An improved and simplified method for the large-scale purification of pediocin PA-1 produced by Pediococcus acidilactici

2006 ◽  
Vol 43 (2) ◽  
pp. 77 ◽  
Author(s):  
Hafida Aomari ◽  
Lucie Beaulieu ◽  
Denis Groleau ◽  
Muriel Subirade
Keyword(s):  
Large Scale ◽  
Pediococcus Acidilactici ◽  
Simplified Method

scholarly journals STUDY THE GROWTH KINETICS OF PEDIOCOCCUS ACIDILACTICI WITH ESTIMATION OF KINETIC PARAMETERS AND APPLIED IN LARGE SCALE PEDIOCIN PRODUCTION

2016 ◽  
Vol 9 (5) ◽  
pp. 130
Author(s):  
Barnali Mandal
Keyword(s):  
Experimental Data ◽  
Lactic Acid ◽  
Kinetic Parameters ◽  
Growth Kinetics ◽  
Large Scale ◽  
Scale Up ◽  
Small Scale ◽  
Pediococcus Acidilactici ◽  
Monod Model ◽  
Kinetics Of

ABSTRACTObjectives: The aim of the study was to determine the growth kinetics of Pediococcus acidilactici using a mathematical model for large scale pediocinproduction.Methods: Growth kinetics of P. acidilactici has been studied for pediocin production in small scale batch fermenter (Erlenmeyer flask) using meatprocessing waste medium. The experiments have been conducted with varying the concentrations of glucose, protein, and lactic acid. A mathematicalmodel has been developed to describe growth rate, products (pediocin and lactic acid) formation rate, and substrates (glucose and protein) utilizationrate. Monod model for dual substrates (glucose and protein) has been used with considering lactic acid inhibition. Luedeking-Piret model has beenintroduced to describe the production of pediocin and lactic acid.Results: The values of kinetic parameters have been determined using experimental data and model equations. The model prediction has beencompared satisfactorily with the experimental data for the validation of the model.Conclusions: The developed model was satisfactorily validated to scale up the production of pediocin.Keywords: Pediococcus acidilactici, Pediocin, Meat processing waste, Monod model, Luedeking-Piret model, Kinetic parameters.

scholarly journals Growth of Pediococcus acidilactici on sugar cane blackstrap molasses

1998 ◽  
Vol 29 (3) ◽  
pp. 202-207 ◽  
Author(s):  
Ernani S. Sant’Anna ◽  
Regina Coeli O. Torres
Keyword(s):  
Growth Rate ◽  
Carbon Source ◽  
Sugar Cane ◽  
Large Scale ◽  
Pediococcus Acidilactici ◽  
Constant Ph ◽  
Initial Ph ◽  
Main Carbon Source ◽  
Economical Growth ◽  
Main Carbon

Pediococcus acidilactici (IL01) has grown in MRS (Man, Rogosa and Sharpe) broth modified by substitution of glucose by 2.0% (MRS-2), 3.0% (MRS-3), 4.0% (MRS-4) and 5.0% (MRS-5) sugar cane blackstrap molasses. The highest acid production was obtained in MRS-5 broth maintained at a constant pH of 5.0. The highest biomass production was obtained when P. acidilactici was grown in MRS-5 broth at initial pH 6.5, while productivity was higher in MRS-2 broth (28.16%). When the MRS-2 broth was utilized at initial pH 6.5 for a 20-hour fermentation period, the highest growth rate (dx/dt) was found in a period of 8 to 16 hours (0.290 g cells/L.h), while the specific growth rate (µ) was 0.175 (h-1) for that period, differently from the 0.441 (h-1) obtained for the period comprising the 4th to the 12th hour. The growth in MRS broth was 5.08% (2.95 g/l) higher than in MRS-2 broth (2.80 g/l). The data obtained have shown that P. acidilactici has had a significant growth in molasses as the main carbon source, and that it is possible to substitute MRS glucose by this carbon source with the purpose of obtaining a more economical growth medium for the potential large scale productions.

