scholarly journals A beginner’s guide to mass spectrometry–based proteomics

2020 ◽  
Vol 42 (5) ◽  
pp. 64-69
Author(s):  
Ankit Sinha ◽  
Matthias Mann
Keyword(s):  
Analytical Chemistry ◽  
Mass Spectrometry ◽  
Data Analysis ◽  
Sample Preparation ◽  
Single Cell Analysis ◽  
Clinical Applications ◽  
Preparation Process ◽  
Cell Analysis ◽  
Analysis Strategies ◽  
Quantitative Profiling

Mass spectrometry (MS)-based proteomics is the most comprehensive approach for the quantitative profiling of proteins, their interactions and modifications. It is a challenging topic as a firm grasp requires expertise in biochemistry for sample preparation, analytical chemistry for instrumentation and computational biology for data analysis. In this short guide, we highlight the various components of a mass spectrometer, the sample preparation process for conversion of proteins into peptides, and quantification and analysis strategies. The advancing technology of MS-based proteomics now opens up opportunities in clinical applications and single-cell analysis.

scholarly journals High throughput screening of complex biological samples with mass spectrometry – from bulk measurements to single cell analysis

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 872-891 ◽  
Author(s):  
Emily E. Kempa ◽  
Katherine A. Hollywood ◽  
Clive A. Smith ◽  
Perdita E. Barran
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
High Throughput ◽  
High Throughput Screening ◽  
Single Cell Analysis ◽  
State Of The Art ◽  
Cell Analysis ◽  
Biotechnological Applications ◽  
Biological Matrices ◽  
Minimal Sample

We review the state of the art in HTS using mass spectrometry with minimal sample preparation from complex biological matrices. We focus on industrial and biotechnological applications.

Mass Spectrometry for Single-Cell Analysis

2020 ◽  
pp. 1-17
Author(s):  
Dinesh K. Patel ◽  
Sayan Deb Dutta ◽  
Ki-Taek Lim
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

Single-cell analysis by ambient mass spectrometry

2017 ◽  
Vol 90 ◽  
pp. 14-26 ◽  
Author(s):  
Yunyun Yang ◽  
Yanying Huang ◽  
Junhui Wu ◽  
Ning Liu ◽  
Jiewei Deng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Ambient Mass Spectrometry ◽  
Cell Analysis

Single Cell Analysis with Probe ESI-Mass Spectrometry: Detection of Metabolites at Cellular and Subcellular Levels

2014 ◽  
Vol 86 (8) ◽  
pp. 3809-3816 ◽  
Author(s):  
Xiaoyun Gong ◽  
Yaoyao Zhao ◽  
Shaoqing Cai ◽  
Shujie Fu ◽  
Chengdui Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Mass Spectrometry Detection ◽  
Esi Mass Spectrometry

scholarly journals Protocol for Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS: Sample Preparation, Data Acquisition and Analysis

2021 ◽  
Author(s):  
Luis F. Schachner ◽  
Alexander S. Lee ◽  
Neil L. Kelleher
Keyword(s):  
Mass Spectrometry ◽  
Data Analysis ◽  
Mass Spectrum ◽  
Sample Preparation ◽  
Data Acquisition ◽  
Histone Modifications ◽  
Protein Composition ◽  
Ms Analysis ◽  
Level Information ◽  
Single Mass

Abstract The disassembly and digestion of nucleosome particles in current proteomics approaches forfeits correlations among histones and blurs nucleosome-level information. We developed Nuc-MS which analyzes whole nucleosomes and displays their histone modifications and variants in a single mass spectrum. In this protocol, we provide step-by-step instructions for preparation of mononucleosomes for mass spectrometry (MS) analysis, and parameter sets for Nuc-MS data acquisition and data analysis.

scholarly journals Preparation of Tissues and Heterogeneous Cellular Samples for Single-Cell Analysis

2021 ◽  
Author(s):  
E. Celeste Welch ◽  
Anubhav Tripathi
Keyword(s):  
Sample Preparation ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Cell Analysis ◽  
Processing Times ◽  
Tissue Dissociation ◽  
Emerging Trends ◽  
Recent Developments ◽  
Preparation Techniques

While sample preparation techniques for the chemical and biochemical analysis of tissues are fairly well advanced, the preparation of complex, heterogenous samples for single-cell analysis can be difficult and challenging. Nevertheless, there is growing interest in preparing complex cellular samples, particularly tissues, for analysis via single-cell resolution techniques such as single-cell sequencing or flow cytometry. Recent microfluidic tissue dissociation approaches have helped to expedite the preparation of single cells from tissues through the use of optimized, controlled mechanical forces. Cell sorting and selective cellular recovery from heterogenous samples have also gained traction in biosensors, microfluidic systems, and other diagnostic devices. Together, these recent developments in tissue disaggregation and targeted cellular retrieval have contributed to the development of increasingly streamlined sample preparation workflows for single-cell analysis technologies, which minimize equipment requirements, enable lower processing times and costs, and pave the way for high-throughput, automated technologies. In this chapter, we survey recent developments and emerging trends in this field.

Nontargeted Detection Methods for Food Safety and Integrity

2019 ◽  
Vol 10 (1) ◽  
pp. 429-455 ◽  
Author(s):  
Bing Shao ◽  
Hui Li ◽  
Jianzhong Shen ◽  
Yongning Wu
Keyword(s):  
Analytical Chemistry ◽  
Mass Spectrometry ◽  
Food Safety ◽  
Sample Preparation ◽  
Detection Methods ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Chemical Database ◽  
Analytical Strategies ◽  
Food Matrices

Nontargeted workflows for chemical hazard analyses are highly desirable in the food safety and integrity fields to ensure human health. Two different analytical strategies, nontargeted metabolomics and chemical database filtering, can be used to screen unknown contaminants in food matrices. Sufficient mass and chromatographic resolutions are necessary for the detection of compounds and subsequent componentization and interpretation of candidate ions. Analytical chemistry–based technologies, including gas chromatography–mass spectrometry (GC-MS), liquid chromatography–mass spectrometry (LC-MS), nuclear magnetic resonance (NMR), and capillary electrophoresis–mass spectrometry (CE-MS), combined with chemometrics analysis are being used to generate molecular formulas of compounds of interest. The construction of a chemical database plays a crucial role in nontargeted detection. This review provides an overview of the current sample preparation, analytical chemistry–based techniques, and data analysis as well as the limitations and challenges of nontargeted detection methods for analyzing complex food matrices. Improvements in sample preparation and analytical platforms may enhance the relevance of food authenticity, quality, and safety.

Mass Spectrometry for Single-Cell Analysis

2021 ◽  
pp. 1033-1049
Author(s):  
Dinesh K. Patel ◽  
Sayan Deb Dutta ◽  
Ki-Taek Lim
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis
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