The effects of electric fields on the mechanical properties and microstructure of ex-vivo porcine brain tissues

Soft Matter ◽  
2021 ◽  
Author(s):  
Chi Zhang ◽  
Hongwei Zhao
Keyword(s):  
Mechanical Properties ◽  
Electric Fields ◽  
Ex Vivo ◽  
Clinical Applications ◽  
Brain Diseases ◽  
Physiological Conditions ◽  
Porcine Brain ◽  
The Brain ◽  
Brain Tissues

As a popular tool for regulating the physiological conditions of the brain and treating brain diseases, electrotherapy has become increasingly mature in clinical applications. However, the mechanical properties and microstructure...

scholarly journals Norepinephrine Transporter Imaging in the Brain of a Rat Model of Depression Using Radioiodinated (2S, αS)-2-(α-(2-iodophenoxy)benzyl)morpholine

2012 ◽  
Vol 11 (4) ◽  
pp. 7290.2011.00049 ◽  
Author(s):  
Naoki Kanegawa ◽  
Yasushi Kiyono ◽  
Taku Sugitaa ◽  
Yuji Kuge ◽  
Yasushisa Fujibayasi ◽  
...  
Keyword(s):  
Rat Model ◽  
Thalamic Nucleus ◽  
Ex Vivo ◽  
Norepinephrine Transporter ◽  
Diagnostic Information ◽  
Brain Diseases ◽  
Imaging Biomarker ◽  
Control Group ◽  
The Brain

To visualize the norepinephrine transporters (NETs) in various brain diseases, we developed radioiodinated (2S,αS)-2-(α-(2-iodophenoxy)benzyl)morpholine ((S,S)-IPBM). This radioligand achieved the basic requirements for NET imaging. In this study, we assessed the potential of radioiodinated (S,S)-IPBM as an imaging biomarker of NET to obtain diagnostic information about depression in relation to NET expression in the brain using a rat depression model. The ex vivo autoradiographic experiments using the (S,S)-[125I]IPBM showed significantly lower accumulation of radioactivity in the locus coeruleus (LC) and the anteroventricular thalamic nucleus (AVTN) of the depression group than in those of the control group. Consequently, in vitro autoradiographic experiments showed that NET maximum binding (Bmax) values in the LC and AVTN, known as NET-rich regions, were significantly decreased in the rat model of depression when compared to those of the control rats. In addition, there was an extremely good correlation between NET Bmax and (S,S)-IPBM accumulation ( r = .98), an indication of radioiodinated IPBM as a quantitative NET imaging biomarker. The reduction in(S,S)-[125I]IPBM accumulation in the rat model of depression correlated with that of NET density. These results suggest that (S,S)-[123I]IPBM has potential as an imaging biomarker of NET to obtain diagnostic information about major depression.

scholarly journals Mechanical properties of porcine brain tissue in vivo and ex vivo estimated by MR elastography

2018 ◽  
Vol 69 ◽  
pp. 10-18 ◽  
Author(s):  
Charlotte A. Guertler ◽  
Ruth J. Okamoto ◽  
John L. Schmidt ◽  
Andrew A. Badachhape ◽  
Curtis L. Johnson ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Brain Tissue ◽  
Ex Vivo ◽  
Mr Elastography ◽  
Porcine Brain ◽  
Porcine Brain Tissue

Study of brain and whole blood PK/PD of bortezomib in rat models

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 12036-12036 ◽  
Author(s):  
L. J. Yu ◽  
B. Riordan ◽  
P. Hatsis ◽  
A. Brockman ◽  
S. Daniels ◽  
...  
Keyword(s):  
Whole Blood ◽  
Ex Vivo ◽  
Proteasome Inhibition ◽  
Proteasome Activity ◽  
Constant Infusion ◽  
Vehicle Group ◽  
20S Proteasome ◽  
Blood Contamination ◽  
The Brain ◽  
Brain Tissues