The Performance Analysis and Comparison of the Simplified Method of the Doubly-Fed Wind Generator Model

2013 ◽  
Vol 411-414 ◽  
pp. 1831-1835 ◽  
Author(s):  
Zhi Yan Li ◽  
Qi Hui Liu ◽  
Xiao Ming Wang
Keyword(s):  
Large Scale ◽  
Operating Performance ◽  
High Accuracy ◽  
Doubly Fed Induction Generator ◽  
Simulation Efficiency ◽  
Generator Model ◽  
Simplified Method ◽  
Speed Up ◽  
Doubly Fed ◽  
Control Principle

This article puts forward a simplified method of the doubly-fed induction generator (DFIG for short) model innovatively based on the DFIG control principle. This paper establishes two different degrees of simplification models with equivalence of the converter model and the generator model respectively, and compares the operating performance of each model under the same operating condition. The simulation results show that, the two simplified models have high accuracy, and speed up the simulation efficiency effectively, which is suitable for the study of large-scale wind power generation in power system.

scholarly journals An Experiment-Based Simplified Method for the Model of Building Groups in CFD Simulation

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Hongling Guo ◽  
Ying Zhou ◽  
Yingxin Li ◽  
Zhihui Zhang ◽  
Heng Li
Keyword(s):  
Large Scale ◽  
Cfd Simulation ◽  
Orthogonal Experiment ◽  
Simulation Method ◽  
Edge Length ◽  
Basic Unit ◽  
Simplified Method ◽  
Value Range ◽  
Interval Distance ◽  
Wind Simulation

Computational Fluid Dynamics (CFD) has been widely used in the simulation and analysis of community or urban wind environments. However, the CFD-based wind simulation of large-scale building groups usually consumes a lot of computing resources with high computing costs. To improve the efficiency of CFD-based wind simulation, this paper presents an experiment-based simplified method for the model of building groups. Two rectangular buildings are adopted as the basic unit and four control parameters (B/L, W/L, H/L, and D/L) are selected as the experiment factors to analyze the geometrical relationship of the two buildings. Note that L, W, and H, respectively, represent the windward edge length, width, and height of a building, B is building interval distance, and D is the distance between two building center axes. Then, a single factor experiment and an orthogonal experiment are designed and performed to determine the reasonable value range of each factor. Based on the experiment results and actual situation, the value ranges of four factors for the simplification of building group models are determined as follows: B/L∈{0, 1.5}, W/L∈{0, 2}, D/L∈{0, 0.25}, and H/L∈{0, 1}. Furthermore, a real case is presented to evaluate the performance of the proposed simplified method. The results indicate that the simplified method is able to improve the efficiency of CFD-based wind simulation of building groups, with the number of buildings decreasing from 620 to 395 (by 36.3%), and the number of tetrahedral grids decreasing from 8,832,199 to 7,766,778 (by 12.1%). Thus, this research contributes to the CFD-based wind simulation method of large-scale building groups and the analysis of the urban wind environment.

Production of Bacteriocin by Various Strains of Pediococcus acidilacti during Batch Fermentation and Identification of the Pediocin Structural Genes

2021 ◽  
Vol 37 (4) ◽  
pp. 20-31
Author(s):  
R. Vafadoost ◽  
F.B. Elegado ◽  
D. Aghajani ◽  
M.T.M. Perez
Keyword(s):  
Structural Gene ◽  
Metabolic Pathways ◽  
Batch Fermentation ◽  
Large Scale ◽  
Gene Sequence ◽  
Bacteriocin Production ◽  
Pediococcus Acidilactici ◽  
Structural Genes ◽  
Bacterial Strains ◽  
Grant Support

Bacteriocins are antimicrobial peptides produced by certain bacteria that can be alternatives to traditional antibiotics. This study aimed at evaluating large-scale bacteriocin production by the Pediococcus acidilactici strains in batch fermentation and to analyze the pediocin structural gene (papA) by bioinformatic methods. The fermentation using bacterial strains was carried out in Sartorius Biostat A-Plus Bioreactor, and the bacteriocin production was tested on Listeria innocua as a result of 24 h fermentation. The pediocin structural gene papA was amplified, and the amplicons of each strain were sequenced and analyzed to assess the secondary structure of pediocin and related metabolic pathways. It was shown that the papA structural gene sequence is a conserved region. All strains with a papA amplicon synthesis exhibited active bacteriocin synthesis Keywords: fermentation, Pediococcus acidilactici, purified bacteriocin, pediocin structural gene Funding - The authors acknowledge the grant support by the National Institutes of Molecular Biology and Biotechnology (BIOTECH), Laguna, Philippines.

