scholarly journals Genome-wide identification and biochemical characterization of calcineurin B-like calcium sensor proteins in Chlamydomonas reinhardtii

2020 ◽  
Vol 477 (10) ◽  
pp. 1879-1892
Author(s):  
Manoj Kumar ◽  
Komal Sharma ◽  
Akhilesh K. Yadav ◽  
Kajal Kanchan ◽  
Madhu Baghel ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Physiological Processes ◽  
Calcineurin B ◽  
Genome Wide ◽  
Dependent Protein ◽  
Sensor Proteins

Calcium (Ca2+) signaling is involved in the regulation of diverse biological functions through association with several proteins that enable them to respond to abiotic and biotic stresses. Though Ca2+-dependent signaling has been implicated in the regulation of several physiological processes in Chlamydomonas reinhardtii, Ca2+ sensor proteins are not characterized completely. C. reinhardtii has diverged from land plants lineage, but shares many common genes with animals, particularly those encoding proteins of the eukaryotic flagellum (or cilium) along with the basal body. Calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, is an important effector of Ca2+ signaling in animals, while calcineurin B-like proteins (CBLs) play an important role in Ca2+ sensing and signaling in plants. The present study led to the identification of 13 novel CBL-like Ca2+ sensors in C. reinhardtii genome. One of the archetypical genes of the newly identified candidate, CrCBL-like1 was characterized. The ability of CrCBL-like1 protein to sense as well as bind Ca2+ were validated using two-step Ca2+-binding kinetics. The CrCBL-like1 protein localized around the plasma membrane, basal bodies and in flagella, and interacted with voltage-gated Ca2+ channel protein present abundantly in the flagella, indicating its involvement in the regulation of the Ca2+ concentration for flagellar movement. The CrCBL-like1 transcript and protein expression were also found to respond to abiotic stresses, suggesting its involvement in diverse physiological processes. Thus, the present study identifies novel Ca2+ sensors and sheds light on key players involved in Ca2+signaling in C. reinhardtii, which could further be extrapolated to understand the evolution of Ca2+ mediated signaling in other eukaryotes.

scholarly journals Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L.

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247170
Author(s):  
Md. Soyib Hasan ◽  
Vishal Singh ◽  
Shiful Islam ◽  
Md. Sifatul Islam ◽  
Raju Ahsan ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Expression Profiling ◽  
Developmental Stages ◽  
Functional Characterization ◽  
Drought Treatment ◽  
Specific Expression ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Genome Wide

Glutathione transferases (GSTs) constitute an ancient, ubiquitous, multi-functional antioxidant enzyme superfamily that has great importance on cellular detoxification against abiotic and biotic stresses as well as plant development and growth. The present study aimed to a comprehensive genome-wide identification and functional characterization of GST family in one of the economically important legume plants—Medicago truncatula. Here, we have identified a total of ninety-two putative MtGST genes that code for 120 proteins. All these members were classified into twelve classes based on their phylogenetic relationship and the presence of structural conserved domain/motif. Among them, 7 MtGST gene pairs were identified to have segmental duplication. Expression profiling of MtGST transcripts revealed their high level of organ/tissue-specific expression in most of the developmental stages and anatomical tissues. The transcripts of MtGSTU5, MtGSTU8, MtGSTU17, MtGSTU46, and MtGSTU47 showed significant up-regulation in response to various abiotic and biotic stresses. Moreover, transcripts of MtGSTU8, MtGSTU14, MtGSTU28, MtGSTU30, MtGSTU34, MtGSTU46 and MtGSTF8 were found to be highly upregulated in response to drought treatment for 24h and 48h. Among the highly stress-responsive MtGST members, MtGSTU17 showed strong affinity towards its conventional substrates reduced glutathione (GSH) and 1‐chloro‐2,4‐dinitrobenzene (CDNB) with the lowest binding energy of—5.7 kcal/mol and -6.5 kcal/mol, respectively. Furthermore, the substrate-binding site residues of MtGSTU17 were found to be highly conserved. These findings will facilitate the further functional and evolutionary characterization of GST genes in Medicago.

