scholarly journals CD133-positive dermal papilla-derived Wnt ligands regulate postnatal hair growth

2016 ◽  
Vol 473 (19) ◽  
pp. 3291-3305 ◽  
Author(s):  
Linli Zhou ◽  
Kun Yang ◽  
April Carpenter ◽  
Richard A. Lang ◽  
Thomas Andl ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Proliferation And Differentiation ◽  
Wnt Ligands ◽  
Epithelial Compartment ◽  
Hair Follicle Growth

Active Wnt/β-catenin signaling in the dermal papilla (DP) is required for postnatal hair cycling. In addition, maintenance of the hair-inducing ability of DP cells in vitro requires external addition of Wnt molecules. However, whether DP cells are a critical source of Wnt ligands and induce both autocrine and paracrine signaling cascades to promote adult hair follicle growth and regeneration remains elusive. To address this question, we generated an animal model that allows inducible ablation of Wntless (Wls), a transmembrane Wnt exporter protein, in CD133-positive (CD133+) DP cells. CD133+ cells have been shown to be a specific subpopulation of cells in the DP, which possesses the hair-inducing capability. Here, we show that ablation of Wls expression in CD133+ DP cells results in a shortened period of postnatal hair growth. Mutant hair follicles were unable to enter full anagen (hair growth stage) and progressed toward a rapid regression. Notably, reduced size of the DP and decreased expression of anagen DP marker, versican, were observed in hair follicles when CD133+ DP cells lost Wls expression. Further analysis showed that Wls-deficient CD133+ DP cells led to reduced proliferation and differentiation in matrix keratinocytes and melanocytes that are needed for the generation of the hair follicle structure and a pigmented hair shaft. These findings clearly demonstrate that Wnt ligands produced by CD133+ DP cells play an important role in postnatal hair growth by maintaining the inductivity of DP cells and mediating the signaling cross-talk between the mesenchyme and the epithelial compartment.

scholarly journals Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods

2020 ◽  
Vol 33 (5) ◽  
pp. 280-292
Author(s):  
Ehsan Taghiabadi ◽  
Mohammad Ali Nilforoushzadeh ◽  
Nasser Aghdami
Keyword(s):  
Hair Follicle ◽  
Dermal Papilla ◽  
Culture Conditions ◽  
Hair Follicles ◽  
Main Role ◽  
Follicle Growth ◽  
In Vitro Morphogenesis ◽  
Dermal Papilla Cells ◽  
Hair Follicle Growth

The dermal papilla comprises mesenchymal cells in hair follicles, which play the main role in regulating hair growth. Maintaining the potential hair inductivity of dermal papilla cells (DPCs) and dermal sheath cells during cell culture is the main factor in in vitro morphogenesis and regeneration of hair follicles. Using common methods for the cultivation of human dermal papilla reduces the maintenance requirements of the inductive capacity of the dermal papilla and the expression of specific dermal papilla biomarkers. Optimizing culture conditions is therefore crucial for DPCs. Moreover, exosomes appear to play a key role in regulating the hair follicle growth through a paracrine mechanism and provide a functional method for treating hair loss. The present review investigated the biology of DPCs, the molecular and cell signaling mechanisms contributing to hair follicle growth in humans, the properties of the dermal papilla, and the effective techniques in maintaining hair inductivity in DPC cultures in humans as well as hair follicle bioengineering.

The detection of apoptosis and bcl2 in isolated secondary mohair follicles (growing and quiescent), cultured in vitro in the presence or absence of biotin

1999 ◽  
Vol 1999 ◽  
pp. 159-159
Author(s):  
A.M. Tahmasbi ◽  
H. Galbraith ◽  
J.R. Scaife ◽  
D. Finn
Keyword(s):  
Hair Growth ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Epidermal Cells ◽  
Thymidine Uptake ◽  
Active Process ◽  
Biotin Deficiency ◽  
Proliferation And Differentiation ◽  
Apoptosis Inhibitor

