Non-invasive imaging of firefly luciferase reporter gene expression using bioluminescence imaging in human prostate cancer models

2007 ◽  
Vol 46 (4) ◽  
pp. 179 ◽  
Author(s):  
Jin Zhong Li ◽  
Hongwei Li ◽  
Gregory A. Helm ◽  
Dongfeng Pan
Keyword(s):  
Gene Expression ◽  
Bioluminescence Imaging ◽  
Firefly Luciferase ◽  
Luciferase Reporter ◽  
Human Prostate Cancer ◽  
Luciferase Reporter Gene ◽  
Non Invasive ◽  
Cancer Models ◽  
Firefly Luciferase Reporter ◽  
Firefly Luciferase Reporter Gene

scholarly journals The Coding sequence of firefly luciferase reporter gene affects specific hyperexpressionArabidopsis thaliana cpl1mutant

2017 ◽  
Author(s):  
Hisashi Koiwa ◽  
Akihito Fukudome
Keyword(s):  
Reporter Gene ◽  
Firefly Luciferase ◽  
Luciferase Reporter ◽  
Wild Type ◽  
Coding Region ◽  
Luciferase Reporter Gene ◽  
Coding Sequence ◽  
Firefly Luciferase Reporter ◽  
Endogenous Genes ◽  
Firefly Luciferase Reporter Gene

AbstractForward genetic screening of mutants using firefly luciferase (LUC) reporter gene became a standard practice in plant research. Such screenings frequently identified alleles ofCPL1(Carboxyl-terminal Phosphatase-Like 1) regardless of promoters or pathways studied. Expression of the corresponding endogenous genes often shows the minimal difference between wild type andcpl1.Here we show that theLUCcoding sequence is responsible for the high expression incpl1,using a classicalRD29a-LUC. Deletion of theLUC3’-UTR did not change hyperactivation ofLUCincpl1.However, a codon-modifiedLUC(LUC2) produced similar expression levels both in wild type and incpl1. These results indicate that the coding region ofLUCis responsible for thecpl1-specificLUCoverexpression uncoupled with the expression of the endogenous counterpart.

scholarly journals Transient Transfection of the Zoonotic Parasite Babesia microti

Pathogens ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 108 ◽  
Author(s):  
Mingming Liu ◽  
Shengwei Ji ◽  
Mohamed Abdo Rizk ◽  
Paul Franck Adjou Moumouni ◽  
Eloiza May Galon ◽  
...  
Keyword(s):  
Genetic Manipulation ◽  
Firefly Luciferase ◽  
Transient Transfection ◽  
Babesia Microti ◽  
Luciferase Reporter ◽  
Luciferase Reporter Gene ◽  
Transfection Method ◽  
Firefly Luciferase Reporter ◽  
Zoonotic Parasite ◽  
Firefly Luciferase Reporter Gene

The development of genetic manipulation techniques has been reported in many protozoan parasites over the past few years. However, these techniques have not been established for Babesia microti. Here, we report the first successful transient transfection of B. microti. The plasmids containing the firefly luciferase reporter gene were transfected into B. microti by an AMAXA 4D Nucleofection system. Twenty-four-hour synchronization, the 5′-actin promoter, program FA100, and 50 μg of plasmid DNA constituted the best conditions for the transient transfection of B. microti. This finding is the first step towards a stable transfection method for B. microti, which may contribute to a better understanding of the biology of the parasite.

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