12036 Background: Bortezomib (Btz) is the first in class proteasome inhibitor and has been approved for treatment of multiple myeloma patients who have received at least 1 prior therapy. The potential for Btz to penetrate the CNS was examined in the rat under two conditions: intact blood brain barrier (BBB) and compromised BBB induced by middle cerebral artery occlusion (MCAO). Methods: Intact BBB: healthy rats received an iv bolus followed by a constant infusion of Btz to steady-state (SS). The blood and brain samples were collected for determination of concentration (Conc) of Btz (PK) and of 20S proteasome activity (PD). The brain samples were collected after a perfusion with saline. Inulin was included in the study in order to determine blood contamination in brain tissues. Compromised BBB: rats underwent MCAO surgery and 1 h later were administered Btz via an iv bolus of 0, 0.1, 0.2 or 0.35 mg/kg. The blood and brain samples were collected through 24 h postdose. Conc of Btz was determined by an LC/MS/MS method. The 20S proteasome activity was measured using an ex vivo assay. Results: 1) Intact BBB: at SS the mean blood Conc of Btz was 140 ng/mL, and proteasome activity in the blood was inhibited by ∼80% compared to the vehicle group (p < 0.0001). In contrast, the brain Conc of Btz was extremely low (∼3 ng/g) with the brain-to-blood ratio of ∼0.02. No difference was observed in brain proteasome activity between the vehicle and Btz-treated groups. 2)The MCAO rat: the PK/PD relationship in the blood was best described by a sigmoid Emax model with an EC50 of 110 ng/mL and gamma factor of 3.8. The model also suggests that there is no proteasome inhibition (PI) when the blood Conc is <40 ng/mL (no effective blood Conc, NOEBC). In the brain, the Cmax of Btz was 22.0 ng/g from the highest dose group, in contrast to the blood Cmax of 164 ng/mL. The increased exposure in the brain of a MCAO rat relative to a normal rat was anticipated as its BBB is impaired. However, the brain Concs were all below the NOEBC. No significant PI was observed in all brain tissues (P>0.1). Conclusion: Very poor brain penetration was observed for Btz in rats. Btz showed PI in whole blood but not in the brain either of normal or MCAO rats following administration of Btz. [Table: see text]

Neuroimaging

2013 ◽  
Author(s):  
Claire E. Sexton ◽  
Verena Heise ◽  
Klaus P. Ebmeier
Keyword(s):  
Old Age ◽  
Clinical Applications ◽  
Structure And Function ◽  
Brain Diseases ◽  
Resonance Imaging ◽  
Short Summary ◽  
Old Age Psychiatry ◽  
And Function ◽  
The Brain ◽  
Potential Use

Neuroimaging provides a way of examining the structure and function of the brain in life. This chapter gives an up-to-date summary of the methods employed in research and clinic, what is involved for the patient in taking part in imaging, and the clinical applications, both current and about to enter general old age psychiatry practice. Magnetic resonance imaging and imaging with ionising radiation, such as CT, SPECT and PET, are covered. In addition we give a short summary of the applications and potential of electro- and magneto-physiological techniques. We summarise the current and potential use of neuroimaging methods in diagnosis, prognosis, understanding illness mechanisms and the brain mechanisms that confer resilience against the brain diseases of old age.

scholarly journals Rapid Shear Stress-dependent ENaC Membrane Insertion is Mediated by the Endothelial Glycocalyx and the Mineralocorticoid Receptor

2021 ◽  
Author(s):  
Zülfü C. Cosgun ◽  
Magdalena Sternak ◽  
Benedikt Fels ◽  
Anna Bar ◽  
Grzegorz Kwiatkowski ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Mineralocorticoid Receptor ◽  
Ex Vivo ◽  
Endothelial Glycocalyx ◽  
Membrane Insertion ◽  
Physiological Conditions

