scholarly journals On an enzyme from blow-fly larvae [Lucilia sericata] which digests collagen in alkaline solution

1931 ◽  
Vol 25 (5) ◽  
pp. 1458-1463 ◽  
Author(s):  
Ralph Percival Hobson
Keyword(s):  
Alkaline Solution ◽  
Lucilia Sericata ◽  
Blow Fly ◽  
Fly Larvae

scholarly journals Impact of Comingled Heterospecific Assemblages on Developmentally Based Estimates of the Post-Mortem Interval—A Study with Lucilia sericata (Meigen), Phormia regina (Meigen) and Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae)

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 280
Author(s):  
Krystal R. Hans ◽  
Sherah L. Vanlaerhoven
Keyword(s):  
Growth Rate ◽  
Development Time ◽  
Lucilia Sericata ◽  
Phormia Regina ◽  
Calliphora Vicina ◽  
Immature Stages ◽  
Post Mortem ◽  
Blow Fly ◽  
Blow Flies ◽  
Post Mortem Interval

Estimates of the minimum post-mortem interval (mPMI) using the development rate of blow flies (Diptera: Calliphoridae) are common in modern forensic entomology casework. These estimates are based on single species developing in the absence of heterospecific interactions. Yet, in real-world situations, it is not uncommon to have 2 or more blow fly species developing on a body. Species interactions have the potential to change the acceptance of resources as suitable for oviposition, the timing of oviposition, growth rate, size and development time of immature stages, as well as impacting the survival of immature stages to reach adult. This study measured larval development and growth rate of the blow flies Lucilia sericata (Meigen, 1826), Phormia regina (Meigen, 1826) and Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae) over five constant temperatures (15, 20, 25, 30, 35 °C), in the presence of conspecifics or two-species heterospecific assemblages. Temperature and species treatment interacted such that L. sericata larvae gained mass more rapidly when in the presence of P. regina at 20 and 30 °C, however only developed faster at first instar. At later stages, the presence of P. regina slowed development of L. sericata immatures. Development time of C. vicina immatures was not affected by the presence of P. regina, however larvae gained mass more slowly. Development time of P. regina immatures was faster in the presence of either L. sericata or C. vicina until third instar, at which point, the presence of L. sericata was neutral whereas C. vicina negatively impacted development time. Phormia regina larvae gained mass more rapidly in the presence of L. sericata at 20 °C but were negatively impacted at 25 °C by the presence of either L. sericata or C. vicina. The results of this study indicate that metrics such as development time or larval mass used for estimating mPMI with blow flies are impacted by the presence of comingled heterospecific blow fly assemblages. As the effects of heterospecific assemblages are not uniformly positive or negative between stages, temperatures or species combinations, more research into these effects is vital. Until then, caution should be used when estimating mPMI in cases with multiple blow fly species interacting on a body.

Blow Fly Larvae as a Source of Botulinum Toxin for Game Farm Pheasants

1962 ◽  
Vol 26 (4) ◽  
pp. 411 ◽  
Author(s):  
V. H. Lee ◽  
Srikrishnan Vadlamudi ◽  
R. P. Hanson
Keyword(s):  
Botulinum Toxin ◽  
Blow Fly ◽  
Fly Larvae

Colonisation of finfish substrate inhabited by black soldier fly larvae by blow flies, bacteria, and fungi

2020 ◽  
Vol 6 (3) ◽  
pp. 291-304 ◽  
Author(s):  
E. Bernard ◽  
J. Villazana ◽  
A. Alyokhin ◽  
J. Rose
Keyword(s):  
Microbial Communities ◽  
Additional Treatment ◽  
Blow Fly ◽  
Gram Positive ◽  
Black Soldier Fly ◽  
Gram Positive Bacteria ◽  
Fungal Abundance ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
Bacteria And Fungi

Black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) can be used for recycling a wide variety of organic wastes. However, these wastes are also suitable for colonisation by pest fly species, including the blow fly Lucilia sericata (Meigen) (Diptera: Calliphoridae), and by pathogenic microorganisms. We tested effects of the presence of black soldier fly larvae (BSFL) on recruitment and retention of blow fly larvae, as well as on composition and dynamics of microbial communities in finfish-based substrates. Substrates inhabited by BSFL contained approximately one-tenth of the L. sericata pupae found in the control substrate even when BSFL were removed prior to the introduction of gravid L. sericata females. Furthermore, almost three-quarters of L. sericata released into substrates previously colonised by BSFL emigrated within 24 h, while no such emigration was detected for the control substrates. Unlike previous studies, bacterial community was heavily dominated by Gram-positive species. Overall bacterial abundance on BSFL substrates declined approximately two-fold compared to the control substrate, while fungal abundance increased. However, surviving microbial communities were still robust and diverse, and continued to be dominated by Gram-positive bacteria. On the other hand, Gram-negative Shigella sonnei, which is a common cause of diarrhoea in humans, was more than 200 times less abundant in BSFL substrates compared to the control throughout the experiment. Repellent and antibiotic properties observed in this study are advantageous for using BSFL in remediation of finfish wastes. However, additional treatment of remaining residue may still be necessary to eliminate all biological contamination.

