Thermosensitive polymer hydrogel as a physical shield on colonic mucosa for colitis treatment

2021 ◽  
Author(s):  
Zhaoyuan Guo ◽  
Yun Bai ◽  
Zhuangzhuang Zhang ◽  
Heng Mei ◽  
Jing Li ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Colonic Mucosa ◽  
Potential Treatment ◽  
Thermosensitive Polymer ◽  
Polymer Hydrogel

A thermo-sensitive polymer hydrogel as a physical shield and drug depot was developed for the potential treatment of ulcerative colitis.

Serpin B1 protects colonic epithelial cell via blockage of neutrophil elastase activity and its expression is enhanced in patients with ulcerative colitis

2012 ◽  
Vol 302 (10) ◽  
pp. G1163-G1170 ◽  
Author(s):  
Kazuhiko Uchiyama ◽  
Yuji Naito ◽  
Tomohisa Takagi ◽  
Katsura Mizushima ◽  
Yasuko Hirai ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Mrna Expression ◽  
Real Time ◽  
Neutrophil Elastase ◽  
Colonic Mucosa ◽  
Clinical Samples ◽  
Active Ulcerative Colitis ◽  
Inflammatory Infiltration ◽  
Serpin B1

Serpin B1 is a monocyte neutrophil elastase (NE) inhibitor and is one of the most efficient inhibitors of NE. In the present study, we investigated the role of serpin B1 in the pathogenesis of ulcerative colitis by using clinical samples and an experimental model. The colonic expression of serpin B1 was determined by real-time polymerase chain reaction (PCR), Western blot analysis, and immunohistological studies in both normal and inflamed mucosa from patients with ulcerative colitis. Serpin B1 mRNA expression was determined by real-time PCR in the mouse dextran sodium sulfate (DSS)-induced colitis model. Young adult mouse colonic epithelial (YAMC) cells were used to determine the role of serpin B1. Serpin B1 gene transfected YAMC cells were treated with H2O2 to measure cell viability. The expression of NE was determined in YAMC cells treated with H2O2. NE-silenced YAMC cells were also treated with H2O2 and then measured for viability. Upregulated expression of serpin B1 in colonic mucosa was confirmed from patients with active ulcerative colitis. Immunohistochemical studies showed that serpin B1 expression was localized not only in inflammatory infiltration cells but also in epithelial cells. Serpin B1 mRNA expression was also increased in colonic mucosa of mouse DSS-induced colitis. Serpin B1-transfected YAMC cells were resistant against the treatment of H2O2. H2O2 treatment significantly induced NE in YAMC cells, and NE-silenced YAMC cells were also resistant against the treatment of H2O2. These results suggest that serpin B1 may be a novel marker of active ulcerative colitis and may play an important role in the pathogenesis of inflammatory bowel disease.

Fusobacterium varium localized in the colonic mucosa of patients with ulcerative colitis stimulates species-specific antibody

2002 ◽  
Vol 17 (8) ◽  
pp. 849-853 ◽  
Author(s):  
Toshifumi Ohkusa ◽  
Nobuhiro Sato ◽  
Tatuo Ogihara ◽  
Koji Morita ◽  
Masayuki Ogawa ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Specific Antibody ◽  
Colonic Mucosa ◽  
Species Specific ◽  
Fusobacterium Varium

scholarly journals Psyllium and fat in diets differentially affect the activities and expressions of colonic sphingomyelinases and caspase in mice

2004 ◽  
Vol 91 (5) ◽  
pp. 715-723 ◽  
Author(s):  
Yajun Cheng ◽  
Lena Ohlsson ◽  
Rui-Dong Duan
Keyword(s):  
Ulcerative Colitis ◽  
Alkaline Phosphatase ◽  
High Fat Diet ◽  
Colonic Mucosa ◽  
Caspase 3 ◽  
High Fat ◽  
Acid And Alkaline Phosphatase ◽  
Alkaline Sphingomyelinase ◽  
Opposite Manner ◽  
Colonic Tumours

