scholarly journals Studies in detoxication. 38. The metabolism of benzene. (a) The determination of phenylmercapturic acid in urine. (b) Mercapturic acid excretion by rabbits receiving benzene

1951 ◽  
Vol 48 (5) ◽  
pp. 624-629 ◽  
Author(s):  
D. V. Parke ◽  
R. T. Williams
Keyword(s):  
Mercapturic Acid ◽  
Acid Excretion

Simple HPLC Method for Routine Determination of Urinary Phenylacetic Acid Excretion

1993 ◽  
Vol 16 (3) ◽  
pp. 733-741
Author(s):  
E. Griesemann ◽  
S. Bouquet ◽  
M. C. Perault ◽  
S. Favreliere ◽  
L. Barrier ◽  
...  
Keyword(s):  
Phenylacetic Acid ◽  
Hplc Method ◽  
Acid Excretion

Hemoglobin Adducts and Mercapturic Acid Excretion of Acrylamide and Glycidamide in One Study Population

2008 ◽  
Vol 56 (15) ◽  
pp. 6061-6068 ◽  
Author(s):  
Eva C. Hartmann ◽  
Melanie I. Boettcher ◽  
Thomas Schettgen ◽  
Hermann Fromme ◽  
Hans Drexler ◽  
...  
Keyword(s):  
Mercapturic Acid ◽  
Acid Excretion ◽  
Hemoglobin Adducts ◽  
Study Population

Detection of increased bile acid excretion by determination of bile acid content in single stool samples

1984 ◽  
Vol 140 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Horst-Dieter Kaiek ◽  
Frans Stellaard ◽  
Wolfgang Kruis ◽  
Gustav Paumgartner
Keyword(s):  
Bile Acid ◽  
Acid Content ◽  
Acid Excretion ◽  
Bile Acid Excretion ◽  
Stool Samples

scholarly journals An Inter-Laboratory Comparison for the Urinary Acrolein Biomarker 3-Hydroxypropyl-Mercapturic Acid (3-HPMA)

2017 ◽  
Vol 27 (5) ◽  
pp. 65-76
Author(s):  
Gerhard Scherer ◽  
Wolf-Dieter Heller ◽  
Michael McEwan ◽  
Thomas Göen ◽  
Peter Joza ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Coefficient Of Variation ◽  
Solid Phase ◽  
Mercapturic Acid ◽  
Heavy Smoking ◽  
Peak Areas ◽  
Good Repeatability ◽  
Work Up ◽  
Globally Distributed

Summary An inter-laboratory comparison study on the acrolein biomarker of exposure 3-hydroxypropyl-mercapturic acid (3-HPMA) with 12 laboratories from 7 globally distributed countries was performed. The laboratories received coded triplicates of 4 spiked and lyophilized urine samples (LU, 12 samples) as well as 5 authentic urine pool samples (PU, 15 samples) covering the 3-HPMA concentration range from background (non-smoking) to heavy smoking levels for analysis by using their own (in-house) analytical method. All laboratories applied liquid chromatography with tandem mass spectrometry (LC-MS/MS), with most of them (10 of 12) using solid phase extraction (SPE) as sample work-up procedure. The intra-laboratory variation (indicating repeatability) was determined by calculating the standard deviation (sr) and the coefficient of variation (CVr) of the triplicates, whereas the inter-laboratory variation (indicating reproducibility) was determined by calculating the standard deviation between laboratories (sR) and the corresponding coefficient of variation (CVR). After removal of outlier samples or laboratories, the mean CVr values for LU and PU test samples ranged from 2.1–3.6% (mean: 2.8%) and 2.4–3.7% (mean: 3.3%), respectively, indicating good repeatability for the determination of 3-HPMA in both sample types. CVR for LU and PU test samples ranged from 9.1–31.9% (mean: 18.8%) and 13.9–27.0% (mean: 18.5%), respectively, indicating limited reproducibility in 3-HPMA analysis for both sample types. Re-calculation of the PU results by applying an embedded calibration (EC), derived from the reported peak areas for the LU test samples, somewhat improved the CVR values (range: 9.6–28.8%, mean: 16.7%). It is concluded that the intra-laboratory variation (repeatability) in the determination of 3-HPMA in urine is in general acceptable in the participating laboratories, while the inter-laboratory variability requires further improvement. The relatively small reduction in the inter-laboratory variability (sR and CVR) by applying an EC suggests that other methodological factors than the standard reference material for 3-HPMA have to be addressed to achieve further improvement in reproducibility.

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