Purification of Staphylocoagulase by a Bovine Prothrombin-Sepharose 4B Column and its Physicochemical Properties

1979 ◽  
Author(s):  
H. Igarashi ◽  
T. Morita ◽  
S. Iwanaga
Keyword(s):  
Large Scale ◽  
Aureus Strain ◽  
Affinity Column ◽  
Terminal Sequence ◽  
Active Components ◽  
Simplified Method ◽  
Covalently Linked ◽  
Ultracentrifugal Analysis ◽  
Sepharose 4B ◽  
Single Symmetrical Peak

Staphylocoagulase is known to coagulate human plasma, but not bovine’s, by forming an active molecular complex with prothrombin. However, there is no enough knowledges in respect to the molecular mechanism of prothrombin activation with staphylocoasulase. A new simplified method was developed for the large-scale purification of coagulase from culture filtrates of Staphylococcus aureus. strain st-213, using Sepharose AB covalently linked with bovine prothrombin. This affinity column adsorbed strongly the coagulase, which was eluted with 1.0 M NaSCN. The yield of coagulase activity was in the range of 75 to 85X, The purified coagulase showed a single symmetrical peak by ultracentrifugal analysis (S20,w=6.47), and it gave a single precipitin line against anti-purifled staphylocoagulase serum, as revealed by the immunodiffusion test. However, the preparation was shown to contain three active components by the isoelectric foccusing method, suggesting some microheterogeneity. The molecular weight estimated by SDS-gel electrophoresis was 71,000 (major material). No cystine residue was found in the purified material and its NH2-terminal sequence was Ile-Ile-. It is of interest that staphylocoagulase interacts strongly with bovine prothrombin-Sepharose AB, whereas it does not form any active complex with the prothrombin, unlike human prothrombin.

On Simplified Inelastic Method Using Material's Isochronous Stress–Strain Data for Creep Analysis

2013 ◽  
Vol 135 (3) ◽  
Author(s):  
William Koves ◽  
Mingxin Zhao
Keyword(s):  
Elevated Temperature ◽  
Large Scale ◽  
Complex Loading ◽  
Pressure Vessels ◽  
Time Dependent ◽  
Creep Model ◽  
Stress Strain ◽  
Creep Analysis ◽  
Simplified Method ◽  
Strain Method

Design of components or structures at elevated temperature is complex and the use of rigorous time dependent material models may not be practical for many large scale industrial problems. The use of simplified methods permits creep analysis of components that would be impractical by rigorous time dependent models. The isochronous stress–strain method is an approach that has been used extensively for the creep evaluation of elevated temperature components. In practice, the method has been used for the analysis of problems containing both primary and secondary stresses, such as, for pressure vessels with structural discontinuities or creep buckling problems. Although the simplified method has been widely accepted as an alternative to creep analysis, its limitations and accuracy of the method have not been investigated systematically and are not fully understood under complex loading conditions. This study examines the isochronous stress–strain method against a generalized time-explicit creep model. Analytical solutions are developed for three basic loading configurations, including uniaxial tension, pure bending, and torsion, in either load or displacement controlled conditions. Fundamental behaviors of the simplified method are examined and discussed.

A simplified method for large-scale HPA-la phenotyping for antenatal screening

1994 ◽  
Vol 4 (1) ◽  
pp. 21-24 ◽  
Author(s):  
P. Metcalfe ◽  
H. A. Doughty ◽  
M. F. Murphy ◽  
A. H. Waters
Keyword(s):  
Large Scale ◽  
Antenatal Screening ◽  
Simplified Method
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