Spatial and Seasonal Distribution of Forensically Important Blow Flies (Diptera: Calliphoridae) in Makhanda, Eastern Cape, South Africa

2019 ◽  
Vol 56 (5) ◽  
pp. 1231-1238
Author(s):  
K A Williams ◽  
M H Villet
Keyword(s):  
South Africa ◽  
Seasonal Distribution ◽  
Lucilia Sericata ◽  
Chicken Liver ◽  
Seasonal Activity ◽  
Eastern Cape ◽  
Blow Fly ◽  
Blow Flies ◽  
Management Plans ◽  
Forensic Investigations

AbstractThe seasonal activity of adults of eight forensically important blow fly species [Calliphora croceipalpis (Diptera: Calliphoridae), Jaennicke, Lucilia sericata (Meigen) (Diptera: Calliphoridae), L. cuprina (Wiedemann) (Diptera: Calliphoridae), Chrysomya chloropyga (Wiedemann) (Diptera: Calliphoridae), Ch. albiceps (Wiedemann) (Diptera: Calliphoridae), Ch. marginalis (Wiedemann) (Diptera: Calliphoridae), Ch. putoria (Wiedemann) (Diptera: Calliphoridae), Ch. megacephala (Fabricius) (Diptera: Calliphoridae)] was quantified from collections in Makhanda, South Africa. Flies were collected in traps baited with chicken liver and placed in the field at eight locations for four consecutive days each fortnight for 14 mo. The seasonal distribution of each species compared well to published seasonal distributions of these blow flies elsewhere in South Africa, with evidence of year-to-year variation within seasons that might be explained by weather. This information is important for determining when and where certain species are likely to occur and will be of use in forensic investigations and myiasis management plans.

Characterization of the Volatiles’ Profiles of the Eggs of Forensically Relevant Lucilia sericata and Phormia regina (Diptera: Calliphoridae) Blow Flies by SPME-Facilitated GC-MS

2020 ◽  
Vol 57 (4) ◽  
pp. 994-1005
Author(s):  
Justine E Giffen-Lemieux ◽  
Koji Okuda ◽  
Jennifer Y Rosati ◽  
Rabi A Musah
Keyword(s):  
Solid Phase ◽  
Lucilia Sericata ◽  
Phormia Regina ◽  
Organosulfur Compounds ◽  
Blow Fly ◽  
Headspace Volatiles ◽  
Blow Flies ◽  
Wide Range ◽  
Published Research

Abstract The attraction of necrophagous insects, particularly blow flies, to corpses and carrion is of ecological, economic, and agricultural importance, although the mechanisms by which it occurs are not well understood. Much of the published research on blow fly attractants has focused on volatiles emitted from carrion surrogates, but little attention has been given to the possibility that blow fly eggs themselves may emit chemical cues that are responsible for conspecific and heterospecific insect attraction. In this study, the headspace volatiles emitted from eggs representing two aggregated oviposition events that were collected 1 mo apart from two species of the Calliphoridae family (Order: Diptera), Lucilia sericata (Meigen), and Phormia regina (Meigen) were analyzed via solid-phase microextraction-facilitated GC-MS. The volatiles’ profiles were found to be consistent between samples representing the same species, but unique between the two species. Over 100 molecules covering a wide range of compound classes that included alcohols, aldehydes, esters, amines, ketones, and organosulfur compounds were identified. The profile of volatiles emitted from the L. sericata eggs contained several alkanes and aldehydes, whereas salient features of the P. regina headspace included numerous esters and ketones. Between the two species, 42 compounds were shared, several of which were carboxylic acids. Little overlap between the range of compounds detected and those reported to be emitted from decomposing remains was observed.

A noninclusion virus of blow-fly larvae

1969 ◽  
Vol 13 (2) ◽  
pp. 310-311 ◽  
Author(s):  
W.A.L. David
Keyword(s):  
Blow Fly ◽  
Fly Larvae

Effects of blow fly (Protocalliphora spatulata: Diptera: Calliphoridae) parasitism on the growth of nestling savannah sparrows in Alaska

1997 ◽  
Vol 75 (4) ◽  
pp. 641-644 ◽  
Author(s):  
Clinton K. Miller ◽  
Jeanne M. Fair
Keyword(s):  
Growth Parameters ◽  
Food Limitation ◽  
Blow Fly ◽  
Tarsus Length ◽  
Passerculus Sandwichensis ◽  
Avian Reproduction ◽  
Project Site ◽  
Fly Larvae ◽  
Breeding Seasons ◽  
Savannah Sparrows

We investigated the effect of blow fly larvae (Protocalliphora spatulata) on nestling savannah sparrows (Passerculus sandwichensis) in Alaska subject to biennially fluctuating prey abundance. Grasshopper populations in some regions of interior Alaska can show a strong biennial periodicity. Growth of nestling savannah sparrows, numbers of blow fly larvae in nests and on nestlings, and grasshopper populations were monitored during four breeding seasons (1990–1993) on the Delta Agriculture Project site near Delta Junction, Alaska. Growth parameters for parasitized and nonparasitized nestlings were compared within and between years. There were no significant differences in growth parameters between parasitized and nonparasitized nestlings between any years. There were also no differences in growth parameters within years, except for tarsus length in 1991 and nestling mass in 1993 (low grasshopper years); in 1993, parasitized nestlings were heavier and the sample size was small. Additionally, there were no differences in fledging success within years. Although parasitism combined with other factors (e.g., food limitation) may have an impact on avian reproduction, our data do not support this.

scholarly journals Genome Sequence of aProteus mirabilisStrain Isolated from the Salivary Glands of LarvalLucilia sericata

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Ye Yuan ◽  
Yu Zhang ◽  
Shuhua Fu ◽  
Tawni L. Crippen ◽  
David K. Visi ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Genome Sequence ◽  
Proteus Mirabilis ◽  
Draft Genome ◽  
Lucilia Sericata ◽  
Draft Genome Sequence ◽  
Blow Fly ◽  
Interkingdom Communication

We announce a draft genome sequence of aProteus mirabilisstrain derived fromLucilia sericatasalivary glands. This strain is demonstrated to attract and induce oviposition byL. sericata, a common blow fly important to medicine, agriculture, and forensics. The genome sequence will help dissect interkingdom communication between the species.

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