Injectable silk nanofiber hydrogels as stem cell carriers to accelerate wound healing

2021 ◽  
Vol 9 (37) ◽  
pp. 7771-7781
Author(s):  
Jiadai Li ◽  
Zhaozhao Ding ◽  
Xin Zheng ◽  
Guozhong Lu ◽  
Qiang Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Hair Follicle ◽  
Cell Behaviors ◽  
Accelerate Wound Healing ◽  
Cell Carriers

Injectable silk nanofiber hdyrogels were used to load stem cells and regulate cell behaviors effectively. The stem cell–laden silk hydrogels accelerated wound healing with higher quality and hair follicle regeneration.

scholarly journals Extracellular caspase-1 regulates hair follicle stem cell migration during wound-healing

2019 ◽  
Author(s):  
Subhasri Ghosh ◽  
Akhil SHP Ananthan ◽  
Sunny Kataria ◽  
Neha Pincha ◽  
Abhik Dutta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Hair Follicle ◽  
Healing Process ◽  
Environmental Cues ◽  
Macromolecular Complex ◽  
Caspase 1 ◽  
Hair Follicle Stem Cells ◽  
Follicle Stem Cells

AbstractMigration of stem cells from one niche to another is a fundamental behavior observed during tissue morphogenesis, homeostasis, and repair 1. A common thread running throughout these phenomena is the ability of stem cells to sense their environmental cues that, in turn, regulate their spatiotemporal localization with amazing precision. Perturbations of such cellular responses underlie a spectrum of pathologies ranging from developmental defects, tumor metastasis and ineffective wound closure 2,3. In somatic tissues, the wound-healing process is a paradigm of the directed migration of various stem cell pools to the site of injury where they differentiate to replenish lost or damaged cells. While there has been substantial investment and progress in understanding the lineage trajectory of stem cells once they reach their destination, comparatively little is understood regarding the mechanisms guiding their chemotactic journey to the wound site. In the context of the skin, it has been shown nearly two decades ago that wounding activates various local epithelial stem cell pools, including multipotent hair follicle stem cells, to infiltrate the epidermis where they participate in the reconstruction of the damaged tissue 4,5. However, elucidation of the environmental cues that coax these cells out of their hair follicle niche to the damaged epidermis has proven to be an intractable problem to solve. Using both an excisional wound and genetic mouse models of wound healing, we discovered that wounded keratinocytes secrete the enzyme Caspase-1. This protein is classically known as a critical component of the cytosolic macromolecular complex called an inflammasome that mediates the unconventional secretion of various cytokines including IL-1a 6. Surprisingly, we find that the released caspase-1 itself has a non-canonical role in the extracellular milieu. Through the Caspase Activation Recruitment Domain (CARD) of caspase-1, this protein is sufficient to initiate chemotaxis of hair follicle stem cells into the epidermis. The secretion of caspase-1 has also been documented in many other pathological scenarios7,8 and we observed that the migration of HFSCs into the epidermis following UV irradiation of the skin is also caspase-1 dependent. Uncovering this novel function of Caspase-1 facilitates a deeper understanding of the mechanistic basis of the epithelial hyperplasia found to accompany numerous inflammatory skin diseases.

scholarly journals Hair-follicle mesenchymal stem-cell activity during homeostasis and wound healing

2021 ◽  
Author(s):  
Emil Aamar ◽  
Efrat Avigad Laron ◽  
Wisal Asaad ◽  
Sarina Harshuk-Shabso ◽  
David Enshell-Seijffers
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Cell Activity ◽  
Stem Cell Activity

scholarly journals A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths

2020 ◽  
Vol 15 (1) ◽  
pp. 41-50
Author(s):  
Jingxu Guo ◽  
Shuwei Li ◽  
Hongyang Wang ◽  
Tinghui Wu ◽  
Zhenhui Wu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mouse Model ◽  
Hair Follicle ◽  
Smooth Muscle Actin ◽  
Hair Follicles ◽  
Alpha Smooth Muscle Actin ◽  
Papillary Structure ◽  
Glass Membrane

AbstractObjectiveStem cells hold promise for treating hair loss. Here an in vitro mouse model was developed using outer root sheaths (ORSs) isolated from hair follicles for studying stem cell-mediated dermal papillary regeneration.MethodsUnder sterile conditions, structurally intact ORSs were isolated from hair follicles of 3-day-old Kunming mice and incubated in growth medium. Samples were collected daily for 5 days. Stem cell distribution, proliferation, differentiation, and migration were monitored during regeneration.ResultsCell proliferation began at the glass membrane periphery then spread gradually toward the membrane center, with the presence of CD34 and CD200 positive stem cells involved in repair initiation. Next, CD34 positive stem cells migrated down the glass membrane, where some participated in ORS formation, while other CD34 cells and CD200 positive cells migrated to hair follicle centers. Within the hair follicle matrix, stem cells divided, grew, differentiated and caused outward expansion of the glass membrane to form a dermal papillary structure containing alpha-smooth muscle actin. Neutrophils attracted to the wound site phagocytosed bacterial and cell debris to protect regenerating tissue from infection.ConclusionIsolated hair follicle ORSs can regenerate new dermal papillary structures in vitro. Stem cells and neutrophils play important roles in the regeneration process.

