scholarly journals Single-molecule detection: Unravelling surface receptor diffusion in live neurons

2005 ◽  
Vol 27 (5) ◽  
pp. 5-8 ◽  
Author(s):  
Laurent Groc ◽  
Daniel Choquet ◽  
Brahim Lounis ◽  
Laurent Cognet
Keyword(s):  
Single Molecule ◽  
Cellular Imaging ◽  
Single Molecule Detection ◽  
Live Cells ◽  
Membrane Diffusion ◽  
Surface Receptor ◽  
Cellular Compartments ◽  
Sub Wavelength ◽  
The Way

Over the last decade, single-molecule detection (SMD) gave biologists a tool to turn their dream, to follow a single molecule in live cells, into reality. SMD provides the advantages of identifying subpopulations and of localizing molecules with sub-wavelength precision. The use of nanometre-sized ligand–fluorophore complexes has even made it possible to track targets within confined cellular compartments. In this review, we first describe the main benefits of SMD in cellular imaging. We then show how SMD was used to unravel the membrane diffusion of glutamatergic receptors and how it sheds light on the way neurons can regulate membrane distribution of receptors.

scholarly journals Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2461 ◽  
Author(s):  
Joshua Welsh ◽  
Julia Kepley ◽  
Ariel Rosner ◽  
Peter Horak ◽  
Jay Berzofsky ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Molecule ◽  
High Refractive Index ◽  
Receptor Expression ◽  
Single Molecule Detection ◽  
Refractive Indices ◽  
Biophysical Properties ◽  
New Class ◽  
Surface Receptor ◽  
Properties Of Materials

Phenotyping extracellular vesicles (EVs), where surface receptor expression is often as low as one molecule per EV, remains problematic due to the inability of commercial flow cytometers to provide single-fluorescent molecule sensitivity. While EVs are widely considered to be of great potential as diagnostic, prognostic and theranostic biomarkers, their use is currently hindered by the lack of tools available to accurately and reproducibly enumerate and phenotype them. Herein, we propose a new class of labels that leverage the biophysical properties of materials with unique complex refractive indices and demonstrate that this class of labels has the possibility of allowing single-epitope detection using conventional flow cytometry.

A Molecular Logic Gate Enables Super-Resolved Imaging of Intracellular Lipid Droplets

2019 ◽  
Author(s):  
Adam Eördögh ◽  
Carolina Paganini ◽  
Dorothea Pinotsi ◽  
Paolo Arosio ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Single Molecule ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Logic Gate ◽  
Living Cells ◽  
Three Dimensions ◽  
Single Molecule Imaging ◽  
Molecular Logic Gate ◽  
Molecular Logic ◽  
Cellular Compartments

<div>Photoactivatable dyes enable single-molecule imaging in biology. Despite progress in the development of new fluorophores and labeling strategies, many cellular compartments remain difficult to image beyond the limit of diffraction in living cells. For example, lipid droplets, which are organelles that contain mostly neutral lipids, have eluded single-molecule imaging. To visualize these challenging subcellular targets, it is necessary to develop new fluorescent molecular devices beyond simple on/off switches. Here, we report a fluorogenic molecular logic gate that can be used to image single molecules associated with lipid droplets with excellent specificity. This probe requires the subsequent action of light, a lipophilic environment and a competent nucleophile to produce a fluorescent product. The combination of these requirements results in a probe that can be used to image the boundary of lipid droplets in three dimensions with resolutions beyond the limit of diffraction. Moreover, this probe enables single-molecule tracking of lipids within and between droplets in living cells.</div>

scholarly journals A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases

2021 ◽  
Author(s):  
Li-juan Wang ◽  
Le Liang ◽  
Bing-jie Liu ◽  
BingHua Jiang ◽  
Chun-yang Zhang
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Amplification Cascade

A controlled T7 transcription-driven symmetric amplification cascade machinery is developed for single-molecule detection of multiple repair glycosylases.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

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