What base pairings can occur in DNA? A distributed multipole study of the electrostatic interactions between normal and alkylated nucleic acid bases

1993 ◽  
Vol 89 (18) ◽  
pp. 3407 ◽  
Author(s):  
Sarah L. Price ◽  
Fabrizio Lo Celso ◽  
Julia A. Treichel ◽  
Julia M. Goodfellow ◽  
Yagnesh Umrania
Keyword(s):  
Nucleic Acid ◽  
Electrostatic Interactions ◽  
Nucleic Acid Bases

scholarly journals Self-assembling ferritin-dendrimer nanoparticles for targeted delivery of nucleic acids to myeloid leukemia cells

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Federica Palombarini ◽  
Silvia Masciarelli ◽  
Alessio Incocciati ◽  
Francesca Liccardo ◽  
Elisa Di Fabio ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Targeted Delivery ◽  
Myeloid Leukemia ◽  
Electrostatic Interactions ◽  
Archaeoglobus Fulgidus ◽  
Leukemia Cells ◽  
Cellular Delivery ◽  
Self Assembling ◽  
Wide Range ◽  
Hybrid Nanoparticle

Abstract Background In recent years, the use of ferritins as nano-vehicles for drug delivery is taking center stage. Compared to other similar nanocarriers, Archaeoglobus fulgidus ferritin is particularly interesting due to its unique ability to assemble-disassemble under very mild conditions. Recently this ferritin was engineered to get a chimeric protein targeted to human CD71 receptor, typically overexpressed in cancer cells. Results Archaeoglobus fulgidus chimeric ferritin was used to generate a self-assembling hybrid nanoparticle hosting an aminic dendrimer together with a small nucleic acid. The positively charged dendrimer can indeed establish electrostatic interactions with the chimeric ferritin internal surface, allowing the formation of a protein-dendrimer binary system. The 4 large triangular openings on the ferritin shell represent a gate for negatively charged small RNAs, which access the internal cavity attracted by the dense positive charge of the dendrimer. This ternary protein-dendrimer-RNA system is efficiently uptaken by acute myeloid leukemia cells, typically difficult to transfect. As a proof of concept, we used a microRNA whose cellular delivery and induced phenotypic effects can be easily detected. In this article we have demonstrated that this hybrid nanoparticle successfully delivers a pre-miRNA to leukemia cells. Once delivered, the nucleic acid is released into the cytosol and processed to mature miRNA, thus eliciting phenotypic effects and morphological changes similar to the initial stages of granulocyte differentiation. Conclusion The results here presented pave the way for the design of a new family of protein-based transfecting agents that can specifically target a wide range of diseased cells. Graphic abstract

Thermochemistry of aqueous solutions of alkylated nucleic acid bases

1979 ◽  
Vol 10 (3-4) ◽  
pp. 415-421 ◽  
Author(s):  
Anna Zielenkiewicz ◽  
Ewa Plesiewicz ◽  
Kazimierz L. Wierzchowski
Keyword(s):  
Nucleic Acid ◽  
Aqueous Solutions ◽  
Nucleic Acid Bases

Tautomerism of nucleic acid bases. I. Cytosine

1972 ◽  
Vol 94 (3) ◽  
pp. 951-959 ◽  
Author(s):  
Gregory C. Y. Lee ◽  
J. H. Prestegard ◽  
Sunney I. Chan
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Bases
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