Induction of mesenchymal stem cell differentiation by co-culturing with mature cells in double-layered 2-methacryloyloxyethyl phosphorylcholine polymer hydrogel matrices

2021 ◽  
Author(s):  
Kazuhiko Ishihara ◽  
Miu Kaneyasu ◽  
Kyoko Fukazawa ◽  
Ren Zhang ◽  
Yuji Teramura
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Water Soluble ◽  
Polymer Hydrogel ◽  
Differentiated Cells ◽  
Mesenchymal Stem Cell Differentiation ◽  
Hydrogel Matrix ◽  
Zwitterionic Polymer ◽  
A Cell

The effects of differentiated cells on stem cell differentiation were analyzed by co-culture using a cell-encapsulated double-layered hydrogel system. As a polymer hydrogel matrix, a water-soluble zwitterionic polymer having both...

A cancellous bone matrix system with specific mineralisation degrees for mesenchymal stem cell differentiation and bone regeneration

2019 ◽  
Vol 7 (6) ◽  
pp. 2452-2467 ◽  
Author(s):  
Shijie Liu ◽  
Yiyun Wang ◽  
Jian Wang ◽  
Pengcheng Qiu ◽  
Shengyu Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Bone Matrix ◽  
Stem Cell Differentiation ◽  
Matrix System ◽  
Mesenchymal Stem Cell Differentiation ◽  
Regenerative Therapies

Bone regenerative therapies have been explored using various biomaterial systems.

scholarly journals Nuclear Export of Smads by RanBP3L Regulates Bone Morphogenetic Protein Signaling and Mesenchymal Stem Cell Differentiation

2015 ◽  
Vol 35 (10) ◽  
pp. 1700-1711 ◽  
Author(s):  
Fenfang Chen ◽  
Xia Lin ◽  
Pinglong Xu ◽  
Zhengmao Zhang ◽  
Yanzhen Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Nuclear Export ◽  
Stem Cell Differentiation ◽  
Bmp Signaling ◽  
Dependent Manner ◽  
Mesenchymal Stem Cell Differentiation

Bone morphogenetic proteins (BMPs) play vital roles in regulating stem cell maintenance and differentiation. BMPs can induce osteogenesis and inhibit myogenesis of mesenchymal stem cells. Canonical BMP signaling is stringently controlled through reversible phosphorylation and nucleocytoplasmic shuttling of Smad1, Smad5, and Smad8 (Smad1/5/8). However, how the nuclear export of Smad1/5/8 is regulated remains unclear. Here we report that the Ran-binding protein RanBP3L acts as a nuclear export factor for Smad1/5/8. RanBP3L directly recognizes dephosphorylated Smad1/5/8 and mediates their nuclear export in a Ran-dependent manner. Increased expression of RanBP3L blocks BMP-induced osteogenesis of mouse bone marrow-derived mesenchymal stem cells and promotes myogenic induction of C2C12 mouse myoblasts, whereas depletion of RanBP3L expression enhances BMP-dependent stem cell differentiation activity and transcriptional responses. In conclusion, our results demonstrate that RanBP3L, as a nuclear exporter for BMP-specific Smads, plays a critical role in terminating BMP signaling and regulating mesenchymal stem cell differentiation.

scholarly journals SHP1 Regulates Bone Mass by Directing Mesenchymal Stem Cell Differentiation

Cell Reports ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. 769-780 ◽  
Author(s):  
Menghui Jiang ◽  
Chunxing Zheng ◽  
Peishun Shou ◽  
Na Li ◽  
Gang Cao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Bone Mass ◽  
Stem Cell Differentiation ◽  
Mesenchymal Stem Cell Differentiation
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