Abstract The contribution of the shear-stress sensitive epithelial Na+ channel (ENaC) to the mechanical properties of the endothelial cell surface under (patho)physiological conditions is unclear. This issue was addressed in in vivo and in vitro models for endothelial dysfunction. Cultured human umbilical vein endothelial cells (HUVEC) were exposed to laminar (LSS) or non-laminar shear stress (NLSS). ENaC membrane insertion was quantified using Quantum-dot-based immunofluorescence staining and the mechanical properties of the cell surface were probed with the Atomic Force Microscope (AFM) in vitro and ex vivo in isolated aortae of C57BL/6 and ApoE/LDLR-/- mice. Flow- and acetylcholine-mediated vasodilation were measured in vivo using magnetic resonance imaging. Acute LSS led to a rapid mineralocorticoid receptor (MR)-dependent membrane insertion of ENaC and subsequent stiffening of the endothelial cortex caused by actin polymerization. Of note, NLSS stress further augmented the cortical stiffness of the cells. These effects strongly depend on the presence of the endothelial glycocalyx (eGC) and could be prevented by functional inhibition of ENaC and MR in vitro and ex vivo endothelial cells derived from C57BL/6 and ApoE/LDLR-/- vessel. As expected, in vivo in C57BL/6 vessels ENaC- and MR-inhibtion blunted flow- and acetylcholine-mediated vasodilation, while in the dysfunctional ApoE/LDLR-/- vessels this effect was absent. In conclusion, under physiological conditions, endothelial ENaC, together with the glycocalyx, was identified as an important shear stress sensor and mediator of endothelium-dependent vasodilation. In contrast, in pathophysiological conditions, ENaC-mediated mechanotransduction and endothelium-dependent vasodilation were lost, contributing to sustained endothelial stiffening and dysfunction.

Characterization of Ischemic Stroke in CT Images using Image Processing

2017 ◽  
Vol 7 (9) ◽  
pp. 18
Author(s):  
Amal Alzain ◽  
Suhaib Alameen ◽  
Rani Elmaki ◽  
Mohamed E. M. Gar-Elnabi
Keyword(s):  
Ischemic Stroke ◽  
White Matter ◽  
Classification Accuracy ◽  
Ct Images ◽  
Gray Level ◽  
Level Variation ◽  
Interactive Data ◽  
The Brain ◽  
Brain Tissues

This study concern to characterize the brain tissues to ischemic stroke, gray matter, white matter and CSF using texture analysisto extract classification features from CT images. The First Order Statistic techniques included sevenfeatures. To find the gray level variation in CT images it complements the FOS features extracted from CT images withgray level in pixels and estimate the variation of thesubpatterns. analyzing the image with Interactive Data Language IDL software to measure the grey level of images. The results show that the Gray Level variation and   features give classification accuracy of ischemic stroke 97.6%, gray matter95.2%, white matter 97.3% and the CSF classification accuracy 98.0%. The overall classification accuracy of brain tissues 97.0%.These relationships are stored in a Texture Dictionary that can be later used to automatically annotate new CT images with the appropriate brain tissues names.

Hydrogels as Antibacterial Biomaterials

2018 ◽  
Vol 24 (8) ◽  
pp. 843-854 ◽  
Author(s):  
Weiguo Xu ◽  
Shujun Dong ◽  
Yuping Han ◽  
Shuqiang Li ◽  
Yang Liu
Keyword(s):  
Mechanical Properties ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Water Content ◽  
Oxygen Permeability ◽  
Structural Diversity ◽  
High Water ◽  
Clinical Applications ◽  
High Water Content ◽  
Biomedical Field

Hydrogels, as a class of materials for tissue engineering and drug delivery, have high water content and solid-like mechanical properties. Currently, hydrogels with an antibacterial function are a research hotspot in biomedical field. Many advanced antibacterial hydrogels have been developed, each possessing unique qualities, namely high water swellability, high oxygen permeability, improved biocompatibility, ease of loading and releasing drugs and structural diversity. In this article, an overview is provided on the preparation and applications of various antibacterial hydrogels. Furthermore, the prospects in biomedical researches and clinical applications are predicted.

A new method to predict anomaly in brain network based on graph deep learning

2020 ◽  
Vol 31 (6) ◽  
pp. 681-689
Author(s):  
Jalal Mirakhorli ◽  
Hamidreza Amindavar ◽  
Mojgan Mirakhorli
Keyword(s):  
Deep Learning ◽  
Large Scale ◽  
Brain Plasticity ◽  
Brain Network ◽  
High Order ◽  
Brain Diseases ◽  
Simultaneous Occurrence ◽  
Generative Adversarial Network ◽  
The Brain ◽  
Brain Connections