Apoptosis is a physiologically active process whereby cells die in a programmed manner (Stenn et al 1993). Growth of hair occurs as a consequence of proliferation and differentiation of the epidermal cells in the bulb of the hair follicles. Reduction of hair growth or loss of follicle viability may arise from reduction in proliferation, and also from apoptosis of epidermal hair shaft-forming cells. Increases in apoptosis have been associated with reduction in expression of the apoptosis inhibitor bcl2 (Lindner et al 1997). Previous studies (Tahmasbi et al 1998) have shown that biotin deficiency reduced the viability of mohair follicles and thymidine uptake in vitro. The aim of this work was to investigate the presence of apoptosis and bcl2 in follicles which maintained growth or were quiescent following culture in media unsupplemented or supplemented with biotin.

The induction of hair follicle formation in the adult hooded rat by vibrissa dermal papillae

Development ◽  
1970 ◽  
Vol 23 (1) ◽  
pp. 219-236
Author(s):  
R. F. Oliver
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Hair Cycle ◽  
Hair Bulb ◽  
Essential Component ◽  
Dermal Papillae

Hair follicles are essentially composed of two tissues. The inner epidermal component, which gives rise to, among other products, the keratinized hair shaft, is confluent with the surface epidermis and is ensheathed by the dermal component which is confluent with the pars papillaris of the dermis. A specialization of the dermal component is the dermal papilla which, in follicles producing hair, is enclosed by the epidermal matrix of the hair bulb and is connected to the dermal sheath by the papilla stalk. Many authorities have considered that the dermal papilla is an essential component of the hair follicle (reviews: Cohen, 1965; Oliver, 1969). It has been suggested that the dermal papilla may be involved in both the induction of follicle lengthening and hair growth during the proanagen phase (Chase, 1965) of the hair cycle, a concept now justified by direct experimentation in the vibrissa follicle at least (Oliver, 1967b), and perhaps also in determining the nature of the hair produced by a follicle.

Smooth muscle alpha-actin is a marker for hair follicle dermis in vivo and in vitro

1991 ◽  
Vol 99 (3) ◽  
pp. 627-636 ◽  
Author(s):  
C.A. Jahoda ◽  
A.J. Reynolds ◽  
C. Chaponnier ◽  
J.C. Forester ◽  
G. Gabbiani
Keyword(s):  
Smooth Muscle ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Smooth Muscle Alpha Actin ◽  
Actin Expression ◽  
Alpha Actin ◽  
Sheath Cells

We have examined the expression of smooth muscle alpha-actin in hair follicles in situ, and in hair follicle dermal cells in culture by means of immunohistochemistry. Smooth muscle alpha-actin was present in the dermal sheath component of rat vibrissa, rat pelage and human follicles. Dermal papilla cells within all types of follicles did not express the antigen. However, in culture a large percentage of both hair dermal papilla and dermal sheath cells were stained by this antibody. The same cells were negative when tested with an antibody to desmin. Overall, explant-derived skin fibroblasts had relatively low numbers of positively marked cells, but those from skin regions of high hair-follicle density displayed more smooth muscle alpha-actin expression than fibroblasts from areas with fewer follicles. 2-D SDS-PAGE confirmed that, unlike fibroblasts, cultured papilla cells contained significant quantities of the alpha-actin isoform. The rapid switching on of smooth muscle alpha-actin expression by dermal papilla cells in early culture, contrasts with the behaviour of smooth muscle cells in vitro, and has implications for control of expression of the antigen in normal adult systems. The very high percentage of positively marked cultured papilla and sheath cells also provides a novel marker of cells from follicle dermis, and reinforces the idea that they represent a specialized cell population, contributing to the heterogeneity of fibroblast cell types in the skin dermis, and possibly acting as a source of myofibroblasts during wound healing.

scholarly journals 693 Dermal papilla-derived Wnt ligands are required for adult hair follicle growth