scholarly journals Therapeutic Potential of Stem Cells in Follicle Regeneration

2018 ◽  
Vol 2018 ◽  
pp. 1-16 ◽  
Author(s):  
Agnieszka Owczarczyk-Saczonek ◽  
Magdalena Krajewska-Włodarczyk ◽  
Anna Kruszewska ◽  
Łukasz Banasiak ◽  
Waldemar Placek ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Hair Follicle ◽  
Therapeutic Potential ◽  
Cell Activity ◽  
Hair Follicles ◽  
Treatment Technique ◽  
Androgenic Alopecia ◽  
The Metabolic Syndrome ◽  
Increased Risk

Alopecia is caused by a variety of factors which affect the hair cycle and decrease stem cell activity and hair follicle regeneration capability. This process causes lower self-acceptance, which may result in depression and anxiety. However, an early onset of androgenic alopecia is associated with an increased incidence of the metabolic syndrome and an increased risk of the cardiac ischaemic disease. The ubiquity of alopecia provides an encouragement to seek new, more effective therapies aimed at hair follicle regeneration and neoregeneration. We know that stem cells can be used to regenerate hair in several therapeutic strategies: reversing the pathological mechanisms which contribute to hair loss, regeneration of complete hair follicles from their parts, and neogenesis of hair follicles from a stem cell culture with isolated cells or tissue engineering. Hair transplant has become a conventional treatment technique in androgenic alopecia (micrografts). Although an autologous transplant is regarded as the gold standard, its usability is limited, because of both a limited amount of material and a reduced viability of cells obtained in this way. The new therapeutic options are adipose-derived stem cells and stem cells from Wharton’s jelly. They seem an ideal cell population for use in regenerative medicine because of the absence of immunogenic properties and their ease of obtainment, multipotential character, ease of differentiating into various cell lines, and considerable potential for angiogenesis. In this article, we presented advantages and limitations of using these types of cells in alopecia treatment.

scholarly journals The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healing

2018 ◽  
Vol 10 (432) ◽  
pp. eaai8524 ◽  
Author(s):  
Xiaoxing Kou ◽  
Xingtian Xu ◽  
Chider Chen ◽  
Maria Laura Sanmillan ◽  
Tao Cai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Accelerate Wound Healing

scholarly journals Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Dajeong Yoon ◽  
Dogeon Yoon ◽  
Heejoong Sim ◽  
Inseok Hwang ◽  
Ji-Seon Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Pressure Ulcer ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Type I ◽  
Skin Ulcers

Fibroblasts synthesize and secrete dermal collagen, matrix proteins, growth factors, and cytokines. These characteristics of fibroblasts provide a potential way for fibroblast therapy to treat skin ulcers more effectively than conventional therapies such as cytokine therapy and negative pressure wound therapy. However, the obstacle to the commercialization of fibroblast therapy is the limited supply of cells with consistent quality. In this study, we tested whether human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) could be differentiated into fibroblasts considering that they have characteristics of high differentiation rates, unlimited proliferation possibility from a single colony, and homogeneity. As a result, hESC-MSC-derived fibroblasts (hESC-MSC-Fbs) showed a significant increase in the expression of type I and III collagen, fibronectin, and fibroblast-specific protein-1 (FSP-1). Besides, vessel formation and wound healing were enhanced in hESC-MSC-Fb-treated skin tissues compared to PBS- or hESC-MSC-treated skin tissues, along with decreased IL-6 expression at 4 days after the formation of pressure ulcer wound in a mouse model. In view of the limited available cell sources for fibroblast therapy, hESC-MSC-Fbs show a promising potential as a commercial cell therapy source to treat skin ulcers.

scholarly journals Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth-Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 466 ◽  
Author(s):  
Pietro Gentile ◽  
Simone Garcovich
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Growth ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Hair Loss ◽  
Hair Growth ◽  
Follicle Development ◽  
Hair Regrowth ◽  
Hair Follicle Development

The use of stem cells has been reported to improve hair regrowth in several therapeutic strategies, including reversing the pathological mechanisms, that contribute to hair loss, regeneration of hair follicles, or creating hair using the tissue-engineering approach. Although various promising stem cell approaches are progressing via pre-clinical models to clinical trials, intraoperative stem cell treatments with a one-step procedure offer a quicker result by incorporating an autologous cell source without manipulation, which may be injected by surgeons through a well-established clinical practice. Many authors have concentrated on adipose-derived stromal vascular cells due to their ability to separate into numerous cell genealogies, platelet-rich plasma for its ability to enhance cell multiplication and neo-angiogenesis, as well as human follicle mesenchymal stem cells. In this paper, the significant improvements in intraoperative stem cell approaches, from in vivo models to clinical investigations, are reviewed. The potential regenerative instruments and functions of various cell populaces in the hair regrowth process are discussed. The addition of Wnt signaling in dermal papilla cells is considered a key factor in stimulating hair growth. Mesenchymal stem cell-derived signaling and growth factors obtained by platelets influence hair growth through cellular proliferation to prolong the anagen phase (FGF-7), induce cell growth (ERK activation), stimulate hair follicle development (β-catenin), and suppress apoptotic cues (Bcl-2 release and Akt activation).

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