scholarly journals Two-way separation of amino acids and other ninhydrin-reacting substances by high-voltage electrophoresis followed by paper chromatography

1959 ◽  
Vol 72 (4) ◽  
pp. 691-694 ◽  
Author(s):  
Mary L. Efron
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
High Voltage ◽  
High Voltage Electrophoresis

ISOLATION AND STRUCTURE OF URIDINE NUCLEOTIDE-PEPTIDES FROM AEROBACTER CLOACAE NRC 492

1963 ◽  
Vol 41 (1) ◽  
pp. 1065-1072 ◽  
Author(s):  
R. A. Anwar ◽  
C. Roy ◽  
R. W. Watson
Keyword(s):  
Lactic Acid ◽  
Amino Acid ◽  
Lithium Chloride ◽  
Paper Chromatography ◽  
High Voltage ◽  
Chloride Solutions ◽  
Uridine Nucleotide ◽  
Ethanol Extracts ◽  
Dowex 1 ◽  
High Voltage Electrophoresis

Four closely related uridine nucleotide-peptides isolated from ethanol extracts of penicillin-treated Aerobacter cloacae NRC 492 have been partially characterized. Fractionation of dialyzed extracts on Dowex-1-Cl columns with aqueous lithium chloride solutions, precipitation of the nucleotide-peptides with methanol–acetone, and further separation by paper chromatography and high voltage electrophoresis yielded (a) UDP-GNAc-lact.L-ala.D-glu.meso-dap.D-ala,* (b) UDP-GNAc-lact.L-ala.D-glu.meso-dap, (c) UDP-GNAc-lact.L-ala.D-glu., and (d) UDP-GNAc-lactic acid. The amino acid isomers were identified by micro-enzymic and chromatographic methods.

scholarly journals O-Methyl sugars in lipopolysaccharides of Rhodospirillacea. Identification of 3-O-methyl-d-mannose in Rhodopseudomonas viridis and of 4-O-methyl-d-xylose and 3-O-methyl-6-deoxy-d-talose in Rhodopseudomonas palustris respectively

1973 ◽  
Vol 135 (2) ◽  
pp. 293-297 ◽  
Author(s):  
Jürgen Weckesser ◽  
Hubert Mayer ◽  
Inge Fromme
Keyword(s):  
Mass Spectrometry ◽  
Paper Chromatography ◽  
High Voltage ◽  
Optical Rotation ◽  
Rhodopseudomonas Palustris ◽  
Rhodopseudomonas Viridis ◽  
Gram Negative ◽  
Optical Configuration ◽  
Alditol Acetates ◽  
High Voltage Electrophoresis

1. This paper deals with the identification of three O-methyl sugars in lipopolysaccharides isolated from strains of the Gram-negative photosynthetic family Rhodospirillaceae. In addition to the previously described 3-O-methyl-l-xylose, a second O-methyl sugar was encountered in the lipopolysaccharide of Rhodopseudomonas viridis F, namely 3-O-methyl-d-mannose. The lipopolysaccharides of two strains of Rhodopseudomonas palustris (strain 1e5 and 8/1) contain two O-methylsugars, 4-O-methyl-d-xylose and 3-O-methyl-6-deoxy-d-talose (d-acovenose). 4-O-Methyl-d-xylose, but not 3-O-methyl-6-deoxy-d-talose, could be identified in the lipopolysaccharides of the strains K/1 and 2/2 of the same species. 2. The O-methyl sugars described in this communication were isolated by paper chromatography and identified by g.l.c., paper chromatography, high-voltage electrophoresis and mass spectrometry. Besides the genuine sugars, their alditol acetates and their demethylated (parental) forms were investigated. Optical rotation measurements and, in one case, enzymic reactions were used to establish the optical configuration of the sugars under investigation.

ISOLATION AND STRUCTURE OF URIDINE NUCLEOTIDE-PEPTIDES FROM AEROBACTER CLOACAE NRC 492

1963 ◽  
Vol 41 (4) ◽  
pp. 1065-1072 ◽  
Author(s):  
R. A. Anwar ◽  
C. Roy ◽  
R. W. Watson
Keyword(s):  
Lactic Acid ◽  
Amino Acid ◽  
Lithium Chloride ◽  
Paper Chromatography ◽  
High Voltage ◽  
Chloride Solutions ◽  
Uridine Nucleotide ◽  
Ethanol Extracts ◽  
Dowex 1 ◽  
High Voltage Electrophoresis

Four closely related uridine nucleotide-peptides isolated from ethanol extracts of penicillin-treated Aerobacter cloacae NRC 492 have been partially characterized. Fractionation of dialyzed extracts on Dowex-1-Cl columns with aqueous lithium chloride solutions, precipitation of the nucleotide-peptides with methanol–acetone, and further separation by paper chromatography and high voltage electrophoresis yielded (a) UDP-GNAc-lact.L-ala.D-glu.meso-dap.D-ala,* (b) UDP-GNAc-lact.L-ala.D-glu.meso-dap, (c) UDP-GNAc-lact.L-ala.D-glu., and (d) UDP-GNAc-lactic acid. The amino acid isomers were identified by micro-enzymic and chromatographic methods.

Systematic Screening for Abnormal Urinary Constituents

1968 ◽  
Vol 14 (5) ◽  
pp. 418-428 ◽  
Author(s):  
D S Young
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Voltage ◽  
Systematic Screening ◽  
Sugar Separation ◽  
Layer Chromatography ◽  
High Voltage Electrophoresis

Abstract A scheme is described for the systematic screening for amino acids and sugars in urine. High-voltage electrophoresis is used to separate amino acids, and thin-layer chromatography is used for sugar separation. In each case the separated constituents are stained and scanned in a densitometer, and a profile is constructed for comparison against normal profiles.

High-voltage electrophoresis of choline and its esters

1974 ◽  
Vol 89 (1) ◽  
pp. 96-98 ◽  
Author(s):  
Sheila E. Brooker ◽  
Keith J. Harkiss
Keyword(s):  
High Voltage ◽  
High Voltage Electrophoresis
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