Enzyme catalysis and transition structures in vacuo. Transition structures for the enolization, carboxylation and oxygenation reactions in ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme (Rubisco)

1994 ◽  
Vol 90 (16) ◽  
pp. 2365-2374 ◽  
Author(s):  
O. Tapia ◽  
J. Andrés ◽  
V. S. Safont
Keyword(s):  
Enzyme Catalysis ◽  
Bisphosphate Carboxylase ◽  
Transition Structures

Enzyme catalysis: Transition structures and quantum dynamical aspects: Modeling rubisco's oxygenation and carboxylation mechanisms

2002 ◽  
Vol 88 (1) ◽  
pp. 154-166 ◽  
Author(s):  
O. Tapia ◽  
Henk Fidder ◽  
Vicent S. Safont ◽  
Mónica Oliva ◽  
Juan Andrés
Keyword(s):  
Enzyme Catalysis ◽  
Quantum Dynamical ◽  
Transition Structures

Transition structures for hydride transfer reactions in vacuo and their role in enzyme catalysis

1996 ◽  
Vol 371 ◽  
pp. 299-312 ◽  
Author(s):  
J. Andrés ◽  
V. Moliner ◽  
V.S. Safont ◽  
J.M. Aulló ◽  
W. Díaz ◽  
...  
Keyword(s):  
Enzyme Catalysis ◽  
Hydride Transfer ◽  
Transfer Reactions ◽  
Transition Structures ◽  
Hydride Transfer Reactions

Three-dimensional distribution of ribulose-1, 5-bisphosphate carboxylase/oxygemase during the early development of proplastids in dark-grown Euglena cells transferred to an inorganic medium

1990 ◽  
Vol 48 (3) ◽  
pp. 906-907
Author(s):  
Tomoko Ehara ◽  
Shuji Sumida ◽  
Tetsuaki Osafune ◽  
Eiji Hase
Keyword(s):  
Cell Suspension ◽  
Euglena Gracilis ◽  
Carbon Materials ◽  
Three Dimensional ◽  
Peripheral Region ◽  
Plastid Development ◽  
Bisphosphate Carboxylase ◽  
Inorganic Medium ◽  
Ribulose 1 ◽  
Dimensional Distribution

As shown previously, Euglena cells grown in Hutner’s medium in the dark without agitation accumulate wax as well as paramylum, and contain proplastids showing no internal structure except for a single prothylakoid existing close to the envelope. When the cells are transferred to an inorganic medium containing ammonium salt and the cell suspension is aerated in the dark, the wax was oxidatively metabolized, providing carbon materials and energy 23 for some dark processes of plastid development. Under these conditions, pyrenoid-like structures (called “pro-pyrenoids”) are formed at the sites adjacent to the prolamel larbodies (PLB) localized in the peripheral region of the proplastid. The single prothylakoid becomes paired with a newly formed prothylakoid, and a part of the paired prothylakoids is extended, with foldings, in to the “propyrenoid”. In this study, we observed a concentration of RuBisCO in the “propyrenoid” of Euglena gracilis strain Z using immunoelectron microscopy.

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