scholarly journals p38 mitogen-activated protein kinase (MAPK) first regulates filamentous actin at the 8-16-cell stage during preimplantation development

2005 ◽  
Vol 97 (8) ◽  
pp. 629-640 ◽  
Author(s):  
Andrew J.M. Paliga ◽  
David R. Natale ◽  
Andrew J. Watson
Keyword(s):  
Protein Kinase ◽  
Preimplantation Development ◽  
Mitogen Activated Protein Kinase ◽  
Filamentous Actin ◽  
Cell Stage ◽  
Mitogen Activated Protein

Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse

2000 ◽  
Vol 12 (4) ◽  
pp. 209 ◽  
Author(s):  
Naoki Iwamori ◽  
Kunihiko Naito ◽  
Koji Sugiura ◽  
Hideyuki Kagii ◽  
Masakane Yamashita ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Protein Kinase ◽  
Embryo Development ◽  
Somatic Cells ◽  
Mouse Embryos ◽  
Mapk Cascade ◽  
Mitogen Activated Protein Kinase ◽  
Cell Stage ◽  
Mitogen Activated Protein ◽  
Early Mouse

The mitogen-activated protein kinase (MAPK) cascade is one of the most important signal transduction pathways that regulate the cell cycle in somatic cells. The present study examined the phosphorylation states of components in the MAPK cascade, Raf-1, MEK-1, and extracellular signal regulated kinases (ERKs), which are activated by mitogens, throughout early mouse embryo development and in cultured somatic cells generally. In somatic cells, Raf-1 and MEK-1 were phosphorylated at M-phase and dephosphorylated during interphase. ERKs were not phosphorylated at any stage during the cell cycle. These results were similar to previous findings for the first and second cell cycles of early mouse embryos. In contrast, after the four-cell stage, not only ERKs, but also Raf-1 and MEK-1, were not phosphorylated at any stage during the cell cycle in mouse early embryos. These results suggest that the MAPK cascade in mouse embryos is regulated by the same mechanism as in somatic cells before the two-cell stage, and that regulation is changed to an embryo-specific mechanism after the four-cell stage.

44 Analysis of abnormal chromatin configuration induced by inhibiting MEK at the 1-cell stage

2020 ◽  
Vol 32 (2) ◽  
pp. 147
Author(s):  
K. Magara ◽  
S. Naruto ◽  
R. Watanabe ◽  
T. Wakayama ◽  
S. Kishigami
Keyword(s):  
Protein Kinase ◽  
Histone Modifications ◽  
Blastocyst Stage ◽  
Pericentric Heterochromatin ◽  
Epigenetic Modifications ◽  
Mitogen Activated Protein Kinase ◽  
Cell Stage ◽  
Heterochromatin Formation ◽  
Mitogen Activated Protein ◽  
Kinase Pathway

After fertilisation, mammalian oocytes resume meiosis, leading to pronuclear (PN) formation. It has been known that the mitogen-activated protein kinase pathway negatively regulates PN formation. However, it remains largely unknown how the mitogen-activated protein kinase pathway is involved in the dynamic organisation of chromatin structure in PNs. Here, to address how MEK1/2 activity is involved in PN formation and transcriptional regulation, we examined whether treatment of fertilised oocytes with MEK1/2 inhibitor (PD0325901; PD) during the 1-cell stage affects their global epigenetic modifications and heterochromatin formation in mice. We performed IVF using superovulated oocytes from and ICR strain female mouse and treated IVF embryos with 1 µM PD for 10h from insemination to observe global epigenetic modifications such as H3K9me3 or H3K27me3, localization of heterochromatin protein. First, we found that PD treatment enhanced PN formation with abnormal pronuclear sizes, that is, larger female and smaller male PNs. Further, PD treatment for 10h after fertilisation caused significantly lower global H3K9me3 or H3K27me3 modifications in female PN [H3K9me3: 1.00±0.01 vs. 0.75±0.01 (N>10); P=1.0×10−2, H3K27me3: 1.00±0.01 vs. 0.55±0.01 (N>10); P=6.0×10−7], suggesting failure of establishment of normal histone modifications for heterochromatin. Consistently, we found loss of HP1b around the nucleolus precursor body, which is normally associated with pericentric heterochromatin, suggesting that MEK1/2 activity is required for normal PN and heterochromatin formation. Regardless, embryos treated with PD at the 1-cell stage recovered such global histone modifications through the pre-implantation embryos to reach the blastocyst stage. Together, our data show that MEK1/2 activity plays a pivotal role not only in regulation of PN formation but also heterochromatin formation during the 1-cell stage in mice.

Age-Related Changes in Activation of Mitogen-Activated Protein Kinase Cascades by Oxidative Stress

1998 ◽  
Vol 3 (1) ◽  
pp. 23-27 ◽  
Author(s):  
Kathryn Z Guyton ◽  
Myriani Gorospe ◽  
Xiantao Wang ◽  
Yolanda D Mock ◽  
Gertrude C Kokkonen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Age Related ◽  
Mitogen Activated Protein ◽  
Age Related Changes
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