Phosphobisaromatic motifs enable rapid enzymatic self-assembly and hydrogelation of short peptides

Soft Matter ◽  
2021 ◽  
Author(s):  
Meihui Yi ◽  
Jiaqi Guo ◽  
Hongjian He ◽  
Weiyi Tan ◽  
Nya Harmon ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Self Assembly ◽  
Soft Materials ◽  
Short Peptides

Enzyme-instructed self-assembly (EISA) and hydrogelation is a versatile approach for generating soft materials. Most of the substrates for alkaline phosphatase catalysed EISA utilizes phosphotyrosine (pTyr) as the enzymatic trigger for...

Interrelationship of the cellular distribution and secretion of regular structure (RS) protein in Bacillus licheniformis nm 105

1992 ◽  
Vol 50 (1) ◽  
pp. 712-713
Author(s):  
Xiaorong Zhu ◽  
Richard McVeigh ◽  
Bijan K. Ghosh
Keyword(s):  
Alkaline Phosphatase ◽  
Bacillus Licheniformis ◽  
Self Assembly ◽  
Gel Electrophoresis ◽  
Culture Supernatant ◽  
Regular Structure ◽  
The Self ◽  
Cellular Distribution ◽  
Structural Protein ◽  
Laboratory Cultures

A mutant of Bacillus licheniformis 749/C, NM 105 exhibits some notable properties, e.g., arrest of alkaline phosphatase secretion and overexpression and hypersecretion of RS protein. Although RS is known to be widely distributed in many microbes, it is rarely found, with a few exceptions, in laboratory cultures of microorganisms. RS protein is a structural protein and has the unusual properties to form aggregate. This characteristic may have been responsible for the self assembly of RS into regular tetragonal structures. Another uncommon characteristic of RS is that enhanced synthesis and secretion which occurs when the cells cease to grow. Assembled RS protein with a tetragonal structure is not seen inside cells at any stage of cell growth including cells in the stationary phase of growth. Gel electrophoresis of the culture supernatant shows a very large amount of RS protein in the stationary culture of the B. licheniformis. It seems, Therefore, that the RS protein is cotranslationally secreted and self assembled on the envelope surface.

Aromatic identity, electronic substitution, and sequence in amphiphilic tripeptide self-assembly

Soft Matter ◽  
2018 ◽  
Vol 14 (45) ◽  
pp. 9168-9174 ◽  
Author(s):  
Jugal Kishore Sahoo ◽  
Calvin Nazareth ◽  
Michael A. VandenBerg ◽  
Matthew J. Webber
Keyword(s):  
Self Assembly ◽  
Short Peptides ◽  
Design Rules ◽  
Sequence Variations

The design rules for self-assembly of short peptides are assessed using a combination of chemical and sequence variations.

scholarly journals SAPdb: A database of short peptides and the corresponding nanostructures formed by self-assembly

2021 ◽  
pp. 104391
Author(s):  
Deepika Mathur ◽  
Harpreet Kaur ◽  
Anjali Dhall ◽  
Neelam Sharma ◽  
Gajendra P.S. Raghava
Keyword(s):  
Self Assembly ◽  
Short Peptides

scholarly journals Chirality, Gelation Ability and Crystal Structure: Together or Apart? Alkyl Phenyl Ethers of Glycerol as Simple LMWGs

Symmetry ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 732
Author(s):  
Alexander A. Bredikhin ◽  
Aidar T. Gubaidullin ◽  
Zemfira A. Bredikhina ◽  
Robert R. Fayzullin ◽  
Olga A. Lodochnikova
Keyword(s):  
Self Assembly ◽  
Chiral Recognition ◽  
Soft Materials ◽  
Gelling Agent ◽  
Low Molecular Weight ◽  
X Ray Diffraction ◽  
X Ray ◽  
Different Types ◽  
Low Molecular Weight Gelators ◽  
Phenyl Ethers

