scholarly journals Tissue engineering: Designing for health

2003 ◽  
Vol 25 (5) ◽  
pp. 19-21
Author(s):  
Tim Hardingham
Keyword(s):  
Tissue Engineering ◽  
Biomedical Research ◽  
Gain Control ◽  
Living Cells ◽  
Scientific Understanding ◽  
Biological Processes ◽  
Research Groups ◽  
Living Tissues

The tissue engineering that is now emerging in biomedical research groups is concerned with living tissues and how we can harness biological processes to achieve healing and repair, where it is otherwise failing. It aims to develop our scientific understanding of how living cells function, so that we can gain control and direct their activity to the promote the repair of damaged and diseased tissue1.

Graphene and Graphene-Based Materials in Biomedical Applications

2019 ◽  
Vol 26 (38) ◽  
pp. 6834-6850 ◽  
Author(s):  
Mohammad Omaish Ansari ◽  
Kalamegam Gauthaman ◽  
Abdurahman Essa ◽  
Sidi A. Bencherif ◽  
Adnan Memic
Keyword(s):  
Tissue Engineering ◽  
Thermal Properties ◽  
Gene Delivery ◽  
Regenerative Medicine ◽  
Biomedical Research ◽  
Biomedical Applications ◽  
Biomedical Application ◽  
Two Dimensional ◽  
Drug And Gene Delivery ◽  
Biomedical Fields

: Nanobiotechnology has huge potential in the field of regenerative medicine. One of the main drivers has been the development of novel nanomaterials. One developing class of materials is graphene and its derivatives recognized for their novel properties present on the nanoscale. In particular, graphene and graphene-based nanomaterials have been shown to have excellent electrical, mechanical, optical and thermal properties. Due to these unique properties coupled with the ability to tune their biocompatibility, these nanomaterials have been propelled for various applications. Most recently, these two-dimensional nanomaterials have been widely recognized for their utility in biomedical research. In this review, a brief overview of the strategies to synthesize graphene and its derivatives are discussed. Next, the biocompatibility profile of these nanomaterials as a precursor to their biomedical application is reviewed. Finally, recent applications of graphene-based nanomaterials in various biomedical fields including tissue engineering, drug and gene delivery, biosensing and bioimaging as well as other biorelated studies are highlighted.

Information and scientific impact of Advanced Therapies in the age of mass media: an Altmetrics-based analysis of Tissue Engineering. (Preprint)

2020 ◽  
Author(s):  
Antonio Santisteban ◽  
Julia Moran ◽  
Miguel Ángel Martín Piedra ◽  
Antonio Campos Muñoz ◽  
José Antonio Moral Muñoz ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mass Media ◽  
Prediction Equation ◽  
Factorial Analysis ◽  
Online Media ◽  
Research Groups ◽  
Academic Institutions ◽  
Scientific Impact ◽  
Online Platforms ◽  
The Impact

BACKGROUND Tissue engineering (TE) constitutes a multidisciplinary field aiming to construct artificial tissues to regenerate end-staged organs. Its development has taken placed since the last decade of the 20th century, entailing a clinical revolution. In this sense, TE research groups have worked and shared relevant information in the mass media era. Thus, it would be interesting to study the online dimension of TE research and to compare it with traditional measures of scientific impact. OBJECTIVE To evaluate TE online dimension from 2012 to 2018 by using metadata obtained from the Web of Science (WoS) and Altmetrics and to develop a prediction equation for the impact of TE documents from Almetrics scores. METHODS We have analyzed 23,719 TE documents through descriptive and statistical methods. First, TE temporal evolution was exposed for WoS and fifteen online platforms (News, Blogs, Policy, Twitter, Patents, Peer review, Weibo, Facebook, Wikipedia, Google, Reddit, F1000, Q&A, Video and Mendeley readers). The 10 most-cited TE original articles were ranked according to WoS citations and the Altmetric Attention Score. Second, in order to better comprehend TE online framework, a correlation and factorial analysis were performed based on the suitable results previously obtained for the Bartlett Sphericity and Kaiser-Meyer-Olkin tests. Finally, the liner regression model was applied to elucidate the relation between academy and online media and to construct a prediction equation for TE from Altmetrics data. RESULTS TE dynamic shows an upward trend in WoS citations, Twitter, Mendeley Readers and Altmetric Scores. However, WoS and Altmetrics rankings for the most cited documents clearly differs. When compared, the best correlation results were obtained for Mendeley readers and WoS (ρ=0.71). In addition, the factorial analysis identified six factors that could explain the previously observed differences between TE academy, and the online platforms evaluated. At this point, the mathematical model constructed is able to predict and explain more than the 40% of TE WoS citations from Altmetrics scores. CONCLUSIONS The scientific information related to the construction of bioartificial tissues increasingly reaches society through different online media. Because of the focus of TE research importantly differs when the academic institutions and online platforms are compared, it could be stated that basic and clinical research groups, academic institutions and health politicians should take it into account in a coordinated effort oriented to the design and implementation of adequate strategies for information diffusion and population health education.