scholarly journals Starch branching enzymes (SBEs) in banana: genome-wide identification and expression analysis reveal their involvement in fruit development, ripening and regulated responses to abiotic/biotic stresses

2018 ◽  
Author(s):  
Hongxia Miao ◽  
Peiguang Sun ◽  
Jiuhua Liu ◽  
Zhiqiang Jin ◽  
Biyu Xu
Keyword(s):  
Fruit Development ◽  
Response Patterns ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Genome Wide ◽  
Fruit Development And Ripening ◽  
Branching Enzymes ◽  
Salt And Drought Stress ◽  
Temporal And Spatial

Starch branching enzyme (SBE), which is one of the key enzymes associated with amylopectin biosynthesis, plays important roles in variable biological processes. Despite its importance, SBE is rarely studied in the banana (Musa acuminata L.) which is a typical starchy fruit. Here, a family of ten SBE proteins (MaSBE) was firstly identified through genome-wide characterization in M. acuminata, which could be clustered into three subfamilies. Systematic transcriptome analysis revealed temporal and spatial expression variations of MaSBE genes and differential response patterns under abiotic and biotic stresses in both banana genotypes, Fen Jiao (FJ) and BaXi Jiao (BX). Moreover, MaSBE2.4 was temporally regulated during fruit development and ripening as well as in response to various abiotic/biotic stresses in both genotypes. Specifically, MaSBE2.3 expression level was higher in FJ than in BX following cold, salt, and drought stress treatments, and it was specifically induced by fungal infection in BX. Characterization of hormone- and stress-related cis-acting elements in the promoters of MaSBE genes suggests their multiple biological functions. In conclusion, our study provides new insights into the complex transcriptional characteristics of the SBE genes, and demonstrates their crucial roles in improving amylopectin biosynthesis and strengthening stress resistance in banana.

scholarly journals Characterization of Germin-like Proteins (GLPs) and Their Expression in Response to Abiotic and Biotic Stresses in Cucumber

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 412
Author(s):  
Liting Liao ◽  
Zhaoyang Hu ◽  
Shiqiang Liu ◽  
Yingui Yang ◽  
Yong Zhou
Keyword(s):  
Stress Response ◽  
Expression Profiles ◽  
Tissue Expression ◽  
Functional Identification ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Genome Wide ◽  
Growth Development ◽  
Tandem Duplications

Germins and germin-like proteins (GLPs) are glycoproteins closely associated with plant development and stress response in the plant kingdom. Here, we carried out genome-wide identification and expression analysis of the GLP gene family in cucumber to study their possible functions. A total of 38 GLP genes were identified in cucumber, which could be mapped to six out of the seven cucumber chromosomes. A phylogenetic analysis of the GLP members from cucumber, Arabidopsis and rice showed that these GLPs could be divided into six groups, and cucumber GLPs in the same group had highly similar conserved motif distribution and gene structure. Gene duplication analysis revealed that six cucumber GLP genes were located in the segmental duplication regions of cucumber chromosomes, while 14 genes were associated with tandem duplications. Tissue expression profiles of cucumber GLP genes showed that many genes were preferentially expressed in specific tissues. In addition, some cucumber GLP genes were differentially expressed under salt, drought and ABA treatments, as well as under DM inoculation. Our results provide important information for the functional identification of GLP genes in the growth, development and stress response of cucumber.

scholarly journals Starch branching enzymes (SBEs) in banana: genome-wide identification and expression analysis reveal their involvement in fruit development, ripening and regulated responses to abiotic/biotic stresses

2018 ◽  
Author(s):  
Hongxia Miao ◽  
Peiguang Sun ◽  
Jiuhua Liu ◽  
Zhiqiang Jin ◽  
Biyu Xu
Keyword(s):  
Fruit Development ◽  
Response Patterns ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Genome Wide ◽  
Fruit Development And Ripening ◽  
Branching Enzymes ◽  
Salt And Drought Stress ◽  
Temporal And Spatial

