Characterisation of aerosol OT-stabilised oil-in-water microemulsions using a time-resolved fluorescence method

1987 ◽  
Vol 83 (5) ◽  
pp. 1493 ◽  
Author(s):  
Paul D. I. Fletcher
Keyword(s):  
Fluorescence Method ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Aerosol Ot ◽  
Oil In Water

A sensitive sandwich structure time-resolved fluorescence method for thrombin detection

2018 ◽  
Vol 10 (34) ◽  
pp. 4178-4182 ◽  
Author(s):  
Tao Wu ◽  
Yuanfu Zhang ◽  
Tingting Hou ◽  
Yinghong Zhang ◽  
Shuhao Wang
Keyword(s):  
Sandwich Structure ◽  
Fluorescence Assay ◽  
Fluorescence Method ◽  
Specific Time ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Human Thrombin

We designed a sensitive and specific time-resolved fluorescence assay for detection of human thrombin.

STATIC and TIME-RESOLVED FLUORESCENCE OF AN AMPHIPHILIC FLAVIN IN AEROSOL OT REVERSED MICELLES

1987 ◽  
Vol 46 (4) ◽  
pp. 457-466 ◽  
Author(s):  
Antonie J. W. G. Visser ◽  
Kees Vos ◽  
Jillert S. Santema ◽  
Jan Bouwstra ◽  
Arie VAN Hoek
Keyword(s):  
Reversed Micelles ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Aerosol Ot

Ultrasensitive Time-Resolved Fluorescence Method for α-Fetoprotein

1990 ◽  
Vol 36 (8) ◽  
pp. 1497-1502 ◽  
Author(s):  
Theodore K Christopoulos ◽  
Evrikiia S Lianidou ◽  
Eleftherios P Diamandis
Keyword(s):  
Dicarboxylic Acid ◽  
Fluorescence Method ◽  
Maximum Sensitivity ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Normal Individuals ◽  
Sandwich Type ◽  
Sample Dilution

Abstract We have examined the maximum sensitivity of a newly developed and optimized time-resolved fluorescence immunoassay system. The system, originally described elsewhere Clin Biochem 1988;21:139-50), has undergone significant improvements in sensitivity through improvements of the labeled reagent used. We have chosen an α-fetoprotein [Illegible Text] assay as a model and used monoclonal "capture" antibodies and monoclonal or polyclonal biotinylated antibodies in "sandwich-type" assay configurations. Streptavidin labeled with the europium chelator 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid was used for detection. We can measure as few as 3 x 105 molecules of [Illegible Text] with the optimized system. We have applied this assay to measure AFP in the serum of normal individuals after a 10-fold sample dilution. We conclude that this system is extremely sensitive and can be used in immunoassay or other applications where biotinylated reagents can be applied.

scholarly journals The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreea Lorena Mateescu ◽  
Nicolae-Bogdan Mincu ◽  
Silvana Vasilca ◽  
Roxana Apetrei ◽  
Diana Stan ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Protein Complexes ◽  
C Reactive Protein ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Reactive Protein ◽  
In Vitro Diagnostic ◽  
The Stability ◽  
Positive Controls

AbstractThe purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C.

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