scholarly journals TERMINAL-SEQUENCE STUDIES OF HIGH-MOLECULAR-WEIGHT RIBONUCLEIC. THE REACTION OF PERIODATE-OXIDIZED RIBONUCLEOSIDES, 5′-RIBONUCLEOTIDES AND RIBONUCLEIC ACID WITH ISONIAZID

1965 ◽  
Vol 95 (2) ◽  
pp. 541-551 ◽  
Author(s):  
JA HUNT
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Terminal Sequence

scholarly journals Terminal sequence studies of high-molecular-weight ribonucleic acid. The 3′-termini of rabbit globin messenger ribonucleic acid

1973 ◽  
Vol 131 (2) ◽  
pp. 315-325 ◽  
Author(s):  
J. A. Hunt
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Periodate Oxidation ◽  
Apparent Molecular Weight ◽  
Paper Electrophoresis ◽  
Terminal Sequence ◽  
Globin Mrna ◽  
Messenger Ribonucleic Acid ◽  
Oxidation Analysis

Haemoglobin mRNA isolated from EDTA-treated polyribosomes has an apparent molecular weight of 120000–180000 estimated by condensation with 3H-labelled isoniazid after periodate oxidation. Analysis of the ribonuclease digests of isoniazid-labelled RNA by paper electrophoresis and column chromatography enables the amount of contaminating 18S, 7S, 5S and 4S RNA to be estimated, and a corrected molecular weight of globin mRNA as the acid is 161000 or 500 nucleotides in length. This molecule contains two groups of 3′-terminal sequences in equal yield; G-Y-A6 and G-Y-A7 in the ratio 3:2, and G-N9–16-Y-A2 and G-N9–16-Y-N3 in the ratio 3:2. The significance of these sequences is discussed in relation to the poly(A) content of globin mRNA, the specificity of the sequences, and possible function in processing and biosynthesis of mRNA.

scholarly journals The Structure of High Molecular Weight Ribonucleic Acid in Solution

1961 ◽  
Vol 1 (7) ◽  
pp. 525-537 ◽  
Author(s):  
Serge N. Timasheff ◽  
J. Witz ◽  
V. Luzzati
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid

scholarly journals The presence of a high-molecular-weight (guanine-plus-cytosine)-rich segment at the 3' end of rabbit 28S ribosomal ribonucleic acid.

1975 ◽  
Vol 147 (3) ◽  
pp. 625-628 ◽  
Author(s):  
A A Hadjiolov ◽  
R A Cox ◽  
P Huvos
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Average Molecular Weight ◽  
28S Rrna ◽  
Number Average Molecular Weight ◽  
Ribonuclease T1 ◽  
Naphthoic Acid ◽  
Rrna Molecule ◽  
Ribosomal Ribonucleic Acid

The 3′ hydroxyl end of 28S L-rRNA (major RNA species of the larger subribosomal particle) was labelled by coupling its 2-hydroxy-3-naphthoic acid hydrazine with diazotized [3H]aniline. The RNA was hydrolysed partially with ribonuclease T1 and fractionated on Sephadex G-200. The results show that a highly structured segment with 78% G+C content and a number-average molecular weight of at least 1.0×10(5)-1.8×10(5) is located at the 3′ hydroxyl end of the 28S rRNA molecule.

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