Depletion forces drive reversible capture of live bacteria on non-adhesive surfaces

Soft Matter ◽  
2021 ◽  
Author(s):  
Wuqi Amy Niu ◽  
Sylvia L. Rivera ◽  
M. Sloan Siegrist ◽  
Maria M. Santore
Keyword(s):  
Underlying Surface ◽  
Reversible Adhesion ◽  
Adhesive Surface ◽  
Surface Character ◽  
Live Bacteria ◽  
Living Bacteria ◽  
Depletion Forces

Dissolved polymer can drive the reversible adhesion of living bacteria on a non-adhesive surface, defeating the non-adhesive underlying surface character.

THE USE OF THE DRUG «LIVE BACTERIA» FOR BEES FEEDING IN WINTER

2019 ◽  
Vol 1 (3) ◽  
pp. 335-341
Author(s):  
G.M. Kryukovskaya ◽  
◽  
N.Y. Sysoeva ◽  
I.G. Glamazdin ◽  
T.O. Maryushina ◽  
...  
Keyword(s):  
Survival Rate ◽  
Honey Bees ◽  
Intestinal Tract ◽  
Intestinal Wall ◽  
Biological Product ◽  
Opportunistic Bacteria ◽  
Intestinal Contents ◽  
Live Bacteria ◽  
Living Bacteria

The purpose of the study is to evaluate the effectiveness of the probiotic biological product "Living bacteria" for feeding bees in winter. The researches were carried out on honey bees of the Central Russian breed on private apiaries in Moscow and Pskov regions. The bees of experienced families who received feeding with probiotics the intestinal wall was elastic, the intestinal tract was removed easily and entirely, the excrements were dense and homogeneous. The intestines of bees from control families were filled with liquid excrement, its walls were loose. After using of probiotics, the processes of digestion and evacuation of intestinal contents improved, the content of undigested pollen, detritus and opportunistic bacteria decreased in the excrements. The survival rate of bees in winter has increased.

Evaluating H2O2 in Living Bacteria by Ratiometric Fluorescent Probe

2022 ◽  
Vol 905 ◽  
pp. 210-217
Author(s):  
Qian Qian Chen
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Gram Negative Bacteria ◽  
Signal Molecule ◽  
Gram Negative ◽  
Key Factor ◽  
Live Bacteria ◽  
First Time ◽  
Living Bacteria ◽  
Ratiometric Fluorescent Probe

Hydrogen peroxide (H2O2) is a significant signal molecule in physiological and pathological processes. Levels of H2O2 in bacteria are proved to be a key factor in immune response. To sum up, detection of H2O2 levels in living bacteria is remarkable for further study of its physiological and pathological effects. Herein, we propose a novel ratiometric fluorescent probe (Nahp) to detect H2O2 in living cells and bacteria. In addition, based on boronate, Nahp has satisfactory selectivity and sensitivity toward H2O2 (LOD = 0.158 μM). Furthermore, with excellent detection performance to H2O2, Nahp is successfully used for fluorescent bioimaging of H2O2 and measuring H2O2 accumulation in bacteria. Most importantly, the probe was also used to image H2O2 in three Gram-negative bacteria, clearly revealing for the first time significant differences in H2O2 expression levels in live bacteria.

Detection of Signals in Nongaussian Nonstationary Interference From the Underlying Surface

2016 ◽  
Vol 5 (4) ◽  
pp. 23-39
Author(s):  
V.F. Kravchenko ◽  
◽  
O.V. Kravchenko ◽  
V.I. Lutsenko ◽  
I.V. Lutsenko ◽  
...  
Keyword(s):  
Underlying Surface ◽  
Detection Of Signals

Bacterial Peptidoglycan Stapling with Functionalized D-Amino Acids

2019 ◽  
Author(s):  
Sylvia L. Rivera ◽  
Akbar Espaillat ◽  
Arjun K. Aditham ◽  
Peyton Shieh ◽  
Chris Muriel-Mundo ◽  
...  
Keyword(s):  
Cell Wall ◽  
Clinical Success ◽  
Bacterial Species ◽  
Enzymatic Reaction ◽  
Amino Acid Derivative ◽  
Wall Structure ◽  
Live Bacteria ◽  
Cross Links ◽  
Osmotic Lysis ◽  
Bacterial Peptidoglycan

Transpeptidation reinforces the structure of cell wall peptidoglycan, an extracellular heteropolymer that protects bacteria from osmotic lysis. The clinical success of transpeptidase-inhibiting β-lactam antibiotics illustrates the essentiality of these cross-linkages for cell wall integrity, but the presence of multiple, seemingly redundant transpeptidases in many bacterial species makes it challenging to determine cross-link function precisely. Here we present a technique to covalently link peptide strands by chemical rather than enzymatic reaction. We employ bio-compatible click chemistry to induce triazole formation between azido- and alkynyl-D-alanine residues that are metabolically installed in the cell walls of Gram-positive and Gram-negative bacteria. Synthetic triazole cross-links can be visualized by substituting azido-D-alanine with azidocoumarin-D-alanine, an amino acid derivative that undergoes fluorescent enhancement upon reaction with terminal alkynes. Cell wall stapling protects the model bacterium Escherichia coli from β-lactam treatment. Chemical control of cell wall structure in live bacteria can provide functional insights that are orthogonal to those obtained by genetics.<br>

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