scholarly journals Relations between metabolism and the rate of turnover of sodium and potassium in guinea pig kidney-cortex slices

1954 ◽  
Vol 56 (3) ◽  
pp. 445-453 ◽  
Author(s):  
R. Whittam ◽  
R. E. Davies
Keyword(s):  
Guinea Pig ◽  
Kidney Cortex ◽  
Pig Kidney ◽  
Cortex Slices ◽  
Kidney Cortex Slices ◽  
Sodium And Potassium

scholarly journals Analyse der strahleninduzierten Elektrolytveränderungen in Nierenschnitten

1960 ◽  
Vol 15 (10) ◽  
pp. 671-675
Author(s):  
H. Breuer ◽  
H. K. Parchwitz
Keyword(s):  
Oxygen Uptake ◽  
Guinea Pig ◽  
Transport Mechanism ◽  
Kidney Cortex ◽  
Concentration Gradients ◽  
Tissue Slices ◽  
Pig Kidney ◽  
X Irradiation ◽  
Cortex Slices ◽  
Kidney Cortex Slices

The effect of X-irradiation (14 000 r-58 000 r) on the movements of potassium and sodium in guinea pig kidney cortex slices after incubation under various conditions has been investigated. In addition, the oxygen uptake of the tissue slices has been measured.The concentration gradients of potassium and sodium between slices and medium were reduced after X-irradiation. However, this reduction was only found when the slices were incubated at 37°C. At lower temperatures no effect of X-irradiation was observed. The oxygen uptake of the kidney cortex slices remained unaffected by X-irradiation.The experiments described in the present paper suggest that X-irradiation acts on the ion transport mechanism itself rather than on energy supplying processes.

scholarly journals Sodium Extrusion and Potassium Uptake in Guinea Pig Kidney Cortex Slices

1965 ◽  
Vol 48 (4) ◽  
pp. 699-717 ◽  
Author(s):  
Guillermo Whittembury
Keyword(s):  
Guinea Pig ◽  
Potential Gradient ◽  
Electrochemical Potential ◽  
Kidney Cortex ◽  
K Uptake ◽  
Pig Kidney ◽  
Cortex Slices ◽  
Sodium Extrusion ◽  
Kidney Cortex Slices ◽  
Bathing Fluid

Slices from the cortex corticis of the guinea pig kidney were immersed in a chilled solution without K and then reimmersed in warmer solutions. The Na and K concentrations and the membrane potential Vm were then studied as a function of the Na and K concentrations of the reimmersion fluid. It was found that Na is extruded from the cells against a large electrochemical potential gradient. Q10 for net Na outflux was ∼2.5. At bath K concentrations larger than 8 mM the behavior of K was largely passive. At the outset of reimmersion (Vm > EK) K influx seemed secondary to Na extrusion. Na extrusion would promote K entrance, being limited and requiring the presence of K in the bathing fluid. At bath K concentrations below 8 mM, K influx was up an electrochemical potential gradient. Thus a parallel active K uptake is apparent. Q10 for net K influx was ∼2.0. Dinitrophenol inhibited net Na outflux and net K influx, Q10 became <1.1 for both fluxes. The ratio between these fluxes varied. Thus at the outset of reimmersion the net Na outflux to net K influx ratio was >1. After 8 minutes it was <1.

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