scholarly journals Investigating Lipid Headgroup Composition within Epithelial Membranes: A Systematic Review

Soft Matter ◽  
2021 ◽  
Author(s):  
Ryan Thomas Coones ◽  
Rebecca Green ◽  
Richard Andrew Frazier
Keyword(s):  
Systematic Review ◽  
Lipid Composition ◽  
Biological Membranes ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Epithelial Membranes ◽  
Insight Into

Membrane lipid composition is often quoted within the literature, but with very little insight into how or why these compositions vary when compared to other biological membranes. One prominent area...

Influence of membrane lipid composition on the structure and activity of γ-secretase

2018 ◽  
Vol 20 (43) ◽  
pp. 27294-27304 ◽  
Author(s):  
Rodrigo Aguayo-Ortiz ◽  
John E. Straub ◽  
Laura Dominguez
Keyword(s):  
Computational Modeling ◽  
Lipid Composition ◽  
Membrane Lipid ◽  
Membrane Lipid Composition ◽  
Bilayer Lipid ◽  
Modeling Approaches ◽  
And Function ◽  
Structural Ensemble ◽  
Structure And Activity ◽  
Insight Into

Insight into the effect of bilayer lipid composition on the GS structural ensemble and function using multiscale computational modeling approaches.

scholarly journals Membrane Lipid Composition of the Moderately Thermophilic Ammonia-Oxidizing Archaeon “Candidatus Nitrosotenuis uzonensis” at Different Growth Temperatures

2019 ◽  
Vol 85 (20) ◽  
Author(s):  
Nicole J. Bale ◽  
Marton Palatinszky ◽  
W. Irene C. Rijpstra ◽  
Craig W. Herbold ◽  
Michael Wagner ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Strong Influence ◽  
Thermal Spring ◽  
Membrane Lipid ◽  
Effect Of Temperature ◽  
Environmental Niche ◽  
Membrane Lipid Composition ◽  
Moderately Thermophilic ◽  
Wide Range ◽  
Membrane Spanning

ABSTRACT “Candidatus Nitrosotenuis uzonensis” is the only cultured moderately thermophilic member of the thaumarchaeotal order Nitrosopumilales (NP) that contains many mesophilic marine strains. We examined its membrane lipid composition at different growth temperatures (37°C, 46°C, and 50°C). Its lipids were all membrane-spanning glycerol dialkyl glycerol tetraethers (GDGTs), with 0 to 4 cyclopentane moieties. Crenarchaeol (cren), the characteristic thaumarchaeotal GDGT, and its isomer (crenʹ) were present in high abundance (30 to 70%). The GDGT polar headgroups were mono-, di-, and trihexoses and hexose/phosphohexose. The ratio of glycolipid to phospholipid GDGTs was highest in the cultures grown at 50°C. With increasing growth temperatures, the relative contributions of cren and crenʹ increased, while those of GDGT-0 to GDGT-4 (including isomers) decreased. TEX86 (tetraether index of tetraethers consisting of 86 carbons)-derived temperatures were much lower than the actual growth temperatures, further demonstrating that TEX86 does not accurately reflect the membrane lipid adaptation of thermophilic Thaumarchaeota. As the temperature increased, specific GDGTs changed relative to their isomers, possibly representing temperature adaption-induced changes in cyclopentane ring stereochemistry. Comparison of a wide range of thaumarchaeotal core lipid compositions revealed that the “Ca. Nitrosotenuis uzonensis” cultures clustered separately from other members of the NP order and the Nitrososphaerales (NS) order. While phylogeny generally seems to have a strong influence on GDGT distribution, our analysis of “Ca. Nitrosotenuis uzonensis” demonstrates that its terrestrial, higher-temperature niche has led to a lipid composition that clearly differentiates it from other NP members and that this difference is mostly driven by its high crenʹ content. IMPORTANCE For Thaumarchaeota, the ratio of their glycerol dialkyl glycerol tetraether (GDGT) lipids depends on growth temperature, a premise that forms the basis of the widely applied TEX86 paleotemperature proxy. A thorough understanding of which GDGTs are produced by which Thaumarchaeota and what the effect of temperature is on their GDGT composition is essential for constraining the TEX86 proxy. “Ca. Nitrosotenuis uzonensis” is a moderately thermophilic thaumarchaeote enriched from a thermal spring, setting it apart in its environmental niche from the other marine mesophilic members of its order. Indeed, we found that the GDGT composition of “Ca. Nitrosotenuis uzonensis” cultures was distinct from those of other members of its order and was more similar to those of other thermophilic, terrestrial Thaumarchaeota. This suggests that while phylogeny has a strong influence on GDGT distribution, the environmental niche that a thaumarchaeote inhabits also shapes its GDGT composition.

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