Improvement in the suspension-culture production of recombinant adeno-associated virus–LacZ in HEK-293 cells using polyethyleneimine–DNA complexes in combination with hypothermic treatment

2008 ◽  
Vol 50 (2) ◽  
pp. 121 ◽  
Author(s):  
Lei Feng ◽  
Meijin Guo ◽  
Shuxiang Zhang ◽  
Ju Chu ◽  
Yingping Zhuang ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Dna Complexes ◽  
Adeno Associated Virus ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Culture Production

scholarly journals Riboswitch-mediated Attenuation of Transgene Cytotoxicity Increases Adeno-associated Virus Vector Yields in HEK-293 Cells

2015 ◽  
Vol 23 (10) ◽  
pp. 1582-1591 ◽  
Author(s):  
Benjamin Strobel ◽  
Benedikt Klauser ◽  
Jörg S Hartig ◽  
Thorsten Lamla ◽  
Florian Gantner ◽  
...  
Keyword(s):  
Virus Vector ◽  
Adeno Associated Virus ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

scholarly journals 465. Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors

2015 ◽  
Vol 23 ◽  
pp. S184-S185
Author(s):  
Daniel R. Blessing ◽  
Monique Montavon ◽  
Maria de Jesus ◽  
Lucia Baldi ◽  
Bernard Schneider ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Adeno Associated Virus ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Shaken Bioreactors

scholarly journals 463. Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors for the Production of Adeno-Associated Virus Vectors

2016 ◽  
Vol 24 ◽  
pp. S183-S184 ◽  
Author(s):  
Daniel R. Blessing ◽  
Bernard Schneider ◽  
Florian M. Wurm ◽  
Nicole Déglon
Keyword(s):  
Adeno Associated Virus ◽  
Hek 293 ◽  
Virus Vectors ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Shaken Bioreactors

Effects of Hydrodynamics on Aggregates Formation, Growth and Metabolism of HEK 293 Cells in Suspension Culture

2006 ◽  
Vol 22 (1) ◽  
pp. 101-106 ◽  
Author(s):  
H LIU ◽  
X LIU ◽  
B WU ◽  
L YE ◽  
X NI ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

scholarly journals Controlling Sheer Stress in a Suspension Culture using Couette Flow for Efficient Proliferation of HEK 293 Cells

2016 ◽  
Vol 03 (01) ◽  
Author(s):  
Katsuhisa Sakaguchi ◽  
Nur Khatijah Mohd Zin ◽  
Yuji Haraguchi ◽  
Azuma Takahashi ◽  
Sara Suzuki ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Couette Flow ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
Sheer Stress ◽  
293 Cells

scholarly journals Characteristics and requirements of basal autophagy in HEK 293 cells

Autophagy ◽  
2013 ◽  
Vol 9 (9) ◽  
pp. 1407-1417 ◽  
Author(s):  
Patience Musiwaro ◽  
Matthew Smith ◽  
Maria Manifava ◽  
Simon A. Walker ◽  
Nicholas T. Ktistakis
Keyword(s):  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

Halothane Inhibition of Recombinant Cardiac L-type Ca2+ Channels Expressed in HEK-293 Cells

2005 ◽  
Vol 103 (6) ◽  
pp. 1156-1166 ◽  
Author(s):  
Kevin J. Gingrich ◽  
Son Tran ◽  
Igor M. Nikonorov ◽  
Thomas J. Blanck
Keyword(s):  
Charge Transfer ◽  
Calcium Channels ◽  
Dependent Manner ◽  
Current Time ◽  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Dependent Inactivation ◽  
Voltage Dependent

Background Volatile anesthetics depress cardiac contractility, which involves inhibition of cardiac L-type calcium channels. To explore the role of voltage-dependent inactivation, the authors analyzed halothane effects on recombinant cardiac L-type calcium channels (alpha1Cbeta2a and alpha1Cbeta2aalpha2/delta1), which differ by the alpha2/delta1 subunit and consequently voltage-dependent inactivation. Methods HEK-293 cells were transiently cotransfected with complementary DNAs encoding alpha1C tagged with green fluorescent protein and beta2a, with and without alpha2/delta1. Halothane effects on macroscopic barium currents were recorded using patch clamp methodology from cells expressing alpha1Cbeta2a and alpha1Cbeta2aalpha2/delta1 as identified by fluorescence microscopy. Results Halothane inhibited peak current (I(peak)) and enhanced apparent inactivation (reported by end pulse current amplitude of 300-ms depolarizations [I300]) in a concentration-dependent manner in both channel types. alpha2/delta1 coexpression shifted relations leftward as reported by the 50% inhibitory concentration of I(peak) and I300/I(peak)for alpha1Cbeta2a (1.8 and 14.5 mm, respectively) and alpha1Cbeta2aalpha2/delta1 (0.74 and 1.36 mm, respectively). Halothane reduced transmembrane charge transfer primarily through I(peak) depression and not by enhancement of macroscopic inactivation for both channels. Conclusions The results indicate that phenotypic features arising from alpha2/delta1 coexpression play a key role in halothane inhibition of cardiac L-type calcium channels. These features included marked effects on I(peak) inhibition, which is the principal determinant of charge transfer reductions. I(peak) depression arises primarily from transitions to nonactivatable states at resting membrane potentials. The findings point to the importance of halothane interactions with states present at resting membrane potential and discount the role of inactivation apparent in current time courses in determining transmembrane charge transfer.

Micropatterned surfaces of PDMS as growth templates for HEK 293 cells

2007 ◽  
Vol 9 (4) ◽  
pp. 475-485 ◽  
Author(s):  
R. M. Johann ◽  
Ch. Baiotto ◽  
Ph. Renaud
Keyword(s):  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells

Calcium Induces Expression of Cytoplasmic Gelsolin in SH-SY5Y and HEK-293 Cells

2010 ◽  
Vol 35 (7) ◽  
pp. 1075-1082 ◽  
Author(s):  
Lina Ji ◽  
Abha Chauhan ◽  
Ved Chauhan
Keyword(s):  
Hek 293 ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Cytoplasmic Gelsolin
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