scholarly journals The estimation of unsaturated fatty acids by reversed-phase partition chromatography

1955 ◽  
Vol 59 (2) ◽  
pp. 309-316 ◽  
Author(s):  
W. Mary L. Crombie ◽  
R. Comber ◽  
S. G. Boatman

The constitution of the hydrocarbon fraction has been determined by gas/liquid chromatography. Ten alkanes containing both odd and even numbers of carbon atoms have been found. Nonacosane (93 %) and hentriacontane (3 %) are the main components. The constitution of the nonacosane derivatives has been elucidated by subjecting each fraction to modified Baeyer-Villiger oxidation and identifying the key fragments by a combination of adsorption and reversed-phase partition chromatography on films of adsorbent supported on glass plates. A pair of isomers has been found in each group. Those from the ketone and secondary alcohol are derived by substitution of oxygen atoms in the 10- and 15-positions. In the ketol the oxygen substituents are also in the 10- and 15-positions and the isomers are derived by interchanging the positions of the hydroxyl and carbonyl groups.


1961 ◽  
Vol 33 (11) ◽  
pp. 1547-1552 ◽  
Author(s):  
A. G. Hamlin ◽  
B. J. Roberts ◽  
W. Loughlin ◽  
S. G. Walker

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