Быстродействующая атомно-силовая и сканирующая капиллярная микроскопия в решении задач материаловедения, биологии и медицины

2020 ◽  
Vol 13 (3-4) ◽  
pp. 222-228
Author(s):  
И.В. Яминский ◽  
А.И. Ахметова
Keyword(s):  
Antibiotic Resistance ◽  
Early Detection ◽  
Biomedical Research ◽  
Drug Screening ◽  
Targeted Delivery ◽  
High Speed ◽  
Scanning Probe ◽  
Biological Processes ◽  
Atomic Force ◽  
Probe Microscope

Разработка высокоэффективных режимов быстродействующего сканирующего зондового микроскопа, в первую очередь атомно-силовой и сканирующей капиллярной микроскопии, представляет особый интерес для успешного проведения биомедицинских исследований: изучения биологических процессов и морфологии биополимеров, определения антибио­тикорезистентности бактерий, адресной доставки биомакромолекул, скринингу лекарств, раннему обнаружению биологических агентов (вирусов и бактерий) и др. The development of highly efficient modes of a high-speed scanning probe microscope, primarily atomic force and scanning capillary microscopy, is of particular interest for successful biomedical research: studying biological processes and the morphology of biopolymers, determining antibiotic resistance of bacteria, targeted delivery of biomacromolecules, drug screening, early detection agents (viruses and bacteria), etc.

scholarly journals The emerging technology of biohybrid micro-robots: a review

2021 ◽  
Author(s):  
Zening Lin ◽  
Tao Jiang ◽  
Jianzhong Shang
Keyword(s):  
Self Assembly ◽  
Degrees Of Freedom ◽  
High Energy ◽  
Living Cells ◽  
Multiple Degrees Of Freedom ◽  
The Past ◽  
Great Prevalence ◽  
Performance Decline ◽  
High Energy Efficiency ◽  
Living Tissues

Abstract In the past few decades, robotics research has witnessed an increasingly high interest in miniaturized, intelligent, and integrated robots. The imperative component of a robot is the actuator that determines its performance. Although traditional rigid drives such as motors and gas engines have shown great prevalence in most macroscale circumstances, the reduction of these drives to the millimeter or even lower scale results in a significant increase in manufacturing difficulty accompanied by a remarkable performance decline. Biohybrid robots driven by living cells can be a potential solution to overcome these drawbacks by benefiting from the intrinsic microscale self-assembly of living tissues and high energy efficiency, which, among other unprecedented properties, also feature flexibility, self-repair, and even multiple degrees of freedom. This paper systematically reviews the development of biohybrid robots. First, the development of biological flexible drivers is introduced while emphasizing on their advantages over traditional drivers. Second, up-to-date works regarding biohybrid robots are reviewed in detail from three aspects: biological driving sources, actuator materials, and structures with associated control methodologies. Finally, the potential future applications and major challenges of biohybrid robots are explored. Graphic abstract

scholarly journals Review on Nanocrystalline Cellulose in Bone Tissue Engineering Applications

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2818
Author(s):  
Nur Ilyana Sahira Murizan ◽  
Nur Syahirah Mustafa ◽  
Nor Hasrul Akhmal Ngadiman ◽  
Noordin Mohd Yusof ◽  
Ani Idris
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biomedical Application ◽  
Living Cells ◽  
Nanocrystalline Cellulose ◽  
Isolation Method ◽  
Tissue Engineering Scaffolds ◽  
Comprehensive Knowledge ◽  
Low Toxicity

Nanocrystalline cellulose is an abundant and inexhaustible organic material on Earth. It can be derived from many lignocellulosic plants and also from agricultural residues. They endowed exceptional physicochemical properties, which have promoted their intensive exploration in biomedical application, especially for tissue engineering scaffolds. Nanocrystalline cellulose has been acknowledged due to its low toxicity and low ecotoxicological risks towards living cells. To explore this field, this review provides an overview of nanocrystalline cellulose in designing materials of bone scaffolds. An introduction to nanocrystalline cellulose and its isolation method of acid hydrolysis are discussed following by the application of nanocrystalline cellulose in bone tissue engineering scaffolds. This review also provides comprehensive knowledge and highlights the contribution of nanocrystalline cellulose in terms of mechanical properties, biocompatibility and biodegradability of bone tissue engineering scaffolds. Lastly, the challenges for future scaffold development using nanocrystalline cellulose are also included.

scholarly journals Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3522
Author(s):  
Su Jeong Lee ◽  
Jun Hee Lee ◽  
Jisun Park ◽  
Wan Doo Kim ◽  
Su A Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Research Groups ◽  
Porcine Skin ◽  
Alginate Hydrogel ◽  
Chemical Treatments ◽  
Soft Tissue Engineering

Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.