Starch branching enzyme (SBE), which is one of the key enzymes associated with amylopectin biosynthesis, plays important roles in variable biological processes. Despite its importance, SBE is rarely studied in the banana (Musa acuminata L.) which is a typical starchy fruit. Here, a family of ten SBE proteins (MaSBE) was firstly identified through genome-wide characterization in M. acuminata, which could be clustered into three subfamilies. Systematic transcriptome analysis revealed temporal and spatial expression variations of MaSBE genes and differential response patterns under abiotic and biotic stresses in both banana genotypes, Fen Jiao (FJ) and BaXi Jiao (BX). Moreover, MaSBE2.4 was temporally regulated during fruit development and ripening as well as in response to various abiotic/biotic stresses in both genotypes. Specifically, MaSBE2.3 expression level was higher in FJ than in BX following cold, salt, and drought stress treatments, and it was specifically induced by fungal infection in BX. Characterization of hormone- and stress-related cis-acting elements in the promoters of MaSBE genes suggests their multiple biological functions. In conclusion, our study provides new insights into the complex transcriptional characteristics of the SBE genes, and demonstrates their crucial roles in improving amylopectin biosynthesis and strengthening stress resistance in banana.

scholarly journals Molecular cloning and biochemical characterization of sialidases from zebrafish (Danio rerio)

2007 ◽  
Vol 408 (3) ◽  
pp. 395-406 ◽  
Author(s):  
Marta Manzoni ◽  
Paolo Colombi ◽  
Nadia Papini ◽  
Luana Rubaga ◽  
Natascia Tiso ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Biochemical Characterization ◽  
Chromosome 21 ◽  
Ph Optimum ◽  
Reverse Transcription Pcr ◽  
Genome Wide ◽  
Genome Wide Search ◽  
Putative Orthologues

Sialidases remove sialic acid residues from various sialo-derivatives. To gain further insights into the biological roles of sialidases in vertebrates, we exploited zebrafish (Danio rerio) as an animal model. A zebrafish transcriptome- and genome-wide search using the sequences of the human NEU polypeptides as templates revealed the presence of seven different genes related to human sialidases. neu1 and neu4 are the putative orthologues of the mammalian sialidases NEU1 and NEU4 respectively. Interestingly, the remaining genes are organized in clusters located on chromosome 21 and are all more closely related to mammalian sialidase NEU3. They were thus named neu3.1, neu3.2, neu3.3, neu3.4 and neu3.5. Using RT–PCR (reverse transcription–PCR) we detected transcripts for all genes, apart from neu3.4, and whole-mount in situ hybridization experiments show a localized expression pattern in gut and lens for neu3.1 and neu4 respectively. Transfection experiments in COS7 (monkey kidney) cells demonstrate that Neu3.1, Neu3.2, Neu3.3 and Neu4 zebrafish proteins are sialidase enzymes. Neu3.1, Neu3.3 and Neu4 are membrane-associated and show a very acidic pH optimum below 3.0, whereas Neu3.2 is a soluble sialidase with a pH optimum of 5.6. These results were further confirmed by subcellular localization studies carried out using immunofluorescence. Moreover, expression in COS7 cells of these novel zebrafish sialidases (with the exception of Neu3.2) induces a significant modification of the ganglioside pattern, consistent with the results obtained with membrane-associated mammalian sialidases. Overall, the redundancy of sialidases together with their expression profile and their activity exerted on gangliosides of living cells indicate the biological relevance of this class of enzymes in zebrafish.

scholarly journals In Silico Prediction and Functional Characterization of Genes Related to Abiotic and Biotic Stresses in Chickpea (Cicer arietinum)

2018 ◽  
Vol 7 (1) ◽  
pp. 1-35
Author(s):  
Sukhdeep Kaur ◽  
Satendra Singh ◽  
Gitanjali Tandon ◽  
Sarika Jaiswal ◽  
Mir Asif Iquebal ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
In Silico ◽  
Functional Characterization ◽  
In Silico Prediction ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses

scholarly journals Biochemical Characterization of the Chlamydomonas reinhardtii α-1,4 Glucanotransferase Supports a Direct Function in Amylopectin Biosynthesis

1999 ◽  
Vol 120 (4) ◽  
pp. 1005-1014 ◽  
Author(s):  
Christophe Colleoni ◽  
David Dauvillée ◽  
Gregory Mouille ◽  
Matthew Morell ◽  
Michael Samuel ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biochemical Characterization ◽  
Direct Function
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