scholarly journals The application of the ceric sulphate-arsenious acid reaction to the detection of thyroxine and related substances

1955 ◽  
Vol 59 (1) ◽  
pp. 93-97 ◽  
Author(s):  
C. H. Bowden ◽  
N. F. Maclagan ◽  
J. H. Wilkinson
Keyword(s):  
Related Substances ◽  
Arsenious Acid ◽  
Acid Reaction ◽  
Ceric Sulphate

Ceric Sulfate-Arsenious Acid Reaction in Microdetermination of Iodine

1951 ◽  
Vol 23 (10) ◽  
pp. 1507-1510 ◽  
Author(s):  
Allen Lein ◽  
Neena Schwartz
Keyword(s):  
Ceric Sulfate ◽  
Arsenious Acid ◽  
Acid Reaction

A Simple Method for Estimation of Lipoxygenase and Cyclo-Oxygenase Pathways in Human Platelets - the Use of Thiobarbituric Acid Reaction

1980 ◽  
Vol 44 (02) ◽  
pp. 111-114 ◽  
Author(s):  
Hiroshi Takayama ◽  
Minoru Okuma ◽  
Haruto Uchino
Keyword(s):  
Time Course ◽  
Normal Values ◽  
Human Platelet ◽  
Thiobarbituric Acid ◽  
Human Platelets ◽  
Optimal Conditions ◽  
Experimental Conditions ◽  
Simple Method ◽  
Acid Reaction ◽  
Optimal Ph

SummaryTo develop a simple method for estimation of platelet lipoxygenase (PLO) and cyclo-oxygenase (PCO) pathways, the arachidonic acid (AA) metabolism of human platelet was investigated under various experimental conditions by the use of the thiobarbituric acid (TBA) reaction and a radioisotope technique. A TBA-reactive substance different from malondialdehyde (MDA) via PCO pathway was detected and shown to be derived from the PLO pathway. Since the optimal pH and time course of its formation were different from those of MDA formation via PCO pathway, PLO and PCO pathways were estimated by quantitating the TBA-reactive substances produced by the incubation of AA either with aspirin-treated platelets or with untreated ones, respectively, each under optimal conditions. Normal values expressed in terms of nmol MDA/108 platelets were 1.17±0.34 (M±SD, n = 31) and 0.79±0.15 (n = 31) for PLO and PCO pathways, respectively.

Sign in / Sign up

Export Citation Format

Share Document