scholarly journals Tuning the charge of polyelectrolyte complex membranes prepared via aqueous phase separation

Soft Matter ◽  
2021 ◽  
Author(s):  
Elif Nur Durmaz ◽  
Joshua D. Willott ◽  
Md Mizanul Haque Mizan ◽  
Wiebe M. de Vos
Keyword(s):  
Phase Separation ◽  
Aqueous Phase ◽  
Polyelectrolyte Complex ◽  
Tuning Parameter ◽  
Mixing Ratio ◽  
Selective Retention ◽  
Charged Membranes

Positively and negatively charged membranes are obtained by simply changing the mixing ratio of the polyelectrolytes in a fully aqueous preparation approach. This leads to selective retention of asymmetric salts and a new membrane tuning parameter.

scholarly journals Polyelectrolyte Complex Membranes via Salinity Change Induced Aqueous Phase Separation

2020 ◽  
Vol 2 (7) ◽  
pp. 2612-2621 ◽  
Author(s):  
Elif Nur Durmaz ◽  
Muhammad Irshad Baig ◽  
Joshua D. Willott ◽  
Wiebe M. de Vos
Keyword(s):  
Phase Separation ◽  
Aqueous Phase ◽  
Polyelectrolyte Complex ◽  
Salinity Change

scholarly journals Polyelectrolyte Complex Hollow Fiber Membranes Prepared via Aqueous Phase Separation

2022 ◽  
Author(s):  
Muhammad Irshad Baig ◽  
Mehdi Pejman ◽  
Joshua D. Willott ◽  
Alberto Tiraferri ◽  
Wiebe M. de Vos
Keyword(s):  
Phase Separation ◽  
Aqueous Phase ◽  
Hollow Fiber ◽  
Polyelectrolyte Complex ◽  
Hollow Fiber Membranes ◽  
Fiber Membranes

scholarly journals Glycosyl-phosphatidylinositol-anchored membrane proteins can be distinguished from transmembrane polypeptide-anchored proteins by differential solubilization and temperature-induced phase separation in Triton X-114

1991 ◽  
Vol 280 (3) ◽  
pp. 745-751 ◽  
Author(s):  
N M Hooper ◽  
A Bashir
Keyword(s):  
Phase Separation ◽  
Membrane Proteins ◽  
Aqueous Phase ◽  
Hydrophobic Membrane ◽  
Rich Phase ◽  
Glycosyl Phosphatidylinositol ◽  
Ionic Detergent ◽  
Membrane Spanning ◽  
Triton X ◽  
Insoluble Pellet

Treatment of kidney microvillar membranes with the non-ionic detergent Triton X-114 at 0 degrees C, followed by low-speed centrifugation, generated a detergent-insoluble pellet and a detergent-soluble supernatant. The supernatant was further fractionated by phase separation at 30 degrees C into a detergent-rich phase and a detergent-depleted or aqueous phase. Those ectoenzymes with a covalently attached glycosyl-phosphatidylinositol (G-PI) membrane anchor were recovered predominantly (greater than 73%) in the detergent-insoluble pellet. In contrast, those ectoenzymes anchored by a single membrane-spanning polypeptide were recovered predominantly (greater than 62%) in the detergent-rich phase. Removal of the hydrophobic membrane-anchoring domain from either class of ectoenzyme resulted in the proteins being recovered predominantly (greater than 70%) in the aqueous phase. This technique was also applied to other membrane types, including pig and human erythrocyte ghosts, where, in both cases, the G-PI-anchored acetylcholinesterase partitioned predominantly (greater than 69%) into the detergent-insoluble pellet. When the microvillar membranes were subjected only to differential solubilization with Triton X-114 at 0 degrees C, the G-PI-anchored ectoenzymes were recovered predominantly (greater than 63%) in the detergent-insoluble pellet, whereas the transmembrane-polypeptide-anchored ectoenzymes were recovered predominantly (greater than 95%) in the detergent-solubilized supernatant. Thus differential solubilization and temperature-induced phase separation in Triton X-114 distinguished between G-PI-anchored membrane proteins, transmembrane-polypeptide-anchored proteins and soluble, hydrophilic proteins. This technique may be more useful and reliable than susceptibility to release by phospholipases as a means of identifying a G-PI anchor on an unpurified membrane protein.

scholarly journals The hyaluronate synthase from a eukaryotic cell line

1993 ◽  
Vol 290 (3) ◽  
pp. 791-795 ◽  
Author(s):  
L Klewes ◽  
E A Turley ◽  
P Prehm
Keyword(s):  
Monoclonal Antibodies ◽  
Phase Separation ◽  
Affinity Chromatography ◽  
Cell Line ◽  
Aqueous Phase ◽  
Plasma Membranes ◽  
Eukaryotic Cell ◽  
Molecular Masses ◽  
Triton X ◽  
Hyaluronate Synthase

The hyaluronate synthase complex was identified in plasma membranes from B6 cells. It contained two subunits of molecular masses 52 kDa and 60 kDa which bound the precursor UDP-GlcA in digitonin solution and partitioned into the aqueous phase, together with nascent hyaluronate upon Triton X-114 phase separation. The 52 kDa protein cross-reacted with poly- and monoclonal antibodies raised against the streptococcal hyaluronate synthase and the 60 kDa protein was recognized by monoclonal antibodies raised against a hyaluronate receptor. The 52 kDa protein was purified to homogeneity by affinity chromatography with monoclonal anti-hyaluronate synthase.

scholarly journals Powerful Concentration of Rhodium in Plating Wastewater Using Homogeneous Liquid–Liquid Extraction (HoLLE) and Study for Scale-up

2017 ◽  
Vol 7 (4) ◽  
pp. 44 ◽  
Author(s):  
Takeshi Kato ◽  
Shotaro Saito ◽  
Shigekatsu Oshite ◽  
Shukuro Igarashi
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Aqueous Phase ◽  
Scale Up ◽  
Volume Ratio ◽  
Liquid Extraction ◽  
Optimum Conditions ◽  
Homogeneous Liquid ◽  
Liquid Liquid Extraction ◽  
Plating Wastewater

A powerful technique for the concentration of rhodium (Rh) in plating wastewater was developed. The technique entails complexing Rh with 1-(2-pyridylazo)-2-naphthol (PAN) followed by homogeneous liquid–liquid extraction (HoLLE) with Zonyl FSA. The optimum HoLLE conditions were determined as follows: [ethanol]T = 30.0 vol.%, pH = 4.00, and Rh:PAN = 1:5. Under these optimum conditions, 88.1% of Rh was extracted into the sedimented liquid phase. After phase separation, the volume ratio [aqueous phase (Va) /sedimented liquid phase (Vs)] of Va and Vs was 1000 (50 mL → 0.050 mL). We then applied the new method to wastewater generated by the plating industry. The phase separation was satisfactorily achieved when the volume was scaled up to 1000 mL of the actual wastewater; 84.7% of Rh was extracted into the sedimented liquid phase. After phase separation, Va/Vs was 588 (1000 mL - 1.70 mL).

Rapid Multilevel Compartmentalization of Stable All-Aqueous Blastosomes by Interfacial Aqueous-Phase Separation

ACS Nano ◽  
2020 ◽  
Vol 14 (9) ◽  
pp. 11215-11224
Author(s):  
Shipei Zhu ◽  
Joe Forth ◽  
Ganhua Xie ◽  
Youchuang Chao ◽  
Jingxuan Tian ◽  
...  
Keyword(s):  
Phase Separation ◽  
Aqueous Phase
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