AbstractFunctional magnetic resonance imaging a neuroimaging technique which is used in brain disorders and dysfunction studies, has been improved in recent years by mapping the topology of the brain connections, named connectopic mapping. Based on the fact that healthy and unhealthy brain regions and functions differ slightly, studying the complex topology of the functional and structural networks in the human brain is too complicated considering the growth of evaluation measures. One of the applications of irregular graph deep learning is to analyze the human cognitive functions related to the gene expression and related distributed spatial patterns. Since a variety of brain solutions can be dynamically held in the neuronal networks of the brain with different activity patterns and functional connectivity, both node-centric and graph-centric tasks are involved in this application. In this study, we used an individual generative model and high order graph analysis for the region of interest recognition areas of the brain with abnormal connection during performing certain tasks and resting-state or decompose irregular observations. Accordingly, a high order framework of Variational Graph Autoencoder with a Gaussian distributer was proposed in the paper to analyze the functional data in brain imaging studies in which Generative Adversarial Network is employed for optimizing the latent space in the process of learning strong non-rigid graphs among large scale data. Furthermore, the possible modes of correlations were distinguished in abnormal brain connections. Our goal was to find the degree of correlation between the affected regions and their simultaneous occurrence over time. We can take advantage of this to diagnose brain diseases or show the ability of the nervous system to modify brain topology at all angles and brain plasticity according to input stimuli. In this study, we particularly focused on Alzheimer’s disease.

scholarly journals Millimeter (MM) wave and microwave frequency radiation produce deeply penetrating effects: the biology and the physics

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Martin L. Pall
Keyword(s):  
Magnetic Fields ◽  
Electric Fields ◽  
Cardiac Activity ◽  
Maximum Level ◽  
Voltage Sensor ◽  
The Body ◽  
Time Varying ◽  
The Brain ◽  
Mm Waves

Abstract Millimeter wave (MM-wave) electromagnetic fields (EMFs) are predicted to not produce penetrating effects in the body. The electric but not magnetic part of MM-EMFs are almost completely absorbed within the outer 1 mm of the body. Rodents are reported to have penetrating MM-wave impacts on the brain, the myocardium, liver, kidney and bone marrow. MM-waves produce electromagnetic sensitivity-like changes in rodent, frog and skate tissues. In humans, MM-waves have penetrating effects including impacts on the brain, producing EEG changes and other neurological/neuropsychiatric changes, increases in apparent electromagnetic hypersensitivity and produce changes on ulcers and cardiac activity. This review focuses on several issues required to understand penetrating effects of MM-waves and microwaves: 1. Electronically generated EMFs are coherent, producing much higher electrical and magnetic forces then do natural incoherent EMFs. 2. The fixed relationship between electrical and magnetic fields found in EMFs in a vacuum or highly permeable medium such as air, predicted by Maxwell’s equations, breaks down in other materials. Specifically, MM-wave electrical fields are almost completely absorbed in the outer 1 mm of the body due to the high dielectric constant of biological aqueous phases. However, the magnetic fields are very highly penetrating. 3. Time-varying magnetic fields have central roles in producing highly penetrating effects. The primary mechanism of EMF action is voltage-gated calcium channel (VGCC) activation with the EMFs acting via their forces on the voltage sensor, rather than by depolarization of the plasma membrane. Two distinct mechanisms, an indirect and a direct mechanism, are consistent with and predicted by the physics, to explain penetrating MM-wave VGCC activation via the voltage sensor. Time-varying coherent magnetic fields, as predicted by the Maxwell–Faraday version of Faraday’s law of induction, can put forces on ions dissolved in aqueous phases deep within the body, regenerating coherent electric fields which activate the VGCC voltage sensor. In addition, time-varying magnetic fields can directly put forces on the 20 charges in the VGCC voltage sensor. There are three very important findings here which are rarely recognized in the EMF scientific literature: coherence of electronically generated EMFs; the key role of time-varying magnetic fields in generating highly penetrating effects; the key role of both modulating and pure EMF pulses in greatly increasing very short term high level time-variation of magnetic and electric fields. It is probable that genuine safety guidelines must keep nanosecond timescale-variation of coherent electric and magnetic fields below some maximum level in order to produce genuine safety. These findings have important implications with regard to 5G radiation.

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