scholarly journals The stabilization of d-amino-acid oxidase by flavin-adenine dinucleotide, substrates and competitive inhibitors

1951 ◽  
Vol 48 (4) ◽  
pp. 458-467 ◽  
Author(s):  
K. Burton
Keyword(s):  
Amino Acid ◽  
Flavin Adenine Dinucleotide ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Competitive Inhibitors

scholarly journals The Pseudoalteromonas luteoviolacea L-amino Acid Oxidase with Antimicrobial Activity Is a Flavoenzyme

Marine Drugs ◽  
2018 ◽  
Vol 16 (12) ◽  
pp. 499 ◽  
Author(s):  
Andrés Andreo-Vidal ◽  
Antonio Sanchez-Amat ◽  
Jonatan Campillo-Brocal
Keyword(s):  
Antimicrobial Activity ◽  
Amino Acid ◽  
Flavin Adenine Dinucleotide ◽  
Sequence Similarity ◽  
Bacterial Species ◽  
Biological Significance ◽  
Acid Oxidase ◽  
Biotechnological Applications ◽  
Amino Acid Oxidase ◽  
First Time

The marine environment is a rich source of antimicrobial compounds with promising pharmaceutical and biotechnological applications. The Pseudoalteromonas genus harbors one of the highest proportions of bacterial species producing antimicrobial molecules. For decades, the presence of proteins with L-amino acid oxidase (LAAO) and antimicrobial activity in Pseudoalteromonas luteoviolacea has been known. Here, we present for the first time the identification, cloning, characterization and phylogenetic analysis of Pl-LAAO, the enzyme responsible for both LAAO and antimicrobial activity in P. luteoviolacea strain CPMOR-2. Pl-LAAO is a flavoprotein of a broad substrate range, in which the hydrogen peroxide generated in the LAAO reaction is responsible for the antimicrobial activity. So far, no protein with a sequence similarity to Pl-LAAO has been cloned or characterized, with this being the first report on a flavin adenine dinucleotide (FAD)-containing LAAO with antimicrobial activity from a marine microorganism. Our results revealed that 20.4% of the sequenced Pseudoalteromonas strains (specifically, 66.6% of P. luteoviolacea strains) contain Pl-laao similar genes, which constitutes a well-defined phylogenetic group. In summary, this work provides insights into the biological significance of antimicrobial LAAOs in the Pseudoalteromonas genus and shows an effective approach for the detection of novel LAAOs, whose study may be useful for biotechnological applications.

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