Chiral recognition plays an important role in the self-assembly of soft materials, in particular supramolecular organogels formed by low molecular weight gelators (LMWGs). Out of 14 pairs of the studied racemic and enantiopure samples of alkyl-substituted phenyl ethers of glycerol, only eight enantiopure diols form the stable gels in nonane. The formation of gels from solutions was studied by polarimetry, and their degradation with the formation of xerogels was studied by the PXRD method. The revealed crystalline characteristics of all studied xerogels corresponded to those for crystalline samples of the parent gelators. In addition to those previously investigated, crystalline samples of enantiopure para-n-alkylphenyl glycerol ethers [alkyl = pentyl (5), hexyl (6), heptyl (7), octyl (8), nonyl (9)] and racemic 3-(3,5-dimethylphenoxy)propane-1,2-diol (rac-14) have been examined by single crystal X-ray diffraction analysis. Among 22 samples of compounds 1-14 studied by SC-XRD, seven different types of supramolecular motifs are identified, of which only two are realized in crystals of supramolecular gelators. An attempt was made to relate the ability to gel formation with the characteristics of the supramolecular motif of a potential gelling agent, and the frequency of formation of the motif, required for gelation, with the chiral characteristics of the sample.

Lysosome‐Targeted and Fluorescence‐Turned “On” Cytotoxicity Induced by Alkaline Phosphatase‐Triggered Self‐Assembly

2021 ◽  
pp. 2101346
Author(s):  
Chengfan Wu ◽  
Chenchen Wang ◽  
Tong Zhang ◽  
Ge Gao ◽  
Mengxing Wei ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Self Assembly

scholarly journals Thermodynamics of structured fluids. Hard science for soft materials

2000 ◽  
Vol 72 (10) ◽  
pp. 1819-1834 ◽  
Author(s):  
John M. Prausnitz
Keyword(s):  
Self Assembly ◽  
Theoretical Calculations ◽  
Soft Materials ◽  
Confined Space ◽  
Molecular Physics ◽  
Fluid Structure ◽  
Light Emitting ◽  
Structured Fluids ◽  
Recent Developments ◽  
Narrow Space

At liquid-like densities, molecules of complex fluids can assume a variety of structures (or positions) in space; when the molecules contain many atoms as, for example, in polymers, that variety becomes very large. Further, when confined to a narrow space, it is possible to achieve structures that are not normally observed. Thanks to recent advances in statistical mechanics and molecular physics, and thanks to increasingly fast computers, it is now possible to calculate a fluid's structure, that is, the positions of molecules at equilibrium under given conditions. Calculation of fluid structure is useful because thermodynamic properties depend strongly on that structure, leading to possible applications for new materials. Three examples illustrate some recent developments; each example is presented only schematically (with a minimum of equations) to indicate the physical basis of the mathematical description. The first example considers the effect of branching on self-assembly (micellization) of copolymers (with possible long-range applications in medicine). The second and third examples consider the effect of confinement on fluid structure: first, crystallization in a narrow, confined space to produce a desired crystal structure (with possible applications for light-emitting diodes) and second, suppression of micellization of a diblock copolymer in a thin film (with possible application in lithography). Whenever possible, theoretical calculations are compared with experimental results.

Tuning the Self-Assembly of Short Peptides via Sequence Variations

Langmuir ◽  
2013 ◽  
Vol 29 (44) ◽  
pp. 13457-13464 ◽  
Author(s):  
Yurong Zhao ◽  
Jiqian Wang ◽  
Li Deng ◽  
Peng Zhou ◽  
Shengjie Wang ◽  
...  
Keyword(s):  
Self Assembly ◽  
The Self ◽  
Short Peptides ◽  
Sequence Variations

scholarly journals Organic/Inorganic hydrogels by simultaneous self-assembly and mineralization of aromatic short-peptides

2022 ◽  
Author(s):  
Mari C. Mañas-Torres ◽  
Gloria Belén Ramírez-Rodríguez ◽  
Jose I. Garcia-Peiro ◽  
Belen Parra-Torrejón ◽  
Juan Manuel Cuerva ◽  
...  
Keyword(s):  
Self Assembly ◽  
Short Peptides ◽  
Self Assembled

Self-assembled peptides and proteins have turned out to be excellent templates for the growth of inorganic minerals trying to emulate natural biomineralization processes. Doing this, researchers have developed complex sophisticate...

Enzyme-instructed self-assembly enabled fluorescence light-up for alkaline phosphatase detection

Talanta ◽  
2021 ◽  
pp. 123078
Author(s):  
Yiming Zhang ◽  
Yinghao Ding ◽  
Xinxin Li ◽  
Zhenghao Zhang ◽  
Xiangyang Zhang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Self Assembly ◽  
Fluorescence Light
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