Raman Imaging of Small Biomolecules

2019 ◽  
Vol 48 (1) ◽  
pp. 347-369 ◽  
Author(s):  
Yihui Shen ◽  
Fanghao Hu ◽  
Wei Min
Keyword(s):  
Imaging Techniques ◽  
Living Cells ◽  
Raman Imaging ◽  
Biological Processes ◽  
Comprehensive Knowledge ◽  
Contrast Mechanisms ◽  
Recent Developments ◽  
Imaging Methodology ◽  
Small Biomolecules ◽  
Microscopic Techniques

Imaging techniques greatly facilitate the comprehensive knowledge of biological systems. Although imaging methodology for biomacromolecules such as protein and nucleic acids has been long established, microscopic techniques and contrast mechanisms are relatively limited for small biomolecules, which are equally important participants in biological processes. Recent developments in Raman imaging, including both microscopy and tailored vibrational tags, have created exciting opportunities for noninvasive imaging of small biomolecules in living cells, tissues, and organisms. Here, we summarize the principle and workflow of small-biomolecule imaging by Raman microscopy. Then, we review recent efforts in imaging, for example, lipids, metabolites, and drugs. The unique advantage of Raman imaging has been manifested in a variety of applications that have provided novel biological insights.

Effects of Process Parameters on Formation of Hybrid Tissue Constructs

2013 ◽  
Author(s):  
Karen Chang Yan ◽  
Pamela Hitscherich ◽  
James Ferrie
Keyword(s):  
Tissue Engineering ◽  
Processing Parameters ◽  
Living Cells ◽  
Live Cells ◽  
Solution Flow Rate ◽  
Electrospinning Technique ◽  
Solution Flow ◽  
Tissue Constructs ◽  
Key Issues ◽  
Working Distance

Tissue engineering is a promising aspect of regenerative medicine that is aimed at constructing functional tissues and organs. While progresses in tissue engineering have led successful clinic applications, challenges remain for more complex tissues/organs that require concerted efforts from multiple types of cells. One of the key issues in building replacements for complex tissues/organs is to mimic the organ’s complex natural organization using a mixture of engineered materials and living cells [1]. Electrospinning has shown promise as a technique to create the microenvironment necessary for cell growth and proliferation for tissue engineering applications[2–4], while multiple fabrication methods have been developed to manipulate live cells(e.g. cell printing) [5–7]. To this end, a system integrating polymer electrospinning technique and pressure-driven cell deposition method is currently under development for forming hybrid tissue constructs with living cells and polymers. This study focuses on examining morphology of electrospun fibers as function of processing parameters including working distance and solution flow rate.

Nanomechanochemical Delivery of Nanoparticles for Nanomechanics Inside Living Cells

2010 ◽  
Author(s):  
Kyungsuk Yum ◽  
Sungsoo Na ◽  
Yang Xiang ◽  
Ning Wang ◽  
Min-Feng Yu
Keyword(s):  
Single Molecule ◽  
Living Cells ◽  
Endocytic Pathway ◽  
Great Promise ◽  
Biological Processes ◽  
Temporal Precision ◽  
Single Molecule Level ◽  
Cellular Processes ◽  
Cellular Environment ◽  
Biological Studies

Studying biological processes and mechanics in living cells is challenging but highly rewarding. Recent advances in experimental techniques have provided numerous ways to investigate cellular processes and mechanics of living cells. However, most of existing techniques for biomechanics are limited to experiments outside or on the membrane of cells, due to the difficulties in physically accessing the interior of living cells. On the other hand, nanomaterials, such as fluorescent quantum dots (QDs) and magnetic nanoparticles, have shown great promise to overcome such limitations due to their small sizes and excellent functionalities, including bright and stable fluorescence and remote manipulability. However, except a few systems, the use of nanoparticles has been limited to the study of biological studies on cell membranes or related to endocytosis, because of the difficulty of delivering dispersed and single nanoparticles into living cells. Various strategies have been explored, but delivered nanoparticles are often trapped in the endocytic pathway or form aggregates in the cytoplasm, limiting their further use. Here we show a nanoscale direct delivery method, named nanomechanochemical delivery, where we manipulate a nanotube-based nanoneedle, carrying “cargo” (QDs in this study), to mechanically penetrate the cell membrane, access specific areas inside cells, and release the cargo [1]. We selectively delivered well-dispersed QDs into either the cytoplasm or the nucleus of living cells. We quantified the dynamics of the delivered QDs by single-molecule tracking and demonstrated the applicability of the QDs as a nanoscale probe for studying nanomechanics inside living cells (by using the biomicrorhology method), revealing the biomechanical heterogeneity of the cellular environment. This method may allow new strategies for studying biological processes and mechanics in living cells with spatial and temporal precision, potentially at the single-molecule level.

A fast-response two-photon fluorescent probe for the detection of Cys over GSH/Hcy with a large turn-on signal and its application in living tissues

2017 ◽  
Vol 5 (1) ◽  
pp. 134-138 ◽  
Author(s):  
Jian-Yong Wang ◽  
Zhan-Rong Liu ◽  
Mingguang Ren ◽  
Xiuqi Kong ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Fast Response ◽  
Living Cells ◽  
Two Photon ◽  
Turn On ◽  
Living Tissues

We developed a novel two-photon fluorescent probe for sensing Cys specifically. The two-photon fluorescent probe exhibited favorable properties and was successfully applied for imaging Cys in living cells and tissues.

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