A coumarin-based TICT fluorescent probe for real-time fluorescence lifetime imaging of mitochondrial viscosity and systemic inflammation in vivo

2021 ◽  
Vol 9 (38) ◽  
pp. 8067-8073
Author(s):  
Yun Liang ◽  
Yuping Zhao ◽  
Chaofeng Lai ◽  
Xiang Zou ◽  
Weiying Lin
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Systemic Inflammation ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Viscosity Change ◽  
Lifetime Imaging ◽  
Nir Fluorescence ◽  
In Cells

A novel NIR fluorescence lifetime probe Mito-VCI specifically tracked mitochondrial viscosity change in cells and successfully achieved systemic inflammation detection in vivo via FLIM.

scholarly journals In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera

2021 ◽  
Author(s):  
Jason T. Smith ◽  
Alena Rudkouskaya ◽  
Shan Gao ◽  
Juhi M. Gupta ◽  
Arin Ulku ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Near Infrared ◽  
Single Photon ◽  
In Vivo Studies ◽  
Fluorescence Lifetime Imaging ◽  
Iccd Camera ◽  
Lifetime Imaging ◽  
Nir Fluorescence

Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche photodiode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM) applications, but their suitability for in vivo macroscopic FLI (MFLI) in deep tissues remains to be demonstrated. Herein, we report in vivo NIR MFLI measurement with SwissSPAD2, a large time-gated SPAD camera. We first benchmark its performance in well-controlled in vitro experiments, ranging from monitoring environmental effects on fluorescence lifetime, to quantifying Förster Resonant Energy Transfer (FRET) between dyes. Next, we use it for in vivo studies of target-drug engagement in live and intact tumor xenografts using FRET. Information obtained with SwissSPAD2 was successfully compared to that obtained with a gated-ICCD camera, using two different approaches. Our results demonstrate that SPAD cameras offer a powerful technology for in vivo preclinical applications in the NIR window.

scholarly journals In Vivo Fluorescence Lifetime Imaging for Monitoring the Efficacy of the Cancer Treatment

2014 ◽  
Vol 20 (13) ◽  
pp. 3531-3539 ◽  
Author(s):  
Yasaman Ardeshirpour ◽  
Victor Chernomordik ◽  
Moinuddin Hassan ◽  
Rafal Zielinski ◽  
Jacek Capala ◽  
...  
Keyword(s):  
Cancer Treatment ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Fluorescence

scholarly journals Fluorescent probe for visualizing guanine-quadruplex DNA by fluorescence lifetime imaging microscopy

2013 ◽  
Vol 18 (10) ◽  
pp. 101309 ◽  
Author(s):  
Ting-Yuan Tseng ◽  
Cheng-Hao Chien ◽  
Jen-Fei Chu ◽  
Wei-Chun Huang ◽  
Mei-Ying Lin ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Quadruplex Dna ◽  
Lifetime Imaging ◽  
Guanine Quadruplex

scholarly journals Coarse-grained modeling of mitochondrial metabolism enables subcellular flux inference from fluorescence lifetime imaging microscopy

2020 ◽  
Author(s):  
Xingbo Yang ◽  
Daniel J. Needleman
Keyword(s):  
Fluorescence Lifetime ◽  
Metabolic Flux ◽  
Living Cells ◽  
Coarse Grained ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Metabolic Fluxes ◽  
Subcellular Resolution

AbstractMitochondria are central to metabolism and their dysfunctions are associated with many diseases1–9. Metabolic flux, the rate of turnover of molecules through a metabolic pathway, is one of the most important quantities in metabolism, but it remains a challenge to measure spatiotemporal variations in mitochondrial metabolic fluxes in living cells. Fluorescence lifetime imaging microscopy (FLIM) of NADH is a label-free technique that is widely used to characterize the metabolic state of mitochondria in vivo10–18. However, the utility of this technique has been limited by the inability to relate FLIM measurement to the underlying metabolic activities in mitochondria. Here we show that, if properly interpreted, FLIM of NADH can be used to quantitatively measure the flux through a major mitochondrial metabolic pathway, the electron transport chain (ETC), in vivo with subcellular resolution. This result is based on the use of a coarse-grained NADH redox model, which we test in mouse oocytes subject to a wide variety of perturbations by comparing predicted fluxes to direct biochemical measurements and by self-consistency criterion. Using this method, we discovered a subcellular spatial gradient of mitochondrial metabolic flux in mouse oocytes. We showed that this subcellular variation in mitochondrial flux correlates with a corresponding subcellular variation in mitochondrial membrane potential. The developed model, and the resulting procedure for analyzing FLIM of NADH, are valid under nearly all circumstances of biological interest. Thus, this approach is a general procedure to measure metabolic fluxes dynamically in living cells, with subcellular resolution.

Towards in-vivo assessment of fluorescence lifetime: imaging using time-gated intensified CCD camera

2018 ◽  
Vol 38 (4) ◽  
pp. 966-974 ◽  
Author(s):  
Piotr Sawosz ◽  
Stanislaw Wojtkiewicz ◽  
Michal Kacprzak ◽  
Elzbieta Zieminska ◽  
Magdalena Morawiec ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Ccd Camera ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
In Vivo Assessment
Sign in / Sign up

Export Citation Format

Share Document