scholarly journals A new method of large-scale preparation of hypertensin, with a note on its assay

1955 ◽  
Vol 59 (2) ◽  
pp. 300-302 ◽  
Author(s):  
W. S. Peart
Keyword(s):  
Large Scale ◽  
New Method ◽  
Large Scale Preparation ◽  
Scale Preparation

Large-scale preparation of sialyl-Tn antigen-containing peptides from mucin-like glycoproteins in boar seminal gel

2021 ◽  
Author(s):  
Ryota Takeuchi ◽  
Megumi Maeda ◽  
Miran Nakano ◽  
Hiroaki Funahashi ◽  
Yoshinobu Kimura
Keyword(s):  
Tumor Antigen ◽  
Large Scale ◽  
Gel Filtration ◽  
New Method ◽  
Tn Antigen ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Sialyl Tn Antigen ◽  
Unused Resource ◽  
Sialyl Tn

Abstract Sialyl-Tn antigen, a tumor antigen, is a valuable ligand for the purification of proteins that specifically bind to it. Here, we developed a new method for the preparation of large amounts of sialyl-Tn antigen-containing peptides from an unused resource, boar seminal gel. The glycopeptides were prepared from the actinase E digests by a combination of gel filtration and hydrophilic partitioning.

scholarly journals New Method for Large-Scale Preparation of Covalently Closed λ DNA Molecules

1974 ◽  
Vol 14 (5) ◽  
pp. 1104-1107 ◽  
Author(s):  
R. Reuben ◽  
M. Gefter ◽  
L. Enquist ◽  
A. Skalka
Keyword(s):  
Large Scale ◽  
New Method ◽  
Dna Molecules ◽  
Large Scale Preparation ◽  
Scale Preparation

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

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