2016 ◽  
Vol 136 (5) ◽  
pp. S123
Author(s):  
L. Zhou ◽  
Y. Yang ◽  
T. Andl ◽  
R. Lang ◽  
Y. Zhang
Keyword(s):  
Hair Follicle ◽  
Dermal Papilla ◽  
Follicle Growth ◽  
Wnt Ligands ◽  
Hair Follicle Growth

scholarly journals Dermal exosomes containing miR-218-5p promote hair regeneration by regulating β-catenin signaling

2020 ◽  
Vol 6 (30) ◽  
pp. eaba1685 ◽  
Author(s):  
Shiqi Hu ◽  
Zhenhua Li ◽  
Halle Lutz ◽  
Ke Huang ◽  
Teng Su ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Hair Cycle ◽  
Paracrine Signaling ◽  
Hair Regrowth ◽  
Study Results ◽  
Anagen Phase ◽  
Hair Follicle Cycle

The progression in the hair follicle cycle from the telogen to the anagen phase is the key to regulating hair regrowth. Dermal papilla (DP) cells support hair growth and regulate the hair cycle. However, they gradually lose key inductive properties upon culture. DP cells can partially restore their capacity to promote hair regrowth after being subjected to spheroid culture. In this study, results revealed that DP spheroids are effective at inducing the progression of the hair follicle cycle from telogen to anagen compared with just DP cell or minoxidil treatment. Because of the importance of paracrine signaling in this process, secretome and exosomes were isolated from DP cell culture, and their therapeutic efficacies were investigated. We demonstrated that miR-218-5p was notably up-regulated in DP spheroid–derived exosomes. Western blot and immunofluorescence imaging were used to demonstrate that DP spheroid–derived exosomes up-regulated β-catenin, promoting the development of hair follicles.

scholarly journals Integrin-linked kinase is required for epidermal and hair follicle morphogenesis

2007 ◽  
Vol 177 (3) ◽  
pp. 501-513 ◽  
Author(s):  
Katrin Lorenz ◽  
Carsten Grashoff ◽  
Robert Torka ◽  
Takao Sakai ◽  
Lutz Langbein ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Leading Edge ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Membrane Dynamics ◽  
Epidermal Keratinocytes ◽  
Outer Root Sheath ◽  
Root Sheath ◽  
Integrin Linked Kinase

Integrin-linked kinase (ILK) links integrins to the actin cytoskeleton and is believed to phosphorylate several target proteins. We report that a keratinocyte-restricted deletion of the ILK gene leads to epidermal defects and hair loss. ILK-deficient epidermal keratinocytes exhibited a pronounced integrin-mediated adhesion defect leading to epidermal detachment and blister formation, disruption of the epidermal–dermal basement membrane, and the translocation of proliferating, integrin-expressing keratinocytes to suprabasal epidermal cell layers. The mutant hair follicles were capable of producing hair shaft and inner root sheath cells and contained stem cells and generated proliferating progenitor cells, which were impaired in their downward migration and hence accumulated in the outer root sheath and failed to replenish the hair matrix. In vitro studies with primary ILK-deficient keratinocytes attributed the migration defect to a reduced migration velocity and an impaired stabilization of the leading-edge lamellipodia, which compromised directional and persistent migration. We conclude that ILK plays important roles for epidermis and hair follicle morphogenesis by modulating integrin-mediated adhesion, actin reorganization, and plasma membrane dynamics in keratinocytes.

scholarly journals Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits

2020 ◽  
Vol 40 (2) ◽  
Author(s):  
Zhenyu Wu ◽  
Yanli Zhu ◽  
Hongli Liu ◽  
Gongyan Liu ◽  
Fuchang Li
Keyword(s):  
Cell Cycle ◽  
Signaling Pathway ◽  
Cell Cycle Progression ◽  
Dermal Papilla ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Root Sheath ◽  
Inner Root Sheath

