scholarly journals The labelling by [14C]amino acids of cell-sap protein in a cell-free system from guinea-pig liver. The site of origin of labelled protein

1958 ◽  
Vol 70 (2) ◽  
pp. 305-313 ◽  
Author(s):  
J. L. Simkin
Keyword(s):  
Amino Acids ◽  
Guinea Pig ◽  
Pig Liver ◽  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Guinea Pig Liver

Studies on the Incorporation of Amino Acids by a Cell-Free System Obtained from Beef Retina

1972 ◽  
Vol 50 (5) ◽  
pp. 581-587 ◽  
Author(s):  
Y. Matuk
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Incubation Medium ◽  
Optimum Concentration ◽  
Free System ◽  
Cell Free System ◽  
A Cell

The incorporation of 14C-leucine into proteins by a cell-free system from beef retina was studied. It was found that the optimum concentration of ATP depended on the concentration of ribosomes in the incubation medium. Very little incorporation of 14C-leucine was observed in the absence of K+. The optimum concentration of phosphocreatine required for incorporation of radioactive leucine depended on the concentration of Mg2+ in the incubation medium, and the optimum concentration of K+ appears to be independent of the concentrations of Mg2+ and phosphocreatine used.Retinol and retinal had no effect, but ethanol markedly inhibited protein synthesis at concentrations higher than 2%.Puromycin (10−4 M) inhibited incorporation of 14C-leucine by about 80%. The degree of inhibition by cycloheximide depended on the concentration of pH 5 fraction in the incubation medium.

SOME PROPERTIES OF APOFERRITIN ISOLATED FROM GUINEA PIG LIVER

1962 ◽  
Vol 40 (1) ◽  
pp. 983-987 ◽  
Author(s):  
Felix Friedberg
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Guinea Pig ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Guinea Pigs ◽  
Dicarboxylic Acids ◽  
Acetate Buffer ◽  
Pig Liver ◽  
Guinea Pig Liver

Apoferritin isolated from livers of guinea pigs and characterized by a s°w,20 of 17.7 and a pI of 4.8 (in acetate buffer Γ/2 0.1) was hydrolyzed with 5.7 N HCl for 22 and 44 hours and its amino acid composition determined. The protein appears rich in dicarboxylic acids and in leucine. The content of sulphur-containing amino acids is fairly small.

Comparison of O2−-producing activity of guinea-pig eosinophils and neutrophils in a cell-free system

1991 ◽  
Vol 100 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Akimasa Someya ◽  
Isao Nagaoka ◽  
Kazuhisa Iwabuchi ◽  
Tatsuhisa Yamashita
Keyword(s):  
Guinea Pig ◽  
Free System ◽  
Cell Free System ◽  
A Cell
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