Dietary fibre and fat affect colonic tumourigenesis and inflammation. Sphingomyelin metabolism may have implications for the pathogenesis of colonic tumours and ulcerative colitis. The present study examined the effects of psyllium and fat on the enzymes responsible for sphingomyelin metabolism and apoptosis in the colon. Mice were fed control, psyllium-containing (100 g/kg), high-fat (313 g/kg, 53 % energy as fat) or high-fat plus psyllium diets for 4 weeks. The activities of acid, neutral and alkaline sphingomyelinase (SMase), neutral ceramidase, and caspase 3, 8 and 9 in colonic mucosa were determined. The expressions of alkaline SMase and caspase 3 were examined. The psyllium-containing diet was found to increase significantly the activities of alkaline SMase and caspase 3 and decreased those of acid SMase and neutral ceramidase. The high-fat diet had opposite effects on these enzymes and attenuated the effects of psyllium. Western blotting showed that psyllium increased and high-fat decreased the levels of alkaline SMase and caspase 3 in colonic mucosa. The change in caspase 3 activity was positively correlated with that of alkaline SMase and negatively with acid SMase. No similar changes of acid and alkaline phosphatase activities in the colon or acid and neutral SMase activity in the liver were identified. In conclusion, colonic sphingomyelin metabolism and apoptosis were affected by psyllium and fat in an opposite manner. The results may have implications for colorectal tumourigenesis and inflammation.

Tu1700 Increased Abundance of Bacteria Producing Hydrogen Sulfide in the Colonic Mucosa of Patients With Ulcerative Colitis and Crohn's Disease

2013 ◽  
Vol 144 (5) ◽  
pp. S-825
Author(s):  
Franck Carbonero ◽  
Ann C. Benefiel ◽  
Jona Kristo ◽  
Jenna K. Leinberger ◽  
Maeve M. Leurck ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Hydrogen Sulfide ◽  
Crohn's Disease ◽  
Colonic Mucosa

scholarly journals Decreased expression of thioredoxin interacting protein mRNA in inflamed colonic mucosa in patients with ulcerative colitis

2007 ◽  
Author(s):  
Yasuo Takahashi ◽  
Hideki Masuda ◽  
Yukimoto Ishii ◽  
Yayoi Nishida ◽  
Megumi Kobayashi ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Colonic Mucosa ◽  
Interacting Protein ◽  
Thioredoxin Interacting Protein

scholarly journals Hsa_circ_0001021 regulates intestinal epithelial barrier function via sponging miR-224-5p in ulcerative colitis

Epigenomics ◽  
2021 ◽  
Author(s):  
Bing Li ◽  
Yan Li ◽  
Lixiang Li ◽  
Yu Yu ◽  
Xiang Gu ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Functional Analysis ◽  
Barrier Function ◽  
Colonic Mucosa ◽  
Clinical Samples ◽  
Epithelial Barrier ◽  
Healthy Controls ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Epithelial Barrier Function

Aims: Few circRNAs have been thoroughly explored in ulcerative colitis (UC). Materials & methods: Microarrays and qualitative real-time PCR were used to detect and confirm dysregulated circRNAs associated with UC. Functional analysis was performed to explore the roles. Results: A total of 580 circRNAs and 87 miRNAs were simultaneously dysregulated in both inflamed and noninflamed UC colonic mucosa compared with healthy controls. Accordingly, hsa_circ_0001021 was significantly downregulated in patients with UC and was related to Mayo scores. Clinical samples and cell experiments revealed that hsa_circ_0001021 was expressed in epithelial cells and correlated with ZO-1, occludin and CLDN-2. Moreover, hsa_circ_0001021 sponged miR-224-5p to upregulate smad4 and increased ZO-1 and occludin. Conclusion: Hsa_circ_0001021 is related to UC severity and regulates epithelial barrier function via sponging miR-224-5p.

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