scholarly journals The origin of N-terminal residues in acetic acid-soluble calf-skin collagen

1962 ◽  
Vol 83 (2) ◽  
pp. 245-248 ◽  
Author(s):  
FS STEVEN ◽  
GR TRISTRAM
Keyword(s):  
Acetic Acid ◽  
Skin Collagen ◽  
Calf Skin

scholarly journals STUDIES ON THE FORMATION OF COLLAGEN

1958 ◽  
Vol 108 (2) ◽  
pp. 215-226 ◽  
Author(s):  
Jerome Gross
Keyword(s):  
Acetic Acid ◽  
Ionic Strength ◽  
Guinea Pig ◽  
Salt Solution ◽  
Rapid Development ◽  
Neutral Salt ◽  
Salt Solutions ◽  
Pig Skin ◽  
Skin Collagen ◽  
Calf Skin

Precipitation (or gelation) of collagen from cold neutral salt solution induced by warming was shown to be reversible on subsequent cooling. The degree of reversibility of heat precipitation rapidly diminished with time of incubation at 37°C. For calf skin collagen (acetic acid-extracted) and guinea pig skin collagen (crude NaCl extract) in neutral salt solutions (Γ/2 = 0.45) roughly 90 per cent of newly formed gel redissolved on cooling at 2°C.; less than 20 per cent redissolved on cooling gels previously maintained at 37°C. for 24 hours. At physiologic ionic strength the same preparations exhibited much more rapid development of irreversible precipitation, but the same time dependence was clearly evident. Highly purified collagen from crude saline extracts of guinea pig skin exhibited the same phenomenon although the quantitative aspects were somewhat different.

scholarly journals The Location of Cross-links in γ Chains from Calf Skin Collagen

1968 ◽  
Vol 243 (11) ◽  
pp. 2890-2898
Author(s):  
M P Drake ◽  
P F Davison
Keyword(s):  
Skin Collagen ◽  
Cross Links ◽  
Calf Skin

scholarly journals The Viscometric Behavior of Solubilized Calf Skin Collagen at Low Rates of Shear

1966 ◽  
Vol 241 (8) ◽  
pp. 1784-1789
Author(s):  
Leo D. Kahn ◽  
Lee P. Witnauer
Keyword(s):  
Skin Collagen ◽  
Calf Skin

scholarly journals THERMAL DENATURATION OF SOLUBLE CALF-SKIN COLLAGEN

1965 ◽  
Vol 95 (2) ◽  
pp. 350-353 ◽  
Author(s):  
GR TRISTRAM ◽  
J WORRALL ◽  
DC STEER
Keyword(s):  
Thermal Denaturation ◽  
Skin Collagen ◽  
Calf Skin

scholarly journals Completion of the amino acid sequence of the α1 chain from type I calf skin collagen. Amino acid sequence of α1(I)B8

1983 ◽  
Vol 215 (1) ◽  
pp. 183-189 ◽  
Author(s):  
R W Glanville ◽  
D Breitkreutz ◽  
M Meitinger ◽  
P P Fietzek
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequence ◽  
Type I ◽  
Amino Acid Sequence Analysis ◽  
Complete Amino Acid Sequence ◽  
Skin Collagen ◽  
Arginine Residues ◽  
Calf Skin

The complete amino acid sequence of the 279-residue CNBr peptide CB8 from the alpha 1 chain of type I calf skin collagen is presented. It was determined by sequencing overlapping fragments of CB8 produced by Staphylococcus aureus V8 proteinase, trypsin, Endoproteinase Arg-C and hydroxylamine. Tryptic cleavages were also made specific for lysine by blocking arginine residues with cyclohexane-1,2-dione. This completes the amino acid sequence analysis of the 1054-residues-long alpha (I) chain of calf skin collagen.

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