Abstract Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were isolated from Rex rabbits and cultured in vitro to measure hair shaft growth. Meanwhile, dermal papilla cells (DPCs) were isolated and cultured in vitro. Treatment with AdWnt10b or the Wnt/β-Catenin Pathway inhibitor, XAV939, assessed the DPCs proliferation by CCK-8 assay. And the cell cycle was also analyzed by flow cytometry. We found that Wnt10b could promote elongation of the hair shaft, whereas XAV-939 treatment could eliminated this phenomenon. AdWnt10b treatment promoted the proliferation and induced G1/S transition of DPCs. AdWnt10b stimulation up-regulated β-Catenin protein in DPCs. Inhibition of Wnt/β-Catenin signaling by XAV-939 could decreased the basal and Wnt10b-enhanced proliferation of DPCs. And could also suppress the cell cycle progression in DPCs. In summary, our study demonstrates that Wnt10b could promote HFs growth and proliferation of DPCs via the Wnt/β-Catenin signaling pathway in Rex rabbits.

scholarly journals Platelet factor 4 inhibits human hair follicle growth and promotes androgen receptor expression in human dermal papilla cells

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9867
Author(s):  
Ke Sha ◽  
Mengting Chen ◽  
Fangfen Liu ◽  
San Xu ◽  
Ben Wang ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Hair Follicle ◽  
Human Hair ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Follicle Growth ◽  
Human Hair Follicle ◽  
Dermal Papilla Cells ◽  
Platelet Factor ◽  
Hair Follicle Growth

Platelet-rich plasma (PRP) has been reported recently as a potential therapeutic approach for alopecia, such as androgenetic alopecia, but the exact mechanisms and effects of specific components of this recipe remain largely unknown. In this study, we identified that platelet factor 4 (PF4), a component of PRP, significantly suppressed human hair follicle growth and restrained the proliferation of human dermal papilla cells (hDPCs). Furthermore, our results showed that PF4 upregulated androgen receptor (AR) in human dermal papilla cells in vitro and via hair follicle organ culture. Among the hair growth-promoting and DP-signature genes investigated, PF4 decreased the expression of Wnt5a, Wnt10b, LEF1, HEY1 and IGF-1, and increased DKK1 expression, but did not affect BMP2 and BMP4 expression. Collectively, Our data demonstrate that PF4 suppresses human hair follicle growth possibly via upregulating androgen receptor signaling and modulating hair growth-associated genes, which provides thought-provoking insights into the application and optimization of PRP in treating hair loss.

scholarly journals HNG, A Humanin Analogue, Promotes Hair Growth by Inhibiting Anagen-to-Catagen Transition

2020 ◽  
Vol 21 (12) ◽  
pp. 4553
Author(s):  
Sung Min Kim ◽  
Jung-Il Kang ◽  
Hoon-Seok Yoon ◽  
Youn Kyung Choi ◽  
Ji Soo Go ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Cell Cycle Progression ◽  
Dermal Papilla ◽  
Follicle Cell ◽  
Hair Shaft ◽  
Promoting Effect ◽  
Dermal Papilla Cells ◽  
Anagen Phase

The hair follicle goes through repetitive cycles including anagen, catagen, and telogen. The interaction of dermal papilla cells (DPCs) and keratinocytes regulates the hair cycle and hair growth. Humanin was discovered in the surviving brain cells of patients with Alzheimer’s disease. HNG, a humanin analogue, activates cell growth, proliferation, and cell cycle progression, and it protects cells from apoptosis. This study was performed to investigate the promoting effect and action mechanisms of HNG on hair growth. HNG significantly increased DPC proliferation. HNG significantly increased hair shaft elongation in vibrissa hair follicle organ culture. In vivo experiment showed that HNG prolonged anagen duration and inhibited hair follicle cell apoptosis, indicating that HNG inhibited the transition from the anagen to catagen phase mice. Furthermore, HNG activated extracellular signal-regulated kinase (Erk)1/2, Akt, and signal transducer and activator of transcription (Stat3) within minutes and up-regulated vascular endothelial growth factor (VEGF) levels on DPCs. This means that HNG could induce the anagen phase longer by up-regulating VEGF, which is a Stat3 target gene and one of the anagen maintenance factors. HNG stimulated the anagen phase longer with VEGF up-regulation, and it prevented apoptosis by activating Erk1/2, Akt, and Stat3 signaling.

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