Purification and conformational properties of a human interferon α2b produced in Escherichia coli

2001 ◽  
Vol 33 (3) ◽  
pp. 173 ◽  
Author(s):  
Alejandro Beldarraín ◽  
Yai Cruz ◽  
Oscar Cruz ◽  
Monica Navarro ◽  
Miriela Gil
Keyword(s):  
Escherichia Coli ◽  
Human Interferon ◽  
Conformational Properties ◽  
Interferon Α2b

The role of lac operon and lac repressor in the induction using lactose for the expression of periplasmic human interferon-α2b by Escherichia coli

2011 ◽  
Vol 62 (4) ◽  
pp. 1427-1435 ◽  
Author(s):  
Joo Shun Tan ◽  
Ramakrishnan Nagasundara Ramanan ◽  
Tau Chuan Ling ◽  
Shuhaimi Mustafa ◽  
Arbakariya B. Ariff
Keyword(s):  
Escherichia Coli ◽  
Lac Repressor ◽  
Human Interferon ◽  
Lac Operon ◽  
Interferon Α2b

scholarly journals Enhanced Interferon-α2b Production in Periplasmic Space of Escherichia coli through Medium Optimization using Response Surface Method

2009 ◽  
Vol 3 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Joo Shun Tan ◽  
Ramakrishnan Nagasundara Ramanan ◽  
Siti Nor Ani Azaman ◽  
Tau Chuan Ling ◽  
Mustapha Shuhaimi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Response Surface ◽  
Yeast Extract ◽  
Nitrogen Sources ◽  
Luria Bertani ◽  
Human Interferon ◽  
Shake Flask Culture ◽  
Periplasmic Space ◽  
Medium Components ◽  
Interferon Α2b

The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone.. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively.

Preparative method for obtaining recombinant human interferon α2b from inclusion bodies of Escherichia coli

2016 ◽  
Vol 42 (6) ◽  
pp. 631-637 ◽  
Author(s):  
V. P. Romanov ◽  
T. I. Kostromina ◽  
A. I. Miroshnikov ◽  
S. A. Feofanov
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Preparative Method ◽  
Human Interferon ◽  
Interferon Α2b

scholarly journals Proteome analysis of Escherichia coli periplasmic proteins in response to over-expression of recombinant human interferon α2b

2014 ◽  
Vol 36 (7) ◽  
pp. 1479-1484 ◽  
Author(s):  
Somayyeh Heidary ◽  
Raha Abdul Rahim ◽  
Samira Eissazadeh ◽  
Hassan Moeini ◽  
Adam Leow Thean Chor ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Proteome Analysis ◽  
Human Interferon ◽  
Over Expression ◽  
Periplasmic Proteins ◽  
Interferon Α2b

Optimization of the primary recovery of human interferon α2b from Escherichia coli inclusion bodies

2006 ◽  
Vol 45 (1) ◽  
pp. 226-234 ◽  
Author(s):  
C.A. Valente ◽  
G.A. Monteiro ◽  
J.M.S. Cabral ◽  
M. Fevereiro ◽  
D.M.F. Prazeres
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Human Interferon ◽  
Interferon Α2b

scholarly journals Optimization of osmotic shock process variables for enhancement of the release of periplasmic interferon-α2b from Escherichia coli using response surface method

2010 ◽  
Vol 45 (2) ◽  
pp. 196-202 ◽  
Author(s):  
Ramakrishnan Nagasundara Ramanan ◽  
Joo Shun Tan ◽  
Mohd Shamzi Mohamed ◽  
Tau Chuan Ling ◽  
Beng Ti Tey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Response Surface ◽  
Response Surface Method ◽  
Osmotic Shock ◽  
Process Variables ◽  
Shock Process ◽  
Surface Method ◽  
Interferon Α2b
Sign in / Sign up

Export Citation Format

Share Document