic fungi, coelomycete, Infundibulomyces, Thailand, tropical.

scholarly journals Cytochemical localization of alkaline phosphatase and Na+-pump sites in adult rat colon.

1979 ◽  
Vol 27 (9) ◽  
pp. 1231-1235 ◽  
Author(s):  
P B Vengesa ◽  
U Hopfer
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Electron Microscope ◽  
Colonic Mucosa ◽  
Brush Border Membrane ◽  
Rat Colon ◽  
Adult Rats ◽  
Cytochemical Localization ◽  
Chief Cells ◽  
Adult Rat

The cellular and subcellular locialization of alkaline and K+-dependent phosphatase activities in the colonic mucosa of adult rats and rabbits was studied with the electron microscope. The 1-cysteine-sensitive alkaline phosphatase activity was observed in the brush border membrane of the chief cells. The contraluminal plasma membrane of chief cells was devoid of this enzyme activity. In contrast, the cardiac glycoside-sensitive K+-dependent phosphatase was predominantly localized in this region of the cheif cells.

ELECTRON MICROSCOPY OF ALKALINE PHOSPHATASE OF ESCHERICHIA COLI

1966 ◽  
Vol 12 (4) ◽  
pp. 605-607 ◽  
Author(s):  
V. M. Kushnarev ◽  
T. A. Smirnova
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Alkaline Phosphatase ◽  
Cell Wall ◽  
Enzyme Activity ◽  
Electron Microscope ◽  
Inorganic Phosphate ◽  
E Coli

A method is described for determining the localization of alkaline phosphatase in the cells of E. coli B with the electron microscope. Enzyme activity, determined by deposition of inorganic phosphate, is located in the exterior